LncRNA TP73-AS1 Activates TGF-β1 to Promote the Migration and Invasion of Colorectal Cancer Cell

• Main Authors: Li M, Jin Y, Li Y
• Format: Article
• Language: English
• Published: Dove Medical Press 2019-12-01
• Series: Cancer Management and Research
• Subjects: colorectal cancer; lncrna tp73-as1; tgf-β1; prognosis
• Online Access: https://www.dovepress.com/lncrna-tp73-as1-activates-tgf-beta1-to-promote-the-migration-and-invas-peer-reviewed-article-CMAR

Mingli Li,1 Yuanyuan Jin,2 Yuanzhi Li3 1Department of Chinese traditional medicine, Jining University, Qufu City, Shandong Province, 273155, People’s Republic of China; 2The First People’s Hospital of Yuzhong County, Lanzhou City, Gansu Province 730100, People’s Republic of China; 3Department of Anorectal Hemorrhoid Fistula, The Affiliated Hospital of Southwest Medical University, Luzhou City, Sichuan Province 646000, People’s Republic of ChinaCorrespondence: Yuanzhi LiDepartment of Anorectal Hemorrhoid Fistula, The Affiliated Hospital of Southwest Medical University, No. 25 Taiping Street, Luzhou City, Sichuan Province 646000, People’s Republic of ChinaTel [phone]Email [e-mail]: LncRNA TP73-AS1 has been demonstrated to promote the developments of several types of human cancer. However, its role in colorectal cancer (CRC) is unknown.Methods: All CRC patients (n=70, 40 males and 30 females, 38 to 66 years’ old, 52.1 ± 5.3 years’ old) in this study were enrolled in the Affiliated Hospital of Southwest Medical University from July 2012 to January 2014. Cells, vectors, and transient transfections, RT-qPCR, western-blotting, as well as measurements of cell migration and invasion abilities were carried out during the research.Results: In the present study, we found that TP73-AS1 was upregulated in CRC tissues compared with adjacent non-CRC tissues in CRC patients. Upregulation of TP73-AS1 was closely correlated with poor prognosis. TGF-β1 was also upregulated in CRC tissues and positive correlated with TP73-AS1. TP73-AS1 overexpression caused upregulated TGF-β1 in CRC cells, while TGF-β1 overexpression showed no significant effect on TP73-AS1. TP73-AS1 and TGF-β1 overexpressions caused enhanced migration and invasion of CRC cells. TGF-β inhibitor treatment caused suppressed migration and invasion of CRC cells and attenuated effects of TP73-AS1 and TGF-β1 overexpression.Conclusion: Therefore, TP73-AS1 may inactivate TGF-β1 to inhibit the migration and invasion of CRC cells.Keywords: colorectal cancer, lncRNA TP73-AS1, TGF-β1, prognosis