Correspondence of Neutralizing Humoral Immunity and CD4 T Cell Responses in Long Recovered Sudan Virus Survivors

Robust humoral and cellular immunity are critical for survival in humans during an ebolavirus infection. However, the interplay between these two arms of immunity is poorly understood. To address this, we examined residual immune responses in survivors of the Sudan virus (SUDV) outbreak in Gulu, Uga...

Full description

Bibliographic Details
Main Authors:	Ariel Sobarzo, Spencer W. Stonier, Andrew S. Herbert, David E. Ochayon, Ana I. Kuehne, Yael Eskira, Shlomit Fedida-Metula, Neta Tali, Eli C. Lewis, Moses Egesa, Stephen Cose, Julius Julian Lutwama, Victoria Yavelsky, John M. Dye, Leslie Lobel
Format:	Article
Language:	English
Published:	MDPI AG 2016-05-01
Series:	Viruses
Subjects:	Ebola survivors memory immunity neutralizing antibodies cellular immunity
Online Access:	http://www.mdpi.com/1999-4915/8/5/133

id	doaj-754b80049c24467ea5acfd7f853c41c1
record_format	Article
spelling	doaj-754b80049c24467ea5acfd7f853c41c12020-11-24T23:33:59ZengMDPI AGViruses1999-49152016-05-018513310.3390/v8050133v8050133Correspondence of Neutralizing Humoral Immunity and CD4 T Cell Responses in Long Recovered Sudan Virus SurvivorsAriel Sobarzo0Spencer W. Stonier1Andrew S. Herbert2David E. Ochayon3Ana I. Kuehne4Yael Eskira5Shlomit Fedida-Metula6Neta Tali7Eli C. Lewis8Moses Egesa9Stephen Cose10Julius Julian Lutwama11Victoria Yavelsky12John M. Dye13Leslie Lobel14Department of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, IsraelVirology Division, U.S. Army Medical Research Institute of Infectious Diseases, 1425 Porter St., Fort Detrick, Frederick, MD 21701, USAVirology Division, U.S. Army Medical Research Institute of Infectious Diseases, 1425 Porter St., Fort Detrick, Frederick, MD 21701, USADepartment of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, IsraelVirology Division, U.S. Army Medical Research Institute of Infectious Diseases, 1425 Porter St., Fort Detrick, Frederick, MD 21701, USADepartment of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, IsraelDepartment of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, IsraelDepartment of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, IsraelDepartment of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, IsraelMedical Research Council/Uganda Virus Research Institute, Uganda Research Unit on AIDS, Entebbe P.O. Box 49, UgandaMedical Research Council/Uganda Virus Research Institute, Uganda Research Unit on AIDS, Entebbe P.O. Box 49, UgandaDepartment of Arbovirology, Emerging and Re-Emerging Infection Uganda Virus Research Institute, Entebbe P.O. Box 49, UgandaDepartment of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, IsraelVirology Division, U.S. Army Medical Research Institute of Infectious Diseases, 1425 Porter St., Fort Detrick, Frederick, MD 21701, USADepartment of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, IsraelRobust humoral and cellular immunity are critical for survival in humans during an ebolavirus infection. However, the interplay between these two arms of immunity is poorly understood. To address this, we examined residual immune responses in survivors of the Sudan virus (SUDV) outbreak in Gulu, Uganda (2000–2001). Cytokine and chemokine expression levels in SUDV stimulated whole blood cultures were assessed by multiplex ELISA and flow cytometry. Antibody and corresponding neutralization titers were also determined. Flow cytometry and multiplex ELISA results demonstrated significantly higher levels of cytokine and chemokine responses in survivors with serological neutralizing activity. This correspondence was not detected in survivors with serum reactivity to SUDV but without neutralization activity. This previously undefined relationship between memory CD4 T cell responses and serological neutralizing capacity in SUDV survivors is key for understanding long lasting immunity in survivors of filovirus infections.http://www.mdpi.com/1999-4915/8/5/133Ebola survivorsmemory immunityneutralizing antibodiescellular immunity
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Ariel Sobarzo Spencer W. Stonier Andrew S. Herbert David E. Ochayon Ana I. Kuehne Yael Eskira Shlomit Fedida-Metula Neta Tali Eli C. Lewis Moses Egesa Stephen Cose Julius Julian Lutwama Victoria Yavelsky John M. Dye Leslie Lobel
