Rapid MALDI-TOF mass spectrometry strain typing during a large outbreak of Shiga-Toxigenic Escherichia coli.

<h4>Background</h4>In 2011 northern Germany experienced a large outbreak of Shiga-Toxigenic Escherichia coli O104:H4. The large amount of samples sent to microbiology laboratories for epidemiological assessment highlighted the importance of fast and inexpensive typing procedures. We have...

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Main Authors: Martin Christner, Maria Trusch, Holger Rohde, Marcel Kwiatkowski, Hartmut Schlüter, Manuel Wolters, Martin Aepfelbacher, Moritz Hentschke
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25003758/?tool=EBI
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spelling doaj-74157a1548d746fab3bd06e451d394862021-06-19T04:56:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0197e10192410.1371/journal.pone.0101924Rapid MALDI-TOF mass spectrometry strain typing during a large outbreak of Shiga-Toxigenic Escherichia coli.Martin ChristnerMaria TruschHolger RohdeMarcel KwiatkowskiHartmut SchlüterManuel WoltersMartin AepfelbacherMoritz Hentschke<h4>Background</h4>In 2011 northern Germany experienced a large outbreak of Shiga-Toxigenic Escherichia coli O104:H4. The large amount of samples sent to microbiology laboratories for epidemiological assessment highlighted the importance of fast and inexpensive typing procedures. We have therefore evaluated the applicability of a MALDI-TOF mass spectrometry based strategy for outbreak strain identification.<h4>Methods</h4>Specific peaks in the outbreak strain's spectrum were identified by comparative analysis of archived pre-outbreak spectra that had been acquired for routine species-level identification. Proteins underlying these discriminatory peaks were identified by liquid chromatography tandem mass spectrometry and validated against publicly available databases. The resulting typing scheme was evaluated against PCR genotyping with 294 E. coli isolates from clinical samples collected during the outbreak.<h4>Results</h4>Comparative spectrum analysis revealed two characteristic peaks at m/z 6711 and m/z 10883. The underlying proteins were found to be of low prevalence among genome sequenced E. coli strains. Marker peak detection correctly classified 292 of 293 study isolates, including all 104 outbreak isolates.<h4>Conclusions</h4>MALDI-TOF mass spectrometry allowed for reliable outbreak strain identification during a large outbreak of Shiga-Toxigenic E. coli. The applied typing strategy could probably be adapted to other typing tasks and might facilitate epidemiological surveys as part of the routine pathogen identification workflow.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25003758/?tool=EBI
collection DOAJ
language English
format Article
sources DOAJ
author Martin Christner
Maria Trusch
Holger Rohde
Marcel Kwiatkowski
Hartmut Schlüter
Manuel Wolters
Martin Aepfelbacher
Moritz Hentschke
spellingShingle Martin Christner
Maria Trusch
Holger Rohde
Marcel Kwiatkowski
Hartmut Schlüter
Manuel Wolters
Martin Aepfelbacher
Moritz Hentschke
Rapid MALDI-TOF mass spectrometry strain typing during a large outbreak of Shiga-Toxigenic Escherichia coli.
PLoS ONE
author_facet Martin Christner
Maria Trusch
Holger Rohde
Marcel Kwiatkowski
Hartmut Schlüter
Manuel Wolters
Martin Aepfelbacher
Moritz Hentschke
author_sort Martin Christner
title Rapid MALDI-TOF mass spectrometry strain typing during a large outbreak of Shiga-Toxigenic Escherichia coli.
title_short Rapid MALDI-TOF mass spectrometry strain typing during a large outbreak of Shiga-Toxigenic Escherichia coli.
title_full Rapid MALDI-TOF mass spectrometry strain typing during a large outbreak of Shiga-Toxigenic Escherichia coli.
title_fullStr Rapid MALDI-TOF mass spectrometry strain typing during a large outbreak of Shiga-Toxigenic Escherichia coli.
title_full_unstemmed Rapid MALDI-TOF mass spectrometry strain typing during a large outbreak of Shiga-Toxigenic Escherichia coli.
title_sort rapid maldi-tof mass spectrometry strain typing during a large outbreak of shiga-toxigenic escherichia coli.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2014-01-01
description <h4>Background</h4>In 2011 northern Germany experienced a large outbreak of Shiga-Toxigenic Escherichia coli O104:H4. The large amount of samples sent to microbiology laboratories for epidemiological assessment highlighted the importance of fast and inexpensive typing procedures. We have therefore evaluated the applicability of a MALDI-TOF mass spectrometry based strategy for outbreak strain identification.<h4>Methods</h4>Specific peaks in the outbreak strain's spectrum were identified by comparative analysis of archived pre-outbreak spectra that had been acquired for routine species-level identification. Proteins underlying these discriminatory peaks were identified by liquid chromatography tandem mass spectrometry and validated against publicly available databases. The resulting typing scheme was evaluated against PCR genotyping with 294 E. coli isolates from clinical samples collected during the outbreak.<h4>Results</h4>Comparative spectrum analysis revealed two characteristic peaks at m/z 6711 and m/z 10883. The underlying proteins were found to be of low prevalence among genome sequenced E. coli strains. Marker peak detection correctly classified 292 of 293 study isolates, including all 104 outbreak isolates.<h4>Conclusions</h4>MALDI-TOF mass spectrometry allowed for reliable outbreak strain identification during a large outbreak of Shiga-Toxigenic E. coli. The applied typing strategy could probably be adapted to other typing tasks and might facilitate epidemiological surveys as part of the routine pathogen identification workflow.
url https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25003758/?tool=EBI
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