Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy

Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy

Nanosecond imaging techniques, such as fluorescence lifetime imaging microscopy (FLIM), are limited by low efficiency of current detectors. Here, the authors implement an electro-optic approach using Pockels cells for wide-field image gating and demonstrate high throughput FLIM on standard camera se...

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Bibliographic Details
Main Authors: Adam J. Bowman, Brannon B. Klopfer, Thomas Juffmann, Mark A. Kasevich
Format: Article
Language:English
Published: Nature Publishing Group 2019-10-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-12535-5
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spelling doaj-7476a65533a64efdaa7ac34d856725f62021-05-11T12:35:17ZengNature Publishing GroupNature Communications2041-17232019-10-011011810.1038/s41467-019-12535-5Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopyAdam J. Bowman0Brannon B. Klopfer1Thomas Juffmann2Mark A. Kasevich3Physics Department, Stanford UniversityPhysics Department, Stanford UniversityFaculty of Physics, University of ViennaPhysics Department, Stanford UniversityNanosecond imaging techniques, such as fluorescence lifetime imaging microscopy (FLIM), are limited by low efficiency of current detectors. Here, the authors implement an electro-optic approach using Pockels cells for wide-field image gating and demonstrate high throughput FLIM on standard camera sensors.https://doi.org/10.1038/s41467-019-12535-5
collection DOAJ
language English
format Article
sources DOAJ
author Adam J. Bowman
Brannon B. Klopfer
Thomas Juffmann
Mark A. Kasevich
spellingShingle Adam J. Bowman
Brannon B. Klopfer
Thomas Juffmann
Mark A. Kasevich
Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy
Nature Communications
author_facet Adam J. Bowman
Brannon B. Klopfer
Thomas Juffmann
Mark A. Kasevich
author_sort Adam J. Bowman
title Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy
title_short Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy
title_full Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy
title_fullStr Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy
title_full_unstemmed Electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy
title_sort electro-optic imaging enables efficient wide-field fluorescence lifetime microscopy
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2019-10-01
description Nanosecond imaging techniques, such as fluorescence lifetime imaging microscopy (FLIM), are limited by low efficiency of current detectors. Here, the authors implement an electro-optic approach using Pockels cells for wide-field image gating and demonstrate high throughput FLIM on standard camera sensors.
url https://doi.org/10.1038/s41467-019-12535-5
work_keys_str_mv AT adamjbowman electroopticimagingenablesefficientwidefieldfluorescencelifetimemicroscopy
AT brannonbklopfer electroopticimagingenablesefficientwidefieldfluorescencelifetimemicroscopy
AT thomasjuffmann electroopticimagingenablesefficientwidefieldfluorescencelifetimemicroscopy
AT markakasevich electroopticimagingenablesefficientwidefieldfluorescencelifetimemicroscopy
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