Delineating effect of corn microRNAs and matrix, ingested as whole food, on gut microbiota in a rodent model
Abstract Dietary microRNAs (miRNAs) are thought to regulate a wide range of biological processes, including the gut microbiota. However, it is difficult to separate specific effect(s) of miRNA from that of the food matrix. This study aims to elucidate the specific effect(s) of dietary corn miRNAs, i...
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Online Access: | https://doi.org/10.1002/fsn3.1672 |
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doaj-75289a4c930f4a6bb4a6037eac521fde2020-11-25T03:41:17ZengWileyFood Science & Nutrition2048-71772020-08-01884066407710.1002/fsn3.1672Delineating effect of corn microRNAs and matrix, ingested as whole food, on gut microbiota in a rodent modelHaiqiu Huang0Quynhchi Pham1Cindy D. Davis2Liangli Yu3Thomas T.Y. Wang4Diet, Genomics and Immunology Laboratory Beltsville Human Nutrition Research CenterUSDA‐ARS Beltsville Maryland USADiet, Genomics and Immunology Laboratory Beltsville Human Nutrition Research CenterUSDA‐ARS Beltsville Maryland USAOffice of Dietary Supplements NIH Bethesda Maryland USADepartment of Nutrition and Food Science University of Maryland College Park Maryland USADiet, Genomics and Immunology Laboratory Beltsville Human Nutrition Research CenterUSDA‐ARS Beltsville Maryland USAAbstract Dietary microRNAs (miRNAs) are thought to regulate a wide range of biological processes, including the gut microbiota. However, it is difficult to separate specific effect(s) of miRNA from that of the food matrix. This study aims to elucidate the specific effect(s) of dietary corn miRNAs, ingested as a whole food, on the gut microbiota. We developed an autoclave procedure to remove 98% of miRNA from corn. A mouse feeding study was conducted comparing autoclaved corn to nonautoclaved corn and purified corn miRNA. Compared to nonspecific nucleotides and corn devoid of miRNAs, feeding purified corn miRNAs or corn to C57BL/6 mice via gavage or diet supplementation for two weeks lead to a decrease in total bacteria in the cecum. The effect appeared to be due to changes in Firmicutes. Additionally, corn matrix minus miRNA and processing also affected gut bacteria. In silico analysis identified corn miRNAs that aligned to Firmicutes genome sequences lending further support to the interaction between corn miRNAs and this bacterium. These data support interactions between plant food miRNA, as well as matrix, and the gut microbiota exist but complex. However, it provides additional support for mechanism by which bioactive dietary components interact with the gut microbiota.https://doi.org/10.1002/fsn3.1672corndietary miRNAsFirmicutesgut microbiotaplant |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Haiqiu Huang Quynhchi Pham Cindy D. Davis Liangli Yu Thomas T.Y. Wang |
spellingShingle |
Haiqiu Huang Quynhchi Pham Cindy D. Davis Liangli Yu Thomas T.Y. Wang Delineating effect of corn microRNAs and matrix, ingested as whole food, on gut microbiota in a rodent model Food Science & Nutrition corn dietary miRNAs Firmicutes gut microbiota plant |
author_facet |
Haiqiu Huang Quynhchi Pham Cindy D. Davis Liangli Yu Thomas T.Y. Wang |
author_sort |
Haiqiu Huang |
title |
Delineating effect of corn microRNAs and matrix, ingested as whole food, on gut microbiota in a rodent model |
title_short |
Delineating effect of corn microRNAs and matrix, ingested as whole food, on gut microbiota in a rodent model |
title_full |
Delineating effect of corn microRNAs and matrix, ingested as whole food, on gut microbiota in a rodent model |
title_fullStr |
Delineating effect of corn microRNAs and matrix, ingested as whole food, on gut microbiota in a rodent model |
title_full_unstemmed |
Delineating effect of corn microRNAs and matrix, ingested as whole food, on gut microbiota in a rodent model |
title_sort |
delineating effect of corn micrornas and matrix, ingested as whole food, on gut microbiota in a rodent model |
publisher |
Wiley |
series |
Food Science & Nutrition |
issn |
2048-7177 |
publishDate |
2020-08-01 |
description |
Abstract Dietary microRNAs (miRNAs) are thought to regulate a wide range of biological processes, including the gut microbiota. However, it is difficult to separate specific effect(s) of miRNA from that of the food matrix. This study aims to elucidate the specific effect(s) of dietary corn miRNAs, ingested as a whole food, on the gut microbiota. We developed an autoclave procedure to remove 98% of miRNA from corn. A mouse feeding study was conducted comparing autoclaved corn to nonautoclaved corn and purified corn miRNA. Compared to nonspecific nucleotides and corn devoid of miRNAs, feeding purified corn miRNAs or corn to C57BL/6 mice via gavage or diet supplementation for two weeks lead to a decrease in total bacteria in the cecum. The effect appeared to be due to changes in Firmicutes. Additionally, corn matrix minus miRNA and processing also affected gut bacteria. In silico analysis identified corn miRNAs that aligned to Firmicutes genome sequences lending further support to the interaction between corn miRNAs and this bacterium. These data support interactions between plant food miRNA, as well as matrix, and the gut microbiota exist but complex. However, it provides additional support for mechanism by which bioactive dietary components interact with the gut microbiota. |
topic |
corn dietary miRNAs Firmicutes gut microbiota plant |
url |
https://doi.org/10.1002/fsn3.1672 |
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