Prevalence, Genetic Diversity, and Temporary Shifts of Inducible Clindamycin Resistance Staphylococcus aureus Clones in Tehran, Iran: A Molecular–Epidemiological Analysis From 2013 to 2018
The prevalence of Staphylococcus aureus as an aggressive pathogen resistant to multiple antibiotics causing nosocomial and community-acquired infections is increasing with limited therapeutic options. Macrolide-lincosamide streptogramin B (MLSB) family of antibiotics represents an important alternat...
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Frontiers Media S.A.
2020-04-01
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Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.00663/full |
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doaj-74642795f2de44b4ba27ed97fd608218 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mehdi Goudarzi Nobumichi Kobayashi Masoud Dadashi Roman Pantůček Mohammad Javad Nasiri Maryam Fazeli Ramin Pouriran Hossein Goudarzi Mirmohammad Miri Anahita Amirpour Sima Sadat Seyedjavadi |
spellingShingle |
Mehdi Goudarzi Nobumichi Kobayashi Masoud Dadashi Roman Pantůček Mohammad Javad Nasiri Maryam Fazeli Ramin Pouriran Hossein Goudarzi Mirmohammad Miri Anahita Amirpour Sima Sadat Seyedjavadi Prevalence, Genetic Diversity, and Temporary Shifts of Inducible Clindamycin Resistance Staphylococcus aureus Clones in Tehran, Iran: A Molecular–Epidemiological Analysis From 2013 to 2018 Frontiers in Microbiology methicillin-resistant S. aureus methicillin-susceptible S. aureus inducible resistance staphylocoagulase SCCmec agr allotype |
author_facet |
Mehdi Goudarzi Nobumichi Kobayashi Masoud Dadashi Roman Pantůček Mohammad Javad Nasiri Maryam Fazeli Ramin Pouriran Hossein Goudarzi Mirmohammad Miri Anahita Amirpour Sima Sadat Seyedjavadi |
author_sort |
Mehdi Goudarzi |
title |
Prevalence, Genetic Diversity, and Temporary Shifts of Inducible Clindamycin Resistance Staphylococcus aureus Clones in Tehran, Iran: A Molecular–Epidemiological Analysis From 2013 to 2018 |
title_short |
Prevalence, Genetic Diversity, and Temporary Shifts of Inducible Clindamycin Resistance Staphylococcus aureus Clones in Tehran, Iran: A Molecular–Epidemiological Analysis From 2013 to 2018 |
title_full |
Prevalence, Genetic Diversity, and Temporary Shifts of Inducible Clindamycin Resistance Staphylococcus aureus Clones in Tehran, Iran: A Molecular–Epidemiological Analysis From 2013 to 2018 |
title_fullStr |
Prevalence, Genetic Diversity, and Temporary Shifts of Inducible Clindamycin Resistance Staphylococcus aureus Clones in Tehran, Iran: A Molecular–Epidemiological Analysis From 2013 to 2018 |
title_full_unstemmed |
Prevalence, Genetic Diversity, and Temporary Shifts of Inducible Clindamycin Resistance Staphylococcus aureus Clones in Tehran, Iran: A Molecular–Epidemiological Analysis From 2013 to 2018 |
title_sort |
prevalence, genetic diversity, and temporary shifts of inducible clindamycin resistance staphylococcus aureus clones in tehran, iran: a molecular–epidemiological analysis from 2013 to 2018 |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2020-04-01 |
description |
The prevalence of Staphylococcus aureus as an aggressive pathogen resistant to multiple antibiotics causing nosocomial and community-acquired infections is increasing with limited therapeutic options. Macrolide-lincosamide streptogramin B (MLSB) family of antibiotics represents an important alternative therapy for staphylococcal infections. This study was conducted over a period of five years from August 2013 to July 2018 to investigate the prevalence and molecular epidemiology in Iran of inducible resistance in S. aureus. In the current study, 126 inducible methicillin-resistant S. aureus (MRSA) (n = 106) and methicillin-sensitive S. aureus (MSSA) (n = 20) isolates were characterized by in vitro susceptibility analysis, resistance and virulence encoding gene distribution, phenotypic and genotypic analysis of biofilm formation, prophage typing, S. aureus protein A locus (spa) typing, staphylocoagulase (SC) typing, staphylococcal cassette chromosome mec (SCCmec) typing, and multilocus sequence typing. Of the 126 isolates, 76 (60.3%) were classified as hospital onset, and 50 (39.7%) were classified as community onset (CO). Biofilm formation was observed in 97 strains (77%). A total of 14 sequence types (STs), 26 spa types, 7 coagulase types, 9 prophage types, 3 agr types (no agr IV), and 9 clonal complexes (CCs) were identified in this study. The prevalence of the inducible MLSB (iMLSB) S. aureus increased from 7.5% (25/335) to 21.7% (38/175) during the study period. The iMLSB MRSA isolates were distributed in nine CCs, whereas the MSSA isolates were less diverse, which mainly belonged to CC22 (7.95%) and CC30 (7.95%). High-level mupirocin-resistant strains belonged to ST85-SCCmec IV/t008 (n = 4), ST5-SCCmec IV/t002 (n = 4), ST239-SCCmec III/t631 (n = 2), and ST8-SCCmec IV/t064 (n = 