A Ubiquitously Expressed UDP-Glucosyltransferase, <i>UGT74J1</i>, Controls Basal Salicylic Acid Levels in Rice

• Main Authors: Daisuke Tezuka, Hideyuki Matsuura, Wataru Saburi, Haruhide Mori, Ryozo Imai
• Format: Article
• Language: English
• Published: MDPI AG 2021-09-01
• Series: Plants
• Subjects: salicylic acid; glycosylation; UDP-glucosyltransferase; disease resistance; <i>Pyricularia oryzae</i>
• Online Access: https://www.mdpi.com/2223-7747/10/9/1875

Salicylic acid (SA) is a phytohormone that regulates a variety of physiological and developmental processes, including disease resistance. SA is a key signaling component in the immune response of many plant species. However, the mechanism underlying SA-mediated immunity is obscure in rice (<i>Oryza sativa</i>). Prior analysis revealed a correlation between basal SA level and blast resistance in a range of rice varieties. This suggested that resistance might be improved by increasing basal SA level. Here, we identified a novel UDP-glucosyltransferase gene, <i>UGT74J1</i>, which is expressed ubiquitously throughout plant development. Mutants of <i>UGT74J1</i> generated by genome editing accumulated high levels of SA under non-stressed conditions, indicating that <i>UGT74J1</i> is a key enzyme for SA homeostasis in rice. Microarray analysis revealed that the <i>ugt74j1</i> mutants constitutively overexpressed a set of pathogenesis-related (PR) genes. An inoculation assay demonstrated that these mutants had increased resistance against rice blast, but they also exhibited stunted growth phenotypes. To our knowledge, this is the first report of a rice mutant displaying SA overaccumulation.