Twisting of DNA Origami from Intercalators
Abstract DNA nanostructures represent the confluence of materials science, computer science, biology, and engineering. As functional assemblies, they are capable of performing mechanical and chemical work. In this study, we demonstrate global twisting of DNA nanorails made from two DNA origami six-h...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2017-08-01
|
Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-017-07796-3 |
id |
doaj-75033a4fb23149ec99bcb5574ed145ab |
---|---|
record_format |
Article |
spelling |
doaj-75033a4fb23149ec99bcb5574ed145ab2020-12-08T00:38:01ZengNature Publishing GroupScientific Reports2045-23222017-08-01711510.1038/s41598-017-07796-3Twisting of DNA Origami from IntercalatorsReza M. Zadegan0Elias G. Lindau1William P. Klein2Christopher Green3Elton Graugnard4Bernard Yurke5Wan Kuang6William L. Hughes7Micron School of Materials Science & Engineering, Boise State UniversityMicron School of Materials Science & Engineering, Boise State UniversityMicron School of Materials Science & Engineering, Boise State UniversityMicron School of Materials Science & Engineering, Boise State UniversityMicron School of Materials Science & Engineering, Boise State UniversityDepartment of Electrical & Computer Engineering, Boise State UniversityDepartment of Electrical & Computer Engineering, Boise State UniversityMicron School of Materials Science & Engineering, Boise State UniversityAbstract DNA nanostructures represent the confluence of materials science, computer science, biology, and engineering. As functional assemblies, they are capable of performing mechanical and chemical work. In this study, we demonstrate global twisting of DNA nanorails made from two DNA origami six-helix bundles. Twisting was controlled using ethidium bromide or SYBR Green I as model intercalators. Our findings demonstrate that DNA nanorails: (i) twist when subjected to intercalators and the amount of twisting is concentration dependent, and (ii) twisting saturates at elevated concentrations. This study provides insight into how complex DNA structures undergo conformational changes when exposed to intercalators and may be of relevance when exploring how intercalating drugs interact with condensed biological structures such as chromatin and chromosomes, as well as chromatin analogous gene expression devices.https://doi.org/10.1038/s41598-017-07796-3 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Reza M. Zadegan Elias G. Lindau William P. Klein Christopher Green Elton Graugnard Bernard Yurke Wan Kuang William L. Hughes |
spellingShingle |
Reza M. Zadegan Elias G. Lindau William P. Klein Christopher Green Elton Graugnard Bernard Yurke Wan Kuang William L. Hughes Twisting of DNA Origami from Intercalators Scientific Reports |
author_facet |
Reza M. Zadegan Elias G. Lindau William P. Klein Christopher Green Elton Graugnard Bernard Yurke Wan Kuang William L. Hughes |
author_sort |
Reza M. Zadegan |
title |
Twisting of DNA Origami from Intercalators |
title_short |
Twisting of DNA Origami from Intercalators |
title_full |
Twisting of DNA Origami from Intercalators |
title_fullStr |
Twisting of DNA Origami from Intercalators |
title_full_unstemmed |
Twisting of DNA Origami from Intercalators |
title_sort |
twisting of dna origami from intercalators |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-08-01 |
description |
Abstract DNA nanostructures represent the confluence of materials science, computer science, biology, and engineering. As functional assemblies, they are capable of performing mechanical and chemical work. In this study, we demonstrate global twisting of DNA nanorails made from two DNA origami six-helix bundles. Twisting was controlled using ethidium bromide or SYBR Green I as model intercalators. Our findings demonstrate that DNA nanorails: (i) twist when subjected to intercalators and the amount of twisting is concentration dependent, and (ii) twisting saturates at elevated concentrations. This study provides insight into how complex DNA structures undergo conformational changes when exposed to intercalators and may be of relevance when exploring how intercalating drugs interact with condensed biological structures such as chromatin and chromosomes, as well as chromatin analogous gene expression devices. |
url |
https://doi.org/10.1038/s41598-017-07796-3 |
work_keys_str_mv |
AT rezamzadegan twistingofdnaorigamifromintercalators AT eliasglindau twistingofdnaorigamifromintercalators AT williampklein twistingofdnaorigamifromintercalators AT christophergreen twistingofdnaorigamifromintercalators AT eltongraugnard twistingofdnaorigamifromintercalators AT bernardyurke twistingofdnaorigamifromintercalators AT wankuang twistingofdnaorigamifromintercalators AT williamlhughes twistingofdnaorigamifromintercalators |
_version_ |
1724396091460288512 |