Increased Chromogranin A Cell Density in the Large Intestine of Patients with Irritable Bowel Syndrome after Receiving Dietary Guidance
The large intestine contains five types of endocrine cells that regulate its functions by sensing its luminal contents and releasing specific hormones. Chromogranin A (CgA) is a common marker for the gastrointestinal endocrine cells, and it is abnormal in irritable bowel syndrome (IBS) patients. Mos...
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Online Access: | http://dx.doi.org/10.1155/2015/823897 |
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doaj-7413e1672336497695f137e7c1e247b72020-11-25T00:35:02ZengHindawi LimitedGastroenterology Research and Practice1687-61211687-630X2015-01-01201510.1155/2015/823897823897Increased Chromogranin A Cell Density in the Large Intestine of Patients with Irritable Bowel Syndrome after Receiving Dietary GuidanceTarek Mazzawi0Doris Gundersen1Trygve Hausken2Magdy El-Salhy3Division of Gastroenterology, Department of Medicine, Stord Hospital, 5416 Stord, NorwayDepartment of Research, Helse-Fonna, 5528 Haugesund, NorwayDivision of Gastroenterology, Department of Clinical Medicine, University of Bergen, 5020 Bergen, NorwayDivision of Gastroenterology, Department of Medicine, Stord Hospital, 5416 Stord, NorwayThe large intestine contains five types of endocrine cells that regulate its functions by sensing its luminal contents and releasing specific hormones. Chromogranin A (CgA) is a common marker for the gastrointestinal endocrine cells, and it is abnormal in irritable bowel syndrome (IBS) patients. Most IBS patients relate their symptoms to certain food elements. The present study investigated the effect of dietary guidance on the total endocrine cells of the large intestine as detected by CgA in 13 IBS patients. Thirteen control subjects were also included. Each patient received three sessions of dietary guidance. Colonoscopies were performed on controls and patients (at baseline and at 3–9 months after receiving guidance). Biopsy samples from the colon and rectum were immunostained for CgA and quantified by computerized image analysis. The densities of CgA cells in the total colon (mean ± SEM) among the controls and the IBS patients before and after receiving dietary guidance were 83.3±10.1, 38.6±3.7, and 64.7±4.2 cells/mm2, respectively (P=0.0004), and were unchanged in the rectum. In conclusion, the increase in CgA cell density after receiving dietary guidance may reflect a change in the densities of the large intestinal endocrine cells causing an improvement in the IBS symptoms.http://dx.doi.org/10.1155/2015/823897 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tarek Mazzawi Doris Gundersen Trygve Hausken Magdy El-Salhy |
spellingShingle |
Tarek Mazzawi Doris Gundersen Trygve Hausken Magdy El-Salhy Increased Chromogranin A Cell Density in the Large Intestine of Patients with Irritable Bowel Syndrome after Receiving Dietary Guidance Gastroenterology Research and Practice |
author_facet |
Tarek Mazzawi Doris Gundersen Trygve Hausken Magdy El-Salhy |
author_sort |
Tarek Mazzawi |
title |
Increased Chromogranin A Cell Density in the Large Intestine of Patients with Irritable Bowel Syndrome after Receiving Dietary Guidance |
title_short |
Increased Chromogranin A Cell Density in the Large Intestine of Patients with Irritable Bowel Syndrome after Receiving Dietary Guidance |
title_full |
Increased Chromogranin A Cell Density in the Large Intestine of Patients with Irritable Bowel Syndrome after Receiving Dietary Guidance |
title_fullStr |
Increased Chromogranin A Cell Density in the Large Intestine of Patients with Irritable Bowel Syndrome after Receiving Dietary Guidance |
title_full_unstemmed |
Increased Chromogranin A Cell Density in the Large Intestine of Patients with Irritable Bowel Syndrome after Receiving Dietary Guidance |
title_sort |
increased chromogranin a cell density in the large intestine of patients with irritable bowel syndrome after receiving dietary guidance |
publisher |
Hindawi Limited |
series |
Gastroenterology Research and Practice |
issn |
1687-6121 1687-630X |
publishDate |
2015-01-01 |
description |
The large intestine contains five types of endocrine cells that regulate its functions by sensing its luminal contents and releasing specific hormones. Chromogranin A (CgA) is a common marker for the gastrointestinal endocrine cells, and it is abnormal in irritable bowel syndrome (IBS) patients. Most IBS patients relate their symptoms to certain food elements. The present study investigated the effect of dietary guidance on the total endocrine cells of the large intestine as detected by CgA in 13 IBS patients. Thirteen control subjects were also included. Each patient received three sessions of dietary guidance. Colonoscopies were performed on controls and patients (at baseline and at 3–9 months after receiving guidance). Biopsy samples from the colon and rectum were immunostained for CgA and quantified by computerized image analysis. The densities of CgA cells in the total colon (mean ± SEM) among the controls and the IBS patients before and after receiving dietary guidance were 83.3±10.1, 38.6±3.7, and 64.7±4.2 cells/mm2, respectively (P=0.0004), and were unchanged in the rectum. In conclusion, the increase in CgA cell density after receiving dietary guidance may reflect a change in the densities of the large intestinal endocrine cells causing an improvement in the IBS symptoms. |
url |
http://dx.doi.org/10.1155/2015/823897 |
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