PEMILIHAN PRIMER RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) PADA PCR (POLYMERASE CHAIN REACTION) TANAMAN KAMBOJA (Plumeria sp.)

• Main Authors: Vanesa Martida, Made Pharmawati
• Format: Article
• Language: English
• Published: Universitas Udayana 2016-04-01
• Series: Simbiosis: Journal of Biological Sciences
• Subjects: DNA, Plumeria sp., RAPD primer
• Online Access: https://ojs.unud.ac.id/index.php/simbiosis/article/view/21226

There are many variation of Plumeria sp. that grown in Bali. The genetic identity of Plumeria sp. need to be analysed using molecular study for plant breeding purpose. DNA extraction and primer selection are basic steps for molecular study especially in identification and analysis of genetic diversity. The aim of this research was to determine RAPD primers suitable for molecular analysis of Plumeria sp. This research used CTAB method with modification for DNA extraction. The samples were young leaves of Plumeria sp. dried using silica gel. The primers used were produced by University of British Columbia and Operon Primer Technology. The results showed that DNA concentration of Plumeria sp from dried leaves was between 33-267 ng/?l. Out of seven primers tested, three primers UBC-127, UBC-250, and OPH-06 produced clear and scorable amplification products for further analyses. Keywords: DNA, Plumeria sp., RAPD primer