Overexpression of a Novel <i>LcKNOX</i> Transcription Factor from <i>Liriodendron chinense</i> Induces Lobed Leaves in <i>Arabidopsis thaliana</i>
<i>Liriodendron chinense</i> is a common ornamental tree that has attractive leaves, which is a valuable trait for use in landscape architecture. In this work, we aimed to identify the potential genes that control and regulate the development of <i>L. chinense</i> leaf lobes....
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doaj-7543c877be8740428dd14b15a4ab4fa12020-11-25T01:13:07ZengMDPI AGForests1999-49072019-12-011113310.3390/f11010033f11010033Overexpression of a Novel <i>LcKNOX</i> Transcription Factor from <i>Liriodendron chinense</i> Induces Lobed Leaves in <i>Arabidopsis thaliana</i>Jikai Ma0Guoguo Mei1Huanhuan Liu2Huogen Li3Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaKey Laboratory of Forest Genetics & Biotechnology of Ministry of Education, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaKey Laboratory of Forest Genetics & Biotechnology of Ministry of Education, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaKey Laboratory of Forest Genetics & Biotechnology of Ministry of Education, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China<i>Liriodendron chinense</i> is a common ornamental tree that has attractive leaves, which is a valuable trait for use in landscape architecture. In this work, we aimed to identify the potential genes that control and regulate the development of <i>L. chinense</i> leaf lobes. Based on the transcriptome data for the leaf developmental stages we previously generated, two candidate genes were identified in this study. <i>KNOTTED-LIKE HOMEOBOX</i>(<i>KNOX</i>), encoding homeobox family proteins, play a large role in leaf lobe and leaf complexity regulation. Here, two full length <i>KNOX</i> genes from <i>L. chinense</i> were amplified and named <i>LcKNOX1</i> and <i>LcKNOX6</i> according to their sequence similarities with the respective <i>Arabidopsis thaliana KNOX</i> family genes. Overexpression vectors were constructed and subsequently transformed into wild type (WT) <i>A. thaliana</i>. Additionally, <i>LcKNOX6</i> was expressed in tobacco leaves to examine its subcellular localization, and the <i>35S::LcKNOX6</i> transgenic <i>A. thaliana</i> leaf cells were imaged with the use of SEM. The expression of several genes that participate in <i>KNOX</i> gene regulation were validated by quantitative real-time PCR. The results show that <i>LcKNOX1</i> produces almost the same phenotype as that found in WT <i>A. thaliana.</i> Notably, the <i>LcKNOX6-1</i> lines presented deep leaf lobes that were similar to <i>L. chinense</i> leaf lobes. Two <i>35S::LcKNOX6</i> lines induced an abnormal growth phenotype whose seeds were abortive. In short, these results indicate that the <i>LcKNOX6</i> gene might affect leaf development in <i>A. thaliana</i> and provide insights into the regulation of <i>L. chinense</i> leaf shaping.https://www.mdpi.com/1999-4907/11/1/33leaf shapeprimordia initiationaxes growthtranscription factorsphytohormone |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jikai Ma Guoguo Mei Huanhuan Liu Huogen Li |
spellingShingle |
Jikai Ma Guoguo Mei Huanhuan Liu Huogen Li Overexpression of a Novel <i>LcKNOX</i> Transcription Factor from <i>Liriodendron chinense</i> Induces Lobed Leaves in <i>Arabidopsis thaliana</i> Forests leaf shape primordia initiation axes growth transcription factors phytohormone |
author_facet |
Jikai Ma Guoguo Mei Huanhuan Liu Huogen Li |
author_sort |
Jikai Ma |
title |
Overexpression of a Novel <i>LcKNOX</i> Transcription Factor from <i>Liriodendron chinense</i> Induces Lobed Leaves in <i>Arabidopsis thaliana</i> |
title_short |
Overexpression of a Novel <i>LcKNOX</i> Transcription Factor from <i>Liriodendron chinense</i> Induces Lobed Leaves in <i>Arabidopsis thaliana</i> |
title_full |
Overexpression of a Novel <i>LcKNOX</i> Transcription Factor from <i>Liriodendron chinense</i> Induces Lobed Leaves in <i>Arabidopsis thaliana</i> |
title_fullStr |
Overexpression of a Novel <i>LcKNOX</i> Transcription Factor from <i>Liriodendron chinense</i> Induces Lobed Leaves in <i>Arabidopsis thaliana</i> |
title_full_unstemmed |
Overexpression of a Novel <i>LcKNOX</i> Transcription Factor from <i>Liriodendron chinense</i> Induces Lobed Leaves in <i>Arabidopsis thaliana</i> |
title_sort |
overexpression of a novel <i>lcknox</i> transcription factor from <i>liriodendron chinense</i> induces lobed leaves in <i>arabidopsis thaliana</i> |
publisher |
MDPI AG |
series |
Forests |
issn |
1999-4907 |
publishDate |
2019-12-01 |
description |
<i>Liriodendron chinense</i> is a common ornamental tree that has attractive leaves, which is a valuable trait for use in landscape architecture. In this work, we aimed to identify the potential genes that control and regulate the development of <i>L. chinense</i> leaf lobes. Based on the transcriptome data for the leaf developmental stages we previously generated, two candidate genes were identified in this study. <i>KNOTTED-LIKE HOMEOBOX</i>(<i>KNOX</i>), encoding homeobox family proteins, play a large role in leaf lobe and leaf complexity regulation. Here, two full length <i>KNOX</i> genes from <i>L. chinense</i> were amplified and named <i>LcKNOX1</i> and <i>LcKNOX6</i> according to their sequence similarities with the respective <i>Arabidopsis thaliana KNOX</i> family genes. Overexpression vectors were constructed and subsequently transformed into wild type (WT) <i>A. thaliana</i>. Additionally, <i>LcKNOX6</i> was expressed in tobacco leaves to examine its subcellular localization, and the <i>35S::LcKNOX6</i> transgenic <i>A. thaliana</i> leaf cells were imaged with the use of SEM. The expression of several genes that participate in <i>KNOX</i> gene regulation were validated by quantitative real-time PCR. The results show that <i>LcKNOX1</i> produces almost the same phenotype as that found in WT <i>A. thaliana.</i> Notably, the <i>LcKNOX6-1</i> lines presented deep leaf lobes that were similar to <i>L. chinense</i> leaf lobes. Two <i>35S::LcKNOX6</i> lines induced an abnormal growth phenotype whose seeds were abortive. In short, these results indicate that the <i>LcKNOX6</i> gene might affect leaf development in <i>A. thaliana</i> and provide insights into the regulation of <i>L. chinense</i> leaf shaping. |
topic |
leaf shape primordia initiation axes growth transcription factors phytohormone |
url |
https://www.mdpi.com/1999-4907/11/1/33 |
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