Detection of Quorum Sensing Signals in Gram-Negative Bacteria by Using Reporter Strain CV026
Quorum sensing signals are referred to as acylated homoserine lactones (AHL) that are mainly found in Gram-negative bacteria. It implies the ability of certain bacteria of producing different AHL molecules. The bacteria Pseudomonas aureofaciens and Xenorhabdus nematophila were cultured in Luria-Bert...
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University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca
2010-12-01
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doaj-741796b03c9947cf956e92515e1b54a72020-11-25T01:54:29ZengUniversity of Agricultural Sciences and Veterinary Medicine, Cluj-NapocaNotulae Scientia Biologicae2067-32052067-32642010-12-0124727510.15835/nsb2448635444Detection of Quorum Sensing Signals in Gram-Negative Bacteria by Using Reporter Strain CV026Ahmad Humayan KABIR0Anindya GHOSH ROY1Mohammad FIROZ ALAM2Rafiul ISLAM3Flinders University, School of Biological Sciences, SA 5042Max Planck Institute for Developmental Biology, D-72076 T�bingenUniversity of Rajshahi, Department of Botany, Rajshahi 6205, BangladeshUniversity of Rajshahi, Department of Botany, Rajshahi 6205, BangladeshQuorum sensing signals are referred to as acylated homoserine lactones (AHL) that are mainly found in Gram-negative bacteria. It implies the ability of certain bacteria of producing different AHL molecules. The bacteria Pseudomonas aureofaciens and Xenorhabdus nematophila were cultured in Luria-Bertani (LB10) media and CV026 was used as a reporter strain to detect the presence of AHLs produced by the cultured bacteria. In this study, the reporter strain has revealed the quorum sensing ability of P. aureofaciens and X. nematophila by producing the purple pigment violacein in the supply of external AHLs molecules. Thin layer chromatography (TLC) bioassay having four controls was conducted to detect specific AHL molecule supplied by P. aureofaciens and X. nematophila. The specific AHL molecule was observed to be migrated according to their polarity on the TLC plate.http://www.notulaebiologicae.ro/index.php/nsb/article/view/4863 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ahmad Humayan KABIR Anindya GHOSH ROY Mohammad FIROZ ALAM Rafiul ISLAM |
spellingShingle |
Ahmad Humayan KABIR Anindya GHOSH ROY Mohammad FIROZ ALAM Rafiul ISLAM Detection of Quorum Sensing Signals in Gram-Negative Bacteria by Using Reporter Strain CV026 Notulae Scientia Biologicae |
author_facet |
Ahmad Humayan KABIR Anindya GHOSH ROY Mohammad FIROZ ALAM Rafiul ISLAM |
author_sort |
Ahmad Humayan KABIR |
title |
Detection of Quorum Sensing Signals in Gram-Negative Bacteria by Using Reporter Strain CV026 |
title_short |
Detection of Quorum Sensing Signals in Gram-Negative Bacteria by Using Reporter Strain CV026 |
title_full |
Detection of Quorum Sensing Signals in Gram-Negative Bacteria by Using Reporter Strain CV026 |
title_fullStr |
Detection of Quorum Sensing Signals in Gram-Negative Bacteria by Using Reporter Strain CV026 |
title_full_unstemmed |
Detection of Quorum Sensing Signals in Gram-Negative Bacteria by Using Reporter Strain CV026 |
title_sort |
detection of quorum sensing signals in gram-negative bacteria by using reporter strain cv026 |
publisher |
University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca |
series |
Notulae Scientia Biologicae |
issn |
2067-3205 2067-3264 |
publishDate |
2010-12-01 |
description |
Quorum sensing signals are referred to as acylated homoserine lactones (AHL) that are mainly found in Gram-negative bacteria. It implies the ability of certain bacteria of producing different AHL molecules. The bacteria Pseudomonas aureofaciens and Xenorhabdus nematophila were cultured in Luria-Bertani (LB10) media and CV026 was used as a reporter strain to detect the presence of AHLs produced by the cultured bacteria. In this study, the reporter strain has revealed the quorum sensing ability of P. aureofaciens and X. nematophila by producing the purple pigment violacein in the supply of external AHLs molecules. Thin layer chromatography (TLC) bioassay having four controls was conducted to detect specific AHL molecule supplied by P. aureofaciens and X. nematophila. The specific AHL molecule was observed to be migrated according to their polarity on the TLC plate. |
url |
http://www.notulaebiologicae.ro/index.php/nsb/article/view/4863 |
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