A novel exopolysaccharide produced by Lactobacillus coryniformis NA-3 exhibits antioxidant and biofilm-inhibiting properties in vitro
Background: Exopolysaccharides (EPSs) secreted from lactic acid bacteria are carbohydrate polymers with reported biological activities. In this study, we extracted and characterized the composition as well as antioxidant and biofilm-inhibitory properties of EPS from Lactobacillus coryniformis NA-3 i...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Swedish Nutrition Foundation
2020-04-01
|
Series: | Food & Nutrition Research |
Subjects: | |
Online Access: | https://foodandnutritionresearch.net/index.php/fnr/article/view/3744/10279 |
id |
doaj-755993e9da6b4676ad25f884246e17c2 |
---|---|
record_format |
Article |
spelling |
doaj-755993e9da6b4676ad25f884246e17c22020-11-25T02:48:16ZengSwedish Nutrition FoundationFood & Nutrition Research1654-661X2020-04-0164011310.29219/fnr.v64.37443744A novel exopolysaccharide produced by Lactobacillus coryniformis NA-3 exhibits antioxidant and biofilm-inhibiting properties in vitroXiaoqing Xu0Qing Peng1Yuwei Zhang2Dandan Tian3Pengbo Zhang4Ying Huang5Lan Ma6Yu Qiao7Bo Shi8Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, PR ChinaFeed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, PR ChinaFeed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, PR ChinaFeed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, PR ChinaFeed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, PR ChinaFeed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, PR ChinaFeed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, PR ChinaFeed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, PR ChinaFeed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, PR ChinaBackground: Exopolysaccharides (EPSs) secreted