Biological Insights of Fluoroaryl-2,2′-Bichalcophene Compounds on Multi-Drug Resistant <i>Staphylococcus aureus</i>
Resistance of bacteria to multiple antibiotics is a significant health problem; hence, to continually respond to this challenge, different antibacterial agents must be constantly discovered. In this work, fluoroaryl-2,2′-bichalcophene derivatives were chemically synthesized and their biological acti...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-12-01
|
Series: | Molecules |
Subjects: | |
Online Access: | https://www.mdpi.com/1420-3049/26/1/139 |
id |
doaj-7557e19dbeac4681be933c20b2e3aa40 |
---|---|
record_format |
Article |
spelling |
doaj-7557e19dbeac4681be933c20b2e3aa402020-12-31T00:04:18ZengMDPI AGMolecules1420-30492021-12-012613913910.3390/molecules26010139Biological Insights of Fluoroaryl-2,2′-Bichalcophene Compounds on Multi-Drug Resistant <i>Staphylococcus aureus</i>Sally Elmogy0Mohamed A. Ismail1Rabeay Y. A. Hassan2Ahmed Noureldeen3Hadeer Darwish4Eman Fayad5Fahmy Elsaid6Ashraf Elsayed7Botany Department, Faculty of Science, Mansoura University, Elgomhouria St., Mansoura 35516, EgyptChemistry Department, Faculty of Science, Mansoura University, Elgomhouria St., Mansoura 35516, EgyptNanoscience Program, University of Science and Technology (UST), Zewail City of Science and Technology, 6th October City, Giza 12588, EgyptDepartment of Biology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biotechnology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biotechnology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaBiology Department, Faculty of Science, King Khalid University, P.O. Box 10347, Abha 61321, Saudi ArabiaBotany Department, Faculty of Science, Mansoura University, Elgomhouria St., Mansoura 35516, EgyptResistance of bacteria to multiple antibiotics is a significant health problem; hence, to continually respond to this challenge, different antibacterial agents must be constantly discovered. In this work, fluoroaryl-2,2′-bichalcophene derivatives were chemically synthesized and their biological activities were evaluated against <i>Staphylococcus aureus</i> (<i>S. aureus</i>). The impact of the investigated bichalcophene derivatives was studied on the ultrastructural level via scanning electron microscopy (SEM), molecular level via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method and on the biofilm inhibition via the electrochemical biosensors. Arylbichalcophenes’ antibacterial activity against <i>S. aureus</i> was affected by the presence and location of fluorine atoms. The fluorobithiophene derivative <b>MA-1156</b> displayed the best minimum inhibitory concentration (MIC) value of 16 µM among the tested fluoroarylbichalcophenes. Over a period of seven days, <i>S. aureus</i> did not develop any resistance against the tested fluoroarylbichalcophenes at higher concentrations. The impact of fluoroarylbichalcophenes was strong on <i>S. aureus</i> protein pattern showing high degrees of polymorphism. SEM micrographs of <i>S. aureus</i> cells treated with fluoroarylbichalcophenes displayed smaller cell-sizes, fewer numbers, arranged in a linear form and some of them were damaged when compared to the untreated cells. The bioelectrochemical measurements demonstrated the strong sensitivity of <i>S. aureus</i> cells to the tested fluoroarylbichalcophenes and an antibiofilm agent. Eventually, these fluoroarylbichalcophene compounds especially the <b>MA-1156</b> could be recommended as effective antibacterial agents.https://www.mdpi.com/1420-3049/26/1/139bichalcophene derivatives<i>S. aureus</i>antibacterial activityanti-biofilm agentsbiosensors |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sally Elmogy Mohamed A. Ismail Rabeay Y. A. Hassan Ahmed Noureldeen Hadeer Darwish Eman Fayad Fahmy Elsaid Ashraf Elsayed |
spellingShingle |
Sally Elmogy Mohamed A. Ismail Rabeay Y. A. Hassan Ahmed Noureldeen Hadeer Darwish Eman Fayad Fahmy Elsaid Ashraf Elsayed Biological Insights of Fluoroaryl-2,2′-Bichalcophene Compounds on Multi-Drug Resistant <i>Staphylococcus aureus</i> Molecules bichalcophene derivatives <i>S. aureus</i> antibacterial activity anti-biofilm agents biosensors |