spellingShingle	Ariel Sobarzo Spencer W. Stonier Andrew S. Herbert David E. Ochayon Ana I. Kuehne Yael Eskira Shlomit Fedida-Metula Neta Tali Eli C. Lewis Moses Egesa Stephen Cose Julius Julian Lutwama Victoria Yavelsky John M. Dye Leslie Lobel Correspondence of Neutralizing Humoral Immunity and CD4 T Cell Responses in Long Recovered Sudan Virus Survivors Viruses Ebola survivors memory immunity neutralizing antibodies cellular immunity
author_facet	Ariel Sobarzo Spencer W. Stonier Andrew S. Herbert David E. Ochayon Ana I. Kuehne Yael Eskira Shlomit Fedida-Metula Neta Tali Eli C. Lewis Moses Egesa Stephen Cose Julius Julian Lutwama Victoria Yavelsky John M. Dye Leslie Lobel
author_sort	Ariel Sobarzo
title	Correspondence of Neutralizing Humoral Immunity and CD4 T Cell Responses in Long Recovered Sudan Virus Survivors
title_short	Correspondence of Neutralizing Humoral Immunity and CD4 T Cell Responses in Long Recovered Sudan Virus Survivors
title_full	Correspondence of Neutralizing Humoral Immunity and CD4 T Cell Responses in Long Recovered Sudan Virus Survivors
title_fullStr	Correspondence of Neutralizing Humoral Immunity and CD4 T Cell Responses in Long Recovered Sudan Virus Survivors
title_full_unstemmed	Correspondence of Neutralizing Humoral Immunity and CD4 T Cell Responses in Long Recovered Sudan Virus Survivors
title_sort	correspondence of neutralizing humoral immunity and cd4 t cell responses in long recovered sudan virus survivors
publisher	MDPI AG
series	Viruses
issn	1999-4915
publishDate	2016-05-01
description	Robust humoral and cellular immunity are critical for survival in humans during an ebolavirus infection. However, the interplay between these two arms of immunity is poorly understood. To address this, we examined residual immune responses in survivors of the Sudan virus (SUDV) outbreak in Gulu, Uganda (2000–2001). Cytokine and chemokine expression levels in SUDV stimulated whole blood cultures were assessed by multiplex ELISA and flow cytometry. Antibody and corresponding neutralization titers were also determined. Flow cytometry and multiplex ELISA results demonstrated significantly higher levels of cytokine and chemokine responses in survivors with serological neutralizing activity. This correspondence was not detected in survivors with serum reactivity to SUDV but without neutralization activity. This previously undefined relationship between memory CD4 T cell responses and serological neutralizing capacity in SUDV survivors is key for understanding long lasting immunity in survivors of filovirus infections.
topic	Ebola survivors memory immunity neutralizing antibodies cellular immunity
url	http://www.mdpi.com/1999-4915/8/5/133
work_keys_str_mv	AT arielsobarzo correspondenceofneutralizinghumoralimmunityandcd4tcellresponsesinlongrecoveredsudanvirussurvivors AT spencerwstonier correspondenceofneutralizinghumoralimmunityandcd4tcellresponsesinlongrecoveredsudanvirussurvivors AT andrewsherbert correspondenceofneutralizinghumoralimmunityandcd4tcellresponsesinlongrecoveredsudanvirussurvivors AT davideochayon correspondenceofneutralizinghumoralimmunityandcd4tcellresponsesinlongrecoveredsudanvirussurvivors AT anaikuehne correspondenceofneutralizinghumoralimmunityandcd4tcellresponsesinlongrecoveredsudanvirussurvivors AT yaeleskira correspondenceofneutralizinghumoralimmunityandcd4tcellresponsesinlongrecoveredsudanvirussurvivors AT shlomitfedidametula correspondenceofneutralizinghumoralimmunityandcd4tcellresponsesinlongrecoveredsudanvirussurvivors AT netatali correspondenceofneutralizinghumoralimmunityandcd4tcellresponsesinlongrecoveredsudanvirussurvivors AT eliclewis correspondenceofneutralizinghumoralimmunityandcd4tcellresponsesinlongrecoveredsudanvirussurvivors AT mosesegesa correspondenceofneutralizinghumoralimmunityandcd4tcellresponsesinlongrecoveredsudanvirussurvivors AT stephencose correspondenceofneutralizinghumoralimmunityandcd4tcellresponsesinlongrecoveredsudanvirussurvivors AT juliusjulianlutwama correspondenceofneutralizinghumoralimmunityandcd4tcellresponsesinlongrecoveredsudanvirussurvivors AT victoriayavelsky correspondenceofneutralizinghumoralimmunityandcd4tcellresponsesinlongrecoveredsudanvirussurvivors AT johnmdye correspondenceofneutralizinghumoralimmunityandcd4tcellresponsesinlongrecoveredsudanvirussurvivors AT leslielobel correspondenceofneutralizinghumoralimmunityandcd4tcellresponsesinlongrecoveredsudanvirussurvivors
_version_	1725530127187574784

Correspondence of Neutralizing Humoral Immunity and CD4 T Cell Responses in Long Recovered Sudan Virus Survivors

Similar Items