2) clones, whereas low-level mupirocin-resistant strains belonged to ST15-SCCmec IV/t084 (n = 5), ST239-SCCmec III/t860 (n = 3), and ST22-SCCmec IV/t790 (n = 3) clones. All the fusidic acid–resistant iMLSB isolates were MRSA and belonged to ST15-SCCmec IV/t084 (n = 2), ST239-SCCmec III/t030 (n = 2), ST1-SCCmec V/t6811 (n = 1), ST80-SCCmec IV/t044 (n = 1), and ST59-SCCmec IV/t437 (n = 1). The CC22 that was predominant in 2013–2014 (36% of the isolates) had almost disappeared in 2017–2018, being replaced by the CC8, which represented 39.5% of the 2017–2018 isolates. This is the first description of temporal shifts of iMLSB S. aureus isolates in Iran that identifies predominant clones and treatment options for iMLSB S. aureus–related infections. |
topic |
methicillin-resistant S. aureus methicillin-susceptible S. aureus inducible resistance staphylocoagulase SCCmec agr allotype |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2020.00663/full |
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doaj-74642795f2de44b4ba27ed97fd6082182020-11-25T02:11:13ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-04-011110.3389/fmicb.2020.00663493144Prevalence, Genetic Diversity, and Temporary Shifts of Inducible Clindamycin Resistance Staphylococcus aureus Clones in Tehran, Iran: A Molecular–Epidemiological Analysis From 2013 to 2018Mehdi Goudarzi0Nobumichi Kobayashi1Masoud Dadashi2Roman Pantůček3Mohammad Javad Nasiri4Maryam Fazeli5Ramin Pouriran6Hossein Goudarzi7Mirmohammad Miri8Anahita Amirpour9Sima Sadat Seyedjavadi10Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Hygiene, School of Medicine, Sapporo Medical University, Sapporo, JapanDepartment of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, IranDepartment of Experimental Biology, Faculty of Science, Masaryk University, Brno, CzechiaDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Virology, Pasteur Institute of Iran, Tehran, IranSchool of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Critical Care and Anesthesiology, Imam Hossein Hospital, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Internal Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Mycology, Pasteur Institute of Iran, Tehran, IranThe prevalence of Staphylococcus aureus as an aggressive pathogen resistant to multiple antibiotics causing nosocomial and community-acquired infections is increasing with limited therapeutic options. Macrolide-lincosamide streptogramin B (MLSB) family of antibiotics represents an important alternative therapy for staphylococcal infections. This study was conducted over a period of five years from August 2013 to July 2018 to investigate the prevalence and molecular epidemiology in Iran of inducible resistance in S. aureus. In the current study, 126 inducible methicillin-resistant S. aureus (MRSA) (n = 106) and methicillin-sensitive S. aureus (MSSA) (n = 20) isolates were characterized by in vitro susceptibility analysis, resistance and virulence encoding gene distribution, phenotypic and genotypic analysis of biofilm formation, prophage typing, S. aureus protein A locus (spa) typing, staphylocoagulase (SC) typing, staphylococcal cassette chromosome mec (SCCmec) typing, and multilocus sequence typing. Of the 126 isolates, 76 (60.3%) were classified as hospital onset, and 50 (39.7%) were classified as community onset (CO). Biofilm formation was observed in 97 strains (77%). A total of 14 sequence types (STs), 26 spa types, 7 coagulase types, 9 prophage types, 3 agr types (no agr IV), and 9 clonal complexes (CCs) were identified in this study. The prevalence of the inducible MLSB (iMLSB) S. aureus increased from 7.5% (25/335) to 21.7% (38/175) during the study period. The iMLSB MRSA isolates were distributed in nine CCs, whereas the MSSA isolates were less diverse, which mainly belonged to CC22 (7.95%) and CC30 (7.95%). High-level mupirocin-resistant strains belonged to ST85-SCCmec IV/t008 (n = 4), ST5-SCCmec IV/t002 (n = 4), ST239-SCCmec III/t631 (n = 2), and ST8-SCCmec IV/t064 (n = 2) clones, whereas low-level mupirocin-resistant strains belonged to ST15-SCCmec IV/t084 (n = 5), ST239-SCCmec III/t860 (n = 3), and ST22-SCCmec IV/t790 (n = 3) clones. All the fusidic acid–resistant iMLSB isolates were MRSA and belonged to ST15-SCCmec IV/t084 (n = 2), ST239-SCCmec III/t030 (n = 2), ST1-SCCmec V/t6811 (n = 1), ST80-SCCmec IV/t044 (n = 1), and ST59-SCCmec IV/t437 (n = 1). The CC22 that was predominant in 2013–2014 (36% of the isolates) had almost disappeared in 2017–2018, being replaced by the CC8, which represented 39.5% of the 2017–2018 isolates. This is the first description of temporal shifts of iMLSB S. aureus isolates in Iran that identifies predominant clones and treatment options for iMLSB S. aureus–related infections.https://www.frontiersin.org/article/10.3389/fmicb.2020.00663/fullmethicillin-resistant S. aureusmethicillin-susceptible S. aureusinducible resistancestaphylocoagulaseSCCmecagr allotype |