A novel model to culture cells from giant cell tumor of bone using three‐dimensional (3D) polycaprolactone scaffold
Abstract Two‐dimensional (2D) culture of cells from giant cell tumor of bone (GCTB) is affected by loss of the multinucleated giant cells in subsequent passages. Therefore, there is limited time to study GCTB with all its histological components in 2D culture. Here, we explored the possibility of cu...
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doaj-754585d0085a453d8884959a6a36b0982021-09-22T09:43:43ZengWiley-VCHEngineering in Life Sciences1618-02401618-28632021-09-01218-953954310.1002/elsc.202100020A novel model to culture cells from giant cell tumor of bone using three‐dimensional (3D) polycaprolactone scaffoldEréndira Estrada‐Villaseñor0Margarita Valdés‐Flores1Abelardo Meneses‐García2Phaedra Silva‐Bermudez3Raul Pichardo‐Bahena4Pedro Ostoa‐Saloma5Gabriela Mercado‐Celis6Ernesto D. Delgado‐Cedillo7Anell Olivos‐Meza8Carlos Landa‐Solís9Pathology Service National Institute of Rehabilitation Luis Guillermo Ibarra Ibarra Mexico City MexicoGenetics Laboratory National Institute of Rehabilitation Luis Guillermo Ibarra Ibarra Mexico City MexicoPathology Service National Cancer Institute Mexico City MexicoTissue Engineering and Cell Therapy Unit National Institute of Rehabilitation Luis Guillermo Ibarra Ibarra Mexico City MexicoPathology Service National Institute of Rehabilitation Luis Guillermo Ibarra Ibarra Mexico City MexicoDepartment of Immunology Institute of Biomedical Research National Autonomous University of Mexico Mexico City MexicoLaboratory of Clinical Genomics Division of Graduate Studies and Research Faculty of Odontology National Autonomous University of Mexico Mexico City MexicoBone Tumors Service National Institute of Rehabilitation Luis Guillermo Ibarra Ibarra Mexico City MexicoSports Orthopedics and Arthroscopy National Institute of Rehabilitation Luis Guillermo Ibarra Ibarra Mexico City MexicoTissue Engineering and Cell Therapy Unit National Institute of Rehabilitation Luis Guillermo Ibarra Ibarra Mexico City MexicoAbstract Two‐dimensional (2D) culture of cells from giant cell tumor of bone (GCTB) is affected by loss of the multinucleated giant cells in subsequent passages. Therefore, there is limited time to study GCTB with all its histological components in 2D culture. Here, we explored the possibility of culturing GCTB cells on a polycaprolactone (PCL)‐printed scaffold. We also evaluated the viability of the cultured cells and their adherence to the PCL scaffold at day 14 days using immunofluorescence analysis with calcein, vinculin, and phalloidin. Using the histological technique with hematoxylin and eosin staining, we observed all the histological components of GCTB in this 3D model. Immunohistochemical assays with cathepsin K, p63, and receptor activator of nuclear factor (NF)‐κB ligand (RANKL) yielded positive results in this construct, which allowed us to confirm that the seeded cells maintained the expression of GCTB markers. Based on these findings, we concluded that the PCL scaffold is an efficient model to culture GCTB cells, and the cell viability and adherence to the scaffold can be preserved for up to 14 days. Moreover, this model can also be used in subsequent studies to assess in vitro cell–cell interactions and antineoplastic efficacy of certain agents to establish a treatment against GCTB.https://doi.org/10.1002/elsc.2021000203D PCL scaffold3D tumor modelsgiant cell tumor of bone |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eréndira Estrada‐Villaseñor Margarita Valdés‐Flores Abelardo Meneses‐García Phaedra Silva‐Bermudez Raul Pichardo‐Bahena Pedro Ostoa‐Saloma Gabriela Mercado‐Celis Ernesto D. Delgado‐Cedillo Anell Olivos‐Meza Carlos Landa‐Solís |
spellingShingle |
Eréndira Estrada‐Villaseñor Margarita Valdés‐Flores Abelardo Meneses‐García Phaedra Silva‐Bermudez Raul Pichardo‐Bahena Pedro Ostoa‐Saloma Gabriela Mercado‐Celis Ernesto D. Delgado‐Cedillo Anell Olivos‐Meza Carlos Landa‐Solís A novel model to culture cells from giant cell tumor of bone using three‐dimensional (3D) polycaprolactone scaffold Engineering in Life Sciences 3D PCL scaffold 3D tumor models giant cell tumor of bone |
author_facet |
Eréndira Estrada‐Villaseñor Margarita Valdés‐Flores Abelardo Meneses‐García Phaedra Silva‐Bermudez Raul Pichardo‐Bahena Pedro Ostoa‐Saloma Gabriela Mercado‐Celis Ernesto D. Delgado‐Cedillo Anell Olivos‐Meza Carlos Landa‐Solís |
author_sort |
Eréndira Estrada‐Villaseñor |
title |
A novel model to culture cells from giant cell tumor of bone using three‐dimensional (3D) polycaprolactone scaffold |
title_short |
A novel model to culture cells from giant cell tumor of bone using three‐dimensional (3D) polycaprolactone scaffold |
title_full |
A novel model to culture cells from giant cell tumor of bone using three‐dimensional (3D) polycaprolactone scaffold |
title_fullStr |
A novel model to culture cells from giant cell tumor of bone using three‐dimensional (3D) polycaprolactone scaffold |
title_full_unstemmed |
A novel model to culture cells from giant cell tumor of bone using three‐dimensional (3D) polycaprolactone scaffold |
title_sort |
novel model to culture cells from giant cell tumor of bone using three‐dimensional (3d) polycaprolactone scaffold |
publisher |
Wiley-VCH |
series |
Engineering in Life Sciences |
issn |
1618-0240 1618-2863 |
publishDate |
2021-09-01 |
description |
Abstract Two‐dimensional (2D) culture of cells from giant cell tumor of bone (GCTB) is affected by loss of the multinucleated giant cells in subsequent passages. Therefore, there is limited time to study GCTB with all its histological components in 2D culture. Here, we explored the possibility of culturing GCTB cells on a polycaprolactone (PCL)‐printed scaffold. We also evaluated the viability of the cultured cells and their adherence to the PCL scaffold at day 14 days using immunofluorescence analysis with calcein, vinculin, and phalloidin. Using the histological technique with hematoxylin and eosin staining, we observed all the histological components of GCTB in this 3D model. Immunohistochemical assays with cathepsin K, p63, and receptor activator of nuclear factor (NF)‐κB ligand (RANKL) yielded positive results in this construct, which allowed us to confirm that the seeded cells maintained the expression of GCTB markers. Based on these findings, we concluded that the PCL scaffold is an efficient model to culture GCTB cells, and the cell viability and adherence to the scaffold can be preserved for up to 14 days. Moreover, this model can also be used in subsequent studies to assess in vitro cell–cell interactions and antineoplastic efficacy of certain agents to establish a treatment against GCTB. |
topic |
3D PCL scaffold 3D tumor models giant cell tumor of bone |
url |
https://doi.org/10.1002/elsc.202100020 |
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