In Vivo Formation of Stable Hyaline Cartilage by Naïve Human Bone Marrow Stromal Cells with Modified Fibrin Microbeads
Abstract Osteoarthritic and other types of articular cartilage defects never heal on their own. Medicinal and surgical approaches are often ineffective, and the supply of autologous chondrocytes for tissue engineering is very limited. Bone marrow stromal cells (BMSCs, also known as bone marrow‐deriv...
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doaj-744217abc0694c649aef83e5a7dca4522020-11-24T22:19:08ZengWileyStem Cells Translational Medicine2157-65642157-65802019-06-018658659210.1002/sctm.18-0129In Vivo Formation of Stable Hyaline Cartilage by Naïve Human Bone Marrow Stromal Cells with Modified Fibrin MicrobeadsSergei A. Kuznetsov0Astar Hailu‐Lazmi1Natasha Cherman2Luis F. deCastro3Pamela G. Robey4Raphael Gorodetsky5Department of Health and Human Services, Skeletal Biology Section, National Institute of Dental and Craniofacial Research National Institutes of Health Bethesda Maryland USABiotechnology and Radiobiology Laboratory Hadassah ‐ Hebrew University Medical Center, Sharett Institute of Oncology Jerusalem IsraelDepartment of Health and Human Services, Skeletal Biology Section, National Institute of Dental and Craniofacial Research National Institutes of Health Bethesda Maryland USADepartment of Health and Human Services, Skeletal Biology Section, National Institute of Dental and Craniofacial Research National Institutes of Health Bethesda Maryland USADepartment of Health and Human Services, Skeletal Biology Section, National Institute of Dental and Craniofacial Research National Institutes of Health Bethesda Maryland USABiotechnology and Radiobiology Laboratory Hadassah ‐ Hebrew University Medical Center, Sharett Institute of Oncology Jerusalem IsraelAbstract Osteoarthritic and other types of articular cartilage defects never heal on their own. Medicinal and surgical approaches are often ineffective, and the supply of autologous chondrocytes for tissue engineering is very limited. Bone marrow stromal cells (BMSCs, also known as bone marrow‐derived mesenchymal stem cells) have been suggested as an adequate cell source for cartilage reconstruction. However, the majority of studies employing BMSCs for cartilage tissue engineering have used BMSCs predifferentiated into cartilage prior to implantation. This strategy has failed to achieve formation of stable, hyaline‐like cartilage, resistant to hypertrophy in vivo. We hypothesized that in vitro predifferentiation of BMSCs is not necessary when cells are combined with an adequate scaffold that supports the formation of stable cartilage in vivo. In this study, naïve (undifferentiated) human BMSCs were attached to dehydrothermally crosslinked stable fibrin microbeads (FMBs) without and with other scaffolds and implanted subcutaneously into immunocompromised mice. Optimal formation of abundant, hypertrophy‐resistant, ectopic hyaline‐like cartilage was achieved when BMSCs were attached to FMBs covalently coated with hyaluronic acid. The cartilage that was formed was of human origin and was stable for at least 28 weeks in vivo. Stem Cells Translational Medicine 2019;8:586–592https://doi.org/10.1002/sctm.18-0129Bone marrow stromal cellsCartilage formationIn vivo implantationFibrin microbeads |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sergei A. Kuznetsov Astar Hailu‐Lazmi Natasha Cherman Luis F. deCastro Pamela G. Robey Raphael Gorodetsky |
spellingShingle |
Sergei A. Kuznetsov Astar Hailu‐Lazmi Natasha Cherman Luis F. deCastro Pamela G. Robey Raphael Gorodetsky In Vivo Formation of Stable Hyaline Cartilage by Naïve Human Bone Marrow Stromal Cells with Modified Fibrin Microbeads Stem Cells Translational Medicine Bone marrow stromal cells Cartilage formation In vivo implantation Fibrin microbeads |
author_facet |
Sergei A. Kuznetsov Astar Hailu‐Lazmi Natasha Cherman Luis F. deCastro Pamela G. Robey Raphael Gorodetsky |
author_sort |
Sergei A. Kuznetsov |
title |
In Vivo Formation of Stable Hyaline Cartilage by Naïve Human Bone Marrow Stromal Cells with Modified Fibrin Microbeads |
title_short |
In Vivo Formation of Stable Hyaline Cartilage by Naïve Human Bone Marrow Stromal Cells with Modified Fibrin Microbeads |
title_full |
In Vivo Formation of Stable Hyaline Cartilage by Naïve Human Bone Marrow Stromal Cells with Modified Fibrin Microbeads |
title_fullStr |
In Vivo Formation of Stable Hyaline Cartilage by Naïve Human Bone Marrow Stromal Cells with Modified Fibrin Microbeads |
title_full_unstemmed |
In Vivo Formation of Stable Hyaline Cartilage by Naïve Human Bone Marrow Stromal Cells with Modified Fibrin Microbeads |
title_sort |
in vivo formation of stable hyaline cartilage by naïve human bone marrow stromal cells with modified fibrin microbeads |
publisher |
Wiley |
series |
Stem Cells Translational Medicine |
issn |
2157-6564 2157-6580 |
publishDate |
2019-06-01 |
description |
Abstract Osteoarthritic and other types of articular cartilage defects never heal on their own. Medicinal and surgical approaches are often ineffective, and the supply of autologous chondrocytes for tissue engineering is very limited. Bone marrow stromal cells (BMSCs, also known as bone marrow‐derived mesenchymal stem cells) have been suggested as an adequate cell source for cartilage reconstruction. However, the majority of studies employing BMSCs for cartilage tissue engineering have used BMSCs predifferentiated into cartilage prior to implantation. This strategy has failed to achieve formation of stable, hyaline‐like cartilage, resistant to hypertrophy in vivo. We hypothesized that in vitro predifferentiation of BMSCs is not necessary when cells are combined with an adequate scaffold that supports the formation of stable cartilage in vivo. In this study, naïve (undifferentiated) human BMSCs were attached to dehydrothermally crosslinked stable fibrin microbeads (FMBs) without and with other scaffolds and implanted subcutaneously into immunocompromised mice. Optimal formation of abundant, hypertrophy‐resistant, ectopic hyaline‐like cartilage was achieved when BMSCs were attached to FMBs covalently coated with hyaluronic acid. The cartilage that was formed was of human origin and was stable for at least 28 weeks in vivo. Stem Cells Translational Medicine 2019;8:586–592 |
topic |
Bone marrow stromal cells Cartilage formation In vivo implantation Fibrin microbeads |
url |
https://doi.org/10.1002/sctm.18-0129 |
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