Increasing CRISPR Efficiency and Measuring Its Specificity in HSPCs Using a Clinically Relevant System
Genome editing of human cluster of differentiation 34+ (CD34+) hematopoietic stem and progenitor cells (HSPCs) holds great therapeutic potential. This study aimed to optimize on-target, ex vivo genome editing using the CRISPR-Cas9 system in CD34+ HSPCs and to create a clear workflow for precise iden...
Main Authors: | Jenny Shapiro, Ortal Iancu, Ashley M. Jacobi, Matthew S. McNeill, Rolf Turk, Garrett R. Rettig, Ido Amit, Adi Tovin-Recht, Zohar Yakhini, Mark A. Behlke, Ayal Hendel |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2020-06-01
|
Series: | Molecular Therapy: Methods & Clinical Development |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2329050120300875 |
Similar Items
-
Induction of AML Preleukemic Fusion Genes in HSPCs and DNA Damage Response in Preleukemic Fusion Gene Positive Samples
by: Pavol Kosik, et al.
Published: (2021-03-01) -
Vpx mediated degradation of SAMHD1 has only a very limited effect on lentiviral transduction rate in ex vivo cultured HSPCs
by: Duo Li, et al.
Published: (2015-09-01) -
Advantages of adipose tissue stem cells over CD34+ mobilization to decrease hepatic fibrosis in Wistar rats
by: Marcela M. De Luna-Saldivar, et al.
Published: (2019-07-01) -
HSPCs display within-family homogeneity in differentiation and proliferation despite population heterogeneity
by: Tamar Tak, et al.
Published: (2021-05-01) -
Rapid and Reproducible Differentiation of Hematopoietic and T Cell Progenitors From Pluripotent Stem Cells
by: Léa Flippe, et al.
Published: (2020-10-01)