Impact of Blood Collection Tubes and Sample Handling Time on Serum and Plasma Metabolome and Lipidome
<i>Background</i>: Metabolomics is emerging as a valuable tool in clinical science. However, one major challenge in clinical metabolomics is the limited use of standardized guidelines for sample collection and handling. In this study, we conducted a pilot analysis of serum and plasma to...
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doaj-73a6fdfbb7244fb88e0547434ee367052020-11-25T00:37:30ZengMDPI AGMetabolites2218-19892018-12-01848810.3390/metabo8040088metabo8040088Impact of Blood Collection Tubes and Sample Handling Time on Serum and Plasma Metabolome and LipidomeCharmion Cruickshank-Quinn0Laura K. Zheng1Kevin Quinn2Russell Bowler3Richard Reisdorph4Nichole Reisdorph5Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USADepartment of Medicine, St. Joseph Hospital, Denver, CO 80218, USASkaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USADepartment of Medicine, National Jewish Health, Denver, CO 80206, USASkaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USASkaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA<i>Background</i>: Metabolomics is emerging as a valuable tool in clinical science. However, one major challenge in clinical metabolomics is the limited use of standardized guidelines for sample collection and handling. In this study, we conducted a pilot analysis of serum and plasma to determine the effects of processing time and collection tube on the metabolome. <i>Methods</i>: Blood was collected in 3 tubes: Vacutainer serum separator tube (SST, serum), EDTA (plasma) and P100 (plasma) and stored at 4 degrees for 0, 0.5, 1, 2, 4 and 24 h prior to centrifugation. Compounds were extracted using liquid-liquid extraction to obtain a hydrophilic and a hydrophobic fraction and analyzed using liquid chromatography mass spectrometry. Differences among the blood collection tubes and sample processing time were evaluated (ANOVA, Bonferroni FWER ≤ 0.05 and ANOVA, Benjamini Hochberg FDR ≤ 0.1, respectively). <i>Results</i>: Among the serum and plasma tubes 93.5% of compounds overlapped, 382 compounds were unique to serum and one compound was unique to plasma. There were 46, 50 and 86 compounds affected by processing time in SST, EDTA and P100 tubes, respectively, including many lipids. In contrast, 496 hydrophilic and 242 hydrophobic compounds differed by collection tube. Forty-five different chemical classes including alcohols, sugars, amino acids and prenol lipids were affected by the choice of blood collection tube. <i>Conclusion</i>: Our results suggest that the choice of blood collection tube has a significant effect on detected metabolites and their overall abundances. Perhaps surprisingly, variation in sample processing time has less of an effect compared to collection tube; however, a larger sample size is needed to confirm this.https://www.mdpi.com/2218-1989/8/4/88metabolomicslipidomicsclinicalcollection tubesbloodplasmaserum |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Charmion Cruickshank-Quinn Laura K. Zheng Kevin Quinn Russell Bowler Richard Reisdorph Nichole Reisdorph |
spellingShingle |
Charmion Cruickshank-Quinn Laura K. Zheng Kevin Quinn Russell Bowler Richard Reisdorph Nichole Reisdorph Impact of Blood Collection Tubes and Sample Handling Time on Serum and Plasma Metabolome and Lipidome Metabolites metabolomics lipidomics clinical collection tubes blood plasma serum |
author_facet |
Charmion Cruickshank-Quinn Laura K. Zheng Kevin Quinn Russell Bowler Richard Reisdorph Nichole Reisdorph |
author_sort |
Charmion Cruickshank-Quinn |
title |
Impact of Blood Collection Tubes and Sample Handling Time on Serum and Plasma Metabolome and Lipidome |
title_short |
Impact of Blood Collection Tubes and Sample Handling Time on Serum and Plasma Metabolome and Lipidome |
title_full |
Impact of Blood Collection Tubes and Sample Handling Time on Serum and Plasma Metabolome and Lipidome |
title_fullStr |
Impact of Blood Collection Tubes and Sample Handling Time on Serum and Plasma Metabolome and Lipidome |
title_full_unstemmed |
Impact of Blood Collection Tubes and Sample Handling Time on Serum and Plasma Metabolome and Lipidome |
title_sort |
impact of blood collection tubes and sample handling time on serum and plasma metabolome and lipidome |
publisher |
MDPI AG |
series |
Metabolites |
issn |
2218-1989 |
publishDate |
2018-12-01 |
description |
<i>Background</i>: Metabolomics is emerging as a valuable tool in clinical science. However, one major challenge in clinical metabolomics is the limited use of standardized guidelines for sample collection and handling. In this study, we conducted a pilot analysis of serum and plasma to determine the effects of processing time and collection tube on the metabolome. <i>Methods</i>: Blood was collected in 3 tubes: Vacutainer serum separator tube (SST, serum), EDTA (plasma) and P100 (plasma) and stored at 4 degrees for 0, 0.5, 1, 2, 4 and 24 h prior to centrifugation. Compounds were extracted using liquid-liquid extraction to obtain a hydrophilic and a hydrophobic fraction and analyzed using liquid chromatography mass spectrometry. Differences among the blood collection tubes and sample processing time were evaluated (ANOVA, Bonferroni FWER ≤ 0.05 and ANOVA, Benjamini Hochberg FDR ≤ 0.1, respectively). <i>Results</i>: Among the serum and plasma tubes 93.5% of compounds overlapped, 382 compounds were unique to serum and one compound was unique to plasma. There were 46, 50 and 86 compounds affected by processing time in SST, EDTA and P100 tubes, respectively, including many lipids. In contrast, 496 hydrophilic and 242 hydrophobic compounds differed by collection tube. Forty-five different chemical classes including alcohols, sugars, amino acids and prenol lipids were affected by the choice of blood collection tube. <i>Conclusion</i>: Our results suggest that the choice of blood collection tube has a significant effect on detected metabolites and their overall abundances. Perhaps surprisingly, variation in sample processing time has less of an effect compared to collection tube; however, a larger sample size is needed to confirm this. |
topic |
metabolomics lipidomics clinical collection tubes blood plasma serum |
url |
https://www.mdpi.com/2218-1989/8/4/88 |
work_keys_str_mv |
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