Establishing a Split Luciferase Assay for Proteinkinase G (PKG) Interaction Studies

Establishing a Split Luciferase Assay for Proteinkinase G (PKG) Interaction Studies

Nitric oxide (NO/cyclic guanosine monophosphate (cGMP)-regulated cellular mechanisms are involved in a variety of (patho-) physiological processes. One of the main effector molecules in this system, proteinkinase G (PKG), serves as a molecular switch by phosphorylating different target proteins and...

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Bibliographic Details
Main Authors: Andrea Schramm, Philip Mueller-Thuemen, Timo Littmann, Manuela Harloff, Takeaki Ozawa, Jens Schlossmann
Format: Article
Language:English
Published: MDPI AG 2018-04-01
Series:International Journal of Molecular Sciences
Subjects:
PKG
cGK
RGS2
cGMP
PCA
luciferase
protein–protein interaction
Online Access:http://www.mdpi.com/1422-0067/19/4/1180

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