Aberrant Promoter Hypermethylation of RASSF Family Members in Merkel Cell Carcinoma
Merkel cell carcinoma (MCC) is one of the most aggressive cancers of the skin. RASSFs are a family of tumor suppressors that are frequently inactivated by promoter hypermethylation in various cancers. We studied CpG island promoter hypermethylation in MCC of RASSF2, RASSF5A, RASSF5C and RASSF10 by c...
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doaj-739da518ed1e423bae8de77884a9ba3b2020-11-24T23:29:00ZengMDPI AGCancers2072-66942013-11-01541566157610.3390/cancers5041566cancers5041566Aberrant Promoter Hypermethylation of RASSF Family Members in Merkel Cell CarcinomaAntje M. Richter0Tanja Haag1Sara Walesch2Peter Herrmann-Trost3Wolfgang C. Marsch4Heinz Kutzner5Peter Helmbold6Reinhard H. Dammann7Institute for Genetics, University of Giessen, Giessen D-35392, GermanyInstitute for Genetics, University of Giessen, Giessen D-35392, GermanyInstitute for Genetics, University of Giessen, Giessen D-35392, GermanyInstitute of Pathology, Halle D-06097, GermanyDepartment of Dermatology, University of Halle, Halle D-06120, GermanyDermPath, Friedrichshafen D-88048, GermanyDepartment of Dermatology, University of Heidelberg, Heidelberg D-69120, GermanyInstitute for Genetics, University of Giessen, Giessen D-35392, GermanyMerkel cell carcinoma (MCC) is one of the most aggressive cancers of the skin. RASSFs are a family of tumor suppressors that are frequently inactivated by promoter hypermethylation in various cancers. We studied CpG island promoter hypermethylation in MCC of RASSF2, RASSF5A, RASSF5C and RASSF10 by combined bisulfite restriction analysis (COBRA) in MCC samples and control tissue. We found RASSF2 to be methylated in three out of 43 (7%), RASSF5A in 17 out of 39 (44%, but also 43% in normal tissue), RASSF5C in two out of 26 (8%) and RASSF10 in 19 out of 84 (23%) of the cancer samples. No correlation between the methylation status of the analyzed RASSFs or between RASSF methylation and MCC characteristics (primary versus metastatic, Merkel cell polyoma virus infection, age, sex) was found. Our results show that RASSF2, RASSF5C and RASSF10 are aberrantly hypermethylated in MCC to a varying degree and this might contribute to Merkel cell carcinogenesis.http://www.mdpi.com/2072-6694/5/4/1566merkel celltumor suppressorDNA methylationepigeneticsRASSF |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Antje M. Richter Tanja Haag Sara Walesch Peter Herrmann-Trost Wolfgang C. Marsch Heinz Kutzner Peter Helmbold Reinhard H. Dammann |
spellingShingle |
Antje M. Richter Tanja Haag Sara Walesch Peter Herrmann-Trost Wolfgang C. Marsch Heinz Kutzner Peter Helmbold Reinhard H. Dammann Aberrant Promoter Hypermethylation of RASSF Family Members in Merkel Cell Carcinoma Cancers merkel cell tumor suppressor DNA methylation epigenetics RASSF |
author_facet |
Antje M. Richter Tanja Haag Sara Walesch Peter Herrmann-Trost Wolfgang C. Marsch Heinz Kutzner Peter Helmbold Reinhard H. Dammann |
author_sort |
Antje M. Richter |
title |
Aberrant Promoter Hypermethylation of RASSF Family Members in Merkel Cell Carcinoma |
title_short |
Aberrant Promoter Hypermethylation of RASSF Family Members in Merkel Cell Carcinoma |
title_full |
Aberrant Promoter Hypermethylation of RASSF Family Members in Merkel Cell Carcinoma |
title_fullStr |
Aberrant Promoter Hypermethylation of RASSF Family Members in Merkel Cell Carcinoma |
title_full_unstemmed |
Aberrant Promoter Hypermethylation of RASSF Family Members in Merkel Cell Carcinoma |
title_sort |
aberrant promoter hypermethylation of rassf family members in merkel cell carcinoma |
publisher |
MDPI AG |
series |
Cancers |
issn |
2072-6694 |
publishDate |
2013-11-01 |
description |
Merkel cell carcinoma (MCC) is one of the most aggressive cancers of the skin. RASSFs are a family of tumor suppressors that are frequently inactivated by promoter hypermethylation in various cancers. We studied CpG island promoter hypermethylation in MCC of RASSF2, RASSF5A, RASSF5C and RASSF10 by combined bisulfite restriction analysis (COBRA) in MCC samples and control tissue. We found RASSF2 to be methylated in three out of 43 (7%), RASSF5A in 17 out of 39 (44%, but also 43% in normal tissue), RASSF5C in two out of 26 (8%) and RASSF10 in 19 out of 84 (23%) of the cancer samples. No correlation between the methylation status of the analyzed RASSFs or between RASSF methylation and MCC characteristics (primary versus metastatic, Merkel cell polyoma virus infection, age, sex) was found. Our results show that RASSF2, RASSF5C and RASSF10 are aberrantly hypermethylated in MCC to a varying degree and this might contribute to Merkel cell carcinogenesis. |
topic |
merkel cell tumor suppressor DNA methylation epigenetics RASSF |
url |
http://www.mdpi.com/2072-6694/5/4/1566 |
work_keys_str_mv |
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