Anastomotic stoma coated with chitosan film as a betamethasone dipropionate carrier for peripheral nerve regeneration
Scar hyperplasia at the suture site is an important reason for hindering the repair effect of peripheral nerve injury anastomosis. To address this issue, two repair methods are often used. Biological agents are used to block nerve sutures and the surrounding tissue to achieve physical anti-adhesion...
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Wolters Kluwer Medknow Publications
2018-01-01
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doaj-75476ed20b3e475fb389ffcff882bfae2020-11-25T03:40:38ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742018-01-0113230931610.4103/1673-5374.226401Anastomotic stoma coated with chitosan film as a betamethasone dipropionate carrier for peripheral nerve regenerationPing YaoPeng LiJun-jian JiangHong-ye LiScar hyperplasia at the suture site is an important reason for hindering the repair effect of peripheral nerve injury anastomosis. To address this issue, two repair methods are often used. Biological agents are used to block nerve sutures and the surrounding tissue to achieve physical anti-adhesion effects. Another agent is glucocorticosteroid, which can prevent scar growth by inhibiting inflammation. However, the overall effect of promoting regeneration of the injured nerve is not satisfactory. In this regard, we envision that these two methods can be combined and lead to shared understanding for achieving improved nerve repair. In this study, the right tibial nerve was transected 1 cm above the knee to establish a rat tibial nerve injury model. The incision was directly sutured after nerve transection. The anastomotic stoma was coated with 0.5 × 0.5 cm2 chitosan sheets with betamethasone dipropionate. At 12 weeks after injury, compared with the control and poly (D, L-lactic acid) groups, chitosan-betamethasone dipropionate film slowly degraded with the shape of the membrane still intact. Further, scar hyperplasia and the degree of adhesion at anastomotic stoma were obviously reduced, while the regenerated nerve fiber structure was complete and arranged in a good order in model rats. Electrophysiological study showed enhanced compound muscle action potential. Our results confirm that chitosan-betamethasone dipropionate film can effectively prevent local scar hyperplasia after tibial nerve repair and promote nerve regeneration.http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=2;spage=309;epage=316;aulast=Yaonerve regeneration; chitosan; betamethasone dipropionate; scar; nerve injury; repair; function restoration; film; drug release; carrier; neural regeneration |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ping Yao Peng Li Jun-jian Jiang Hong-ye Li |
spellingShingle |
Ping Yao Peng Li Jun-jian Jiang Hong-ye Li Anastomotic stoma coated with chitosan film as a betamethasone dipropionate carrier for peripheral nerve regeneration Neural Regeneration Research nerve regeneration; chitosan; betamethasone dipropionate; scar; nerve injury; repair; function restoration; film; drug release; carrier; neural regeneration |
author_facet |
Ping Yao Peng Li Jun-jian Jiang Hong-ye Li |
author_sort |
Ping Yao |
title |
Anastomotic stoma coated with chitosan film as a betamethasone dipropionate carrier for peripheral nerve regeneration |
title_short |
Anastomotic stoma coated with chitosan film as a betamethasone dipropionate carrier for peripheral nerve regeneration |
title_full |
Anastomotic stoma coated with chitosan film as a betamethasone dipropionate carrier for peripheral nerve regeneration |
title_fullStr |
Anastomotic stoma coated with chitosan film as a betamethasone dipropionate carrier for peripheral nerve regeneration |
title_full_unstemmed |
Anastomotic stoma coated with chitosan film as a betamethasone dipropionate carrier for peripheral nerve regeneration |
title_sort |
anastomotic stoma coated with chitosan film as a betamethasone dipropionate carrier for peripheral nerve regeneration |
publisher |
Wolters Kluwer Medknow Publications |
series |
Neural Regeneration Research |
issn |
1673-5374 |
publishDate |
2018-01-01 |
description |
Scar hyperplasia at the suture site is an important reason for hindering the repair effect of peripheral nerve injury anastomosis. To address this issue, two repair methods are often used. Biological agents are used to block nerve sutures and the surrounding tissue to achieve physical anti-adhesion effects. Another agent is glucocorticosteroid, which can prevent scar growth by inhibiting inflammation. However, the overall effect of promoting regeneration of the injured nerve is not satisfactory. In this regard, we envision that these two methods can be combined and lead to shared understanding for achieving improved nerve repair. In this study, the right tibial nerve was transected 1 cm above the knee to establish a rat tibial nerve injury model. The incision was directly sutured after nerve transection. The anastomotic stoma was coated with 0.5 × 0.5 cm2 chitosan sheets with betamethasone dipropionate. At 12 weeks after injury, compared with the control and poly (D, L-lactic acid) groups, chitosan-betamethasone dipropionate film slowly degraded with the shape of the membrane still intact. Further, scar hyperplasia and the degree of adhesion at anastomotic stoma were obviously reduced, while the regenerated nerve fiber structure was complete and arranged in a good order in model rats. Electrophysiological study showed enhanced compound muscle action potential. Our results confirm that chitosan-betamethasone dipropionate film can effectively prevent local scar hyperplasia after tibial nerve repair and promote nerve regeneration. |
topic |
nerve regeneration; chitosan; betamethasone dipropionate; scar; nerve injury; repair; function restoration; film; drug release; carrier; neural regeneration |
url |
http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=2;spage=309;epage=316;aulast=Yao |
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