Peritoneal macrophages attenuate retinal ganglion cell survival and neurite outgrowth
Inflammation is a critical pathophysiological process that modulates neuronal survival in the central nervous system after disease or injury. However, the effects and mechanisms of macrophage activation on neuronal survival remain unclear. In the present study, we co-cultured adult Fischer rat retin...
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Wolters Kluwer Medknow Publications
2021-01-01
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doaj-740a1cf1b9894435afb0e21c4cfff4ef2020-12-02T13:12:34ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742021-01-011661121112610.4103/1673-5374.300462Peritoneal macrophages attenuate retinal ganglion cell survival and neurite outgrowthJia-Jian LiangYu-Fen LiuTsz Kin NgCi-Yan XuMingzhi ZhangChi Pui PangLing-Ping CenInflammation is a critical pathophysiological process that modulates neuronal survival in the central nervous system after disease or injury. However, the effects and mechanisms of macrophage activation on neuronal survival remain unclear. In the present study, we co-cultured adult Fischer rat retinas with primary peritoneal macrophages or zymosan-treated peritoneal macrophages for 7 days. Immunofluorescence analysis revealed that peritoneal macrophages reduced retinal ganglion cell survival and neurite outgrowth in the retinal explant compared with the control group. The addition of zymosan to peritoneal macrophages attenuated the survival and neurite outgrowth of retinal ganglion cells. Conditioned media from peritoneal macrophages also reduced retinal ganglion cell survival and neurite outgrowth. This result suggests that secretions from peritoneal macrophages mediate the inhibitory effects of these macrophages. In addition, increased inflammation- and oxidation-related gene expression may be related to the enhanced retinal ganglion cell degeneration caused by zymosan activation. In summary, this study revealed that primary rat peritoneal macrophages attenuated retinal ganglion cell survival and neurite outgrowth, and that macrophage activation further aggravated retinal ganglion cell degeneration. This study was approved by the Animal Ethics Committee of the Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Guangdong Province, China, on March 11, 2014 (approval no. EC20140311(2)-P01).http://www.nrronline.org/article.asp?issn=1673-5374;year=2021;volume=16;issue=6;spage=1121;epage=1126;aulast=in vitro; inflammation; macrophages; model; neurite outgrowth; optic nerve; retinal ganglion cells; survival |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jia-Jian Liang Yu-Fen Liu Tsz Kin Ng Ci-Yan Xu Mingzhi Zhang Chi Pui Pang Ling-Ping Cen |
spellingShingle |
Jia-Jian Liang Yu-Fen Liu Tsz Kin Ng Ci-Yan Xu Mingzhi Zhang Chi Pui Pang Ling-Ping Cen Peritoneal macrophages attenuate retinal ganglion cell survival and neurite outgrowth Neural Regeneration Research in vitro; inflammation; macrophages; model; neurite outgrowth; optic nerve; retinal ganglion cells; survival |
author_facet |
Jia-Jian Liang Yu-Fen Liu Tsz Kin Ng Ci-Yan Xu Mingzhi Zhang Chi Pui Pang Ling-Ping Cen |
author_sort |
Jia-Jian Liang |
title |
Peritoneal macrophages attenuate retinal ganglion cell survival and neurite outgrowth |
title_short |
Peritoneal macrophages attenuate retinal ganglion cell survival and neurite outgrowth |
title_full |
Peritoneal macrophages attenuate retinal ganglion cell survival and neurite outgrowth |
title_fullStr |
Peritoneal macrophages attenuate retinal ganglion cell survival and neurite outgrowth |
title_full_unstemmed |
Peritoneal macrophages attenuate retinal ganglion cell survival and neurite outgrowth |
title_sort |
peritoneal macrophages attenuate retinal ganglion cell survival and neurite outgrowth |
publisher |
Wolters Kluwer Medknow Publications |
series |
Neural Regeneration Research |
issn |
1673-5374 |
publishDate |
2021-01-01 |
description |
Inflammation is a critical pathophysiological process that modulates neuronal survival in the central nervous system after disease or injury. However, the effects and mechanisms of macrophage activation on neuronal survival remain unclear. In the present study, we co-cultured adult Fischer rat retinas with primary peritoneal macrophages or zymosan-treated peritoneal macrophages for 7 days. Immunofluorescence analysis revealed that peritoneal macrophages reduced retinal ganglion cell survival and neurite outgrowth in the retinal explant compared with the control group. The addition of zymosan to peritoneal macrophages attenuated the survival and neurite outgrowth of retinal ganglion cells. Conditioned media from peritoneal macrophages also reduced retinal ganglion cell survival and neurite outgrowth. This result suggests that secretions from peritoneal macrophages mediate the inhibitory effects of these macrophages. In addition, increased inflammation- and oxidation-related gene expression may be related to the enhanced retinal ganglion cell degeneration caused by zymosan activation. In summary, this study revealed that primary rat peritoneal macrophages attenuated retinal ganglion cell survival and neurite outgrowth, and that macrophage activation further aggravated retinal ganglion cell degeneration. This study was approved by the Animal Ethics Committee of the Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Guangdong Province, China, on March 11, 2014 (approval no. EC20140311(2)-P01). |
topic |
in vitro; inflammation; macrophages; model; neurite outgrowth; optic nerve; retinal ganglion cells; survival |
url |
http://www.nrronline.org/article.asp?issn=1673-5374;year=2021;volume=16;issue=6;spage=1121;epage=1126;aulast= |
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