Liquid biopsy uncovers distinct patterns of DNA methylation and copy number changes in NSCLC patients with different EGFR-TKI resistant mutations
Abstract Targeted therapy with tyrosine kinase inhibitors (TKI) provides survival benefits to a majority of patients with non-small cell lung cancer (NSCLC). However, resistance to TKI almost always develops after treatment. Although genetic and epigenetic alterations have each been shown to drive r...
Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2021-08-01
|
Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-021-95985-6 |
id |
doaj-74f63e07641a43ec9278ce5ec5a27f64 |
---|---|
record_format |
Article |
spelling |
doaj-74f63e07641a43ec9278ce5ec5a27f642021-08-15T11:27:16ZengNature Publishing GroupScientific Reports2045-23222021-08-0111111210.1038/s41598-021-95985-6Liquid biopsy uncovers distinct patterns of DNA methylation and copy number changes in NSCLC patients with different EGFR-TKI resistant mutationsHoai-Nghia Nguyen0Ngoc-Phuong Thi Cao1Thien-Chi Van Nguyen2Khang Nguyen Duy Le3Dat Thanh Nguyen4Quynh-Tho Thi Nguyen5Thai-Hoa Thi Nguyen6Chu Van Nguyen7Ha Thu Le8Mai-Lan Thi Nguyen9Trieu Vu Nguyen10Vu Uyen Tran11Bac An Luong12Linh Gia Hoang Le13Quoc Chuong Ho14Hong-Anh Thi Pham15Binh Thanh Vo16Luan Thanh Nguyen17Anh-Thu Huynh Dang18Sinh Duy Nguyen19Duc Minh Do20Thanh-Thuy Thi Do21Anh Vu Hoang22Kiet Truong Dinh23Minh-Duy Phan24Hoa Giang25Le Son Tran26University of Medicine and Pharmacy at Ho Chi Minh CityMedical Genetics InstituteMedical Genetics InstituteMedical Genetics InstituteMedical Genetics InstituteMedical Genetics InstituteVietnam National Cancer HospitalVietnam National Cancer HospitalHa Noi Oncology HospitalHa Noi Oncology HospitalThu Duc District HospitalMedical Genetics InstituteUniversity of Medicine and Pharmacy at Ho Chi Minh CityUniversity of Medicine and Pharmacy at Ho Chi Minh CityUniversity of Medicine and Pharmacy at Ho Chi Minh CityMedical Genetics InstituteMedical Genetics InstituteMedical Genetics InstituteUniversity of Medicine and Pharmacy at Ho Chi Minh CityFV HospitalUniversity of Medicine and Pharmacy at Ho Chi Minh CityMedical Genetics InstituteUniversity of Medicine and Pharmacy at Ho Chi Minh CityMedical Genetics InstituteMedical Genetics InstituteMedical Genetics InstituteMedical Genetics InstituteAbstract Targeted therapy with tyrosine kinase inhibitors (TKI) provides survival benefits to a majority of patients with non-small cell lung cancer (NSCLC). However, resistance to TKI almost always develops after treatment. Although genetic and epigenetic alterations have each been shown to drive resistance to TKI in cell line models, clinical evidence for their contribution in the acquisition of resistance remains limited. Here, we employed liquid biopsy for simultaneous analysis of genetic and epigenetic changes in 122 Vietnamese NSCLC patients undergoing TKI therapy and displaying acquired resistance. We detected multiple profiles of resistance mutations in 51 patients (41.8%). Of those, genetic alterations in EGFR, particularly EGFR amplification (n = 6), showed pronounced genome instability and genome-wide hypomethylation. Interestingly, the level of hypomethylation was associated with the duration of response to TKI treatment. We also detected hypermethylation in regulatory regions of Homeobox genes which are known to be involved in tumor differentiation. In contrast, such changes were not observed in cases with MET (n = 4) and HER2 (n = 4) amplification. Thus, our study showed that liquid biopsy could provide important insights into the heterogeneity of TKI resistance mechanisms in NSCLC patients, providing essential information for prediction of resistance and selection of subsequent treatment.https://doi.org/10.1038/s41598-021-95985-6 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hoai-Nghia Nguyen Ngoc-Phuong Thi Cao Thien-Chi Van Nguyen Khang Nguyen Duy Le Dat Thanh Nguyen Quynh-Tho Thi Nguyen Thai-Hoa Thi Nguyen Chu Van Nguyen Ha Thu Le Mai-Lan Thi Nguyen Trieu Vu Nguyen Vu Uyen Tran Bac An Luong Linh Gia Hoang Le Quoc Chuong Ho Hong-Anh Thi Pham Binh Thanh Vo Luan Thanh Nguyen Anh-Thu Huynh Dang Sinh Duy Nguyen Duc Minh Do Thanh-Thuy Thi Do Anh Vu Hoang Kiet Truong Dinh Minh-Duy Phan Hoa Giang Le Son Tran |
spellingShingle |
Hoai-Nghia Nguyen Ngoc-Phuong Thi Cao Thien-Chi Van Nguyen Khang Nguyen Duy Le Dat Thanh Nguyen Quynh-Tho Thi Nguyen Thai-Hoa Thi Nguyen Chu Van Nguyen Ha Thu Le Mai-Lan Thi Nguyen Trieu Vu Nguyen Vu Uyen Tran Bac An Luong Linh Gia Hoang Le Quoc Chuong Ho Hong-Anh Thi Pham Binh Thanh Vo Luan Thanh Nguyen Anh-Thu Huynh Dang Sinh Duy Nguyen Duc Minh Do Thanh-Thuy Thi Do Anh Vu Hoang Kiet Truong Dinh Minh-Duy Phan Hoa Giang Le Son Tran Liquid biopsy uncovers distinct patterns of DNA methylation and copy number changes in NSCLC patients with different EGFR-TKI resistant mutations Scientific Reports |
author_facet |