author_facet |
Sally Elmogy Mohamed A. Ismail Rabeay Y. A. Hassan Ahmed Noureldeen Hadeer Darwish Eman Fayad Fahmy Elsaid Ashraf Elsayed |
author_sort |
Sally Elmogy |
title |
Biological Insights of Fluoroaryl-2,2′-Bichalcophene Compounds on Multi-Drug Resistant <i>Staphylococcus aureus</i> |
title_short |
Biological Insights of Fluoroaryl-2,2′-Bichalcophene Compounds on Multi-Drug Resistant <i>Staphylococcus aureus</i> |
title_full |
Biological Insights of Fluoroaryl-2,2′-Bichalcophene Compounds on Multi-Drug Resistant <i>Staphylococcus aureus</i> |
title_fullStr |
Biological Insights of Fluoroaryl-2,2′-Bichalcophene Compounds on Multi-Drug Resistant <i>Staphylococcus aureus</i> |
title_full_unstemmed |
Biological Insights of Fluoroaryl-2,2′-Bichalcophene Compounds on Multi-Drug Resistant <i>Staphylococcus aureus</i> |
title_sort |
biological insights of fluoroaryl-2,2′-bichalcophene compounds on multi-drug resistant <i>staphylococcus aureus</i> |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2021-12-01 |
description |
Resistance of bacteria to multiple antibiotics is a significant health problem; hence, to continually respond to this challenge, different antibacterial agents must be constantly discovered. In this work, fluoroaryl-2,2′-bichalcophene derivatives were chemically synthesized and their biological activities were evaluated against <i>Staphylococcus aureus</i> (<i>S. aureus</i>). The impact of the investigated bichalcophene derivatives was studied on the ultrastructural level via scanning electron microscopy (SEM), molecular level via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method and on the biofilm inhibition via the electrochemical biosensors. Arylbichalcophenes’ antibacterial activity against <i>S. aureus</i> was affected by the presence and location of fluorine atoms. The fluorobithiophene derivative <b>MA-1156</b> displayed the best minimum inhibitory concentration (MIC) value of 16 µM among the tested fluoroarylbichalcophenes. Over a period of seven days, <i>S. aureus</i> did not develop any resistance against the tested fluoroarylbichalcophenes at higher concentrations. The impact of fluoroarylbichalcophenes was strong on <i>S. aureus</i> protein pattern showing high degrees of polymorphism. SEM micrographs of <i>S. aureus</i> cells treated with fluoroarylbichalcophenes displayed smaller cell-sizes, fewer numbers, arranged in a linear form and some of them were damaged when compared to the untreated cells. The bioelectrochemical measurements demonstrated the strong sensitivity of <i>S. aureus</i> cells to the tested fluoroarylbichalcophenes and an antibiofilm agent. Eventually, these fluoroarylbichalcophene compounds especially the <b>MA-1156</b> could be recommended as effective antibacterial agents. |
topic |
bichalcophene derivatives <i>S. aureus</i> antibacterial activity anti-biofilm agents biosensors |
url |
https://www.mdpi.com/1420-3049/26/1/139 |
work_keys_str_mv |
AT sallyelmogy biologicalinsightsoffluoroaryl22bichalcophenecompoundsonmultidrugresistantistaphylococcusaureusi AT mohamedaismail biologicalinsightsoffluoroaryl22bichalcophenecompoundsonmultidrugresistantistaphylococcusaureusi AT rabeayyahassan biologicalinsightsoffluoroaryl22bichalcophenecompoundsonmultidrugresistantistaphylococcusaureusi AT ahmednoureldeen biologicalinsightsoffluoroaryl22bichalcophenecompoundsonmultidrugresistantistaphylococcusaureusi AT hadeerdarwish biologicalinsightsoffluoroaryl22bichalcophenecompoundsonmultidrugresistantistaphylococcusaureusi AT emanfayad biologicalinsightsoffluoroaryl22bichalcophenecompoundsonmultidrugresistantistaphylococcusaureusi AT fahmyelsaid biologicalinsightsoffluoroaryl22bichalcophenecompoundsonmultidrugresistantistaphylococcusaureusi AT ashrafelsayed biologicalinsightsoffluoroaryl22bichalcophenecompoundsonmultidrugresistantistaphylococcusaureusi |
_version_ |
1724365438138187776 |