Hoai-Nghia Nguyen Ngoc-Phuong Thi Cao Thien-Chi Van Nguyen Khang Nguyen Duy Le Dat Thanh Nguyen Quynh-Tho Thi Nguyen Thai-Hoa Thi Nguyen Chu Van Nguyen Ha Thu Le Mai-Lan Thi Nguyen Trieu Vu Nguyen Vu Uyen Tran Bac An Luong Linh Gia Hoang Le Quoc Chuong Ho Hong-Anh Thi Pham Binh Thanh Vo Luan Thanh Nguyen Anh-Thu Huynh Dang Sinh Duy Nguyen Duc Minh Do Thanh-Thuy Thi Do Anh Vu Hoang Kiet Truong Dinh Minh-Duy Phan Hoa Giang Le Son Tran |
author_sort |
Hoai-Nghia Nguyen |
title |
Liquid biopsy uncovers distinct patterns of DNA methylation and copy number changes in NSCLC patients with different EGFR-TKI resistant mutations |
title_short |
Liquid biopsy uncovers distinct patterns of DNA methylation and copy number changes in NSCLC patients with different EGFR-TKI resistant mutations |
title_full |
Liquid biopsy uncovers distinct patterns of DNA methylation and copy number changes in NSCLC patients with different EGFR-TKI resistant mutations |
title_fullStr |
Liquid biopsy uncovers distinct patterns of DNA methylation and copy number changes in NSCLC patients with different EGFR-TKI resistant mutations |
title_full_unstemmed |
Liquid biopsy uncovers distinct patterns of DNA methylation and copy number changes in NSCLC patients with different EGFR-TKI resistant mutations |
title_sort |
liquid biopsy uncovers distinct patterns of dna methylation and copy number changes in nsclc patients with different egfr-tki resistant mutations |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-08-01 |
description |
Abstract Targeted therapy with tyrosine kinase inhibitors (TKI) provides survival benefits to a majority of patients with non-small cell lung cancer (NSCLC). However, resistance to TKI almost always develops after treatment. Although genetic and epigenetic alterations have each been shown to drive resistance to TKI in cell line models, clinical evidence for their contribution in the acquisition of resistance remains limited. Here, we employed liquid biopsy for simultaneous analysis of genetic and epigenetic changes in 122 Vietnamese NSCLC patients undergoing TKI therapy and displaying acquired resistance. We detected multiple profiles of resistance mutations in 51 patients (41.8%). Of those, genetic alterations in EGFR, particularly EGFR amplification (n = 6), showed pronounced genome instability and genome-wide hypomethylation. Interestingly, the level of hypomethylation was associated with the duration of response to TKI treatment. We also detected hypermethylation in regulatory regions of Homeobox genes which are known to be involved in tumor differentiation. In contrast, such changes were not observed in cases with MET (n = 4) and HER2 (n = 4) amplification. Thus, our study showed that liquid biopsy could provide important insights into the heterogeneity of TKI resistance mechanisms in NSCLC patients, providing essential information for prediction of resistance and selection of subsequent treatment. |
url |
https://doi.org/10.1038/s41598-021-95985-6 |
work_keys_str_mv |
AT hoainghianguyen liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT ngocphuongthicao liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT thienchivannguyen liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT khangnguyenduyle liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT datthanhnguyen liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT quynhthothinguyen liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT thaihoathinguyen liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT chuvannguyen liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT hathule liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT mailanthinguyen liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT trieuvunguyen liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT vuuyentran liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT bacanluong liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT linhgiahoangle liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT quocchuongho liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT honganhthipham liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT binhthanhvo liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT luanthanhnguyen liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT anhthuhuynhdang liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT sinhduynguyen liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT ducminhdo liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT thanhthuythido liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT anhvuhoang liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT kiettruongdinh liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT minhduyphan liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT hoagiang liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations AT lesontran liquidbiopsyuncoversdistinctpatternsofdnamethylationandcopynumberchangesinnsclcpatientswithdifferentegfrtkiresistantmutations |
_version_ |
1721206748426010624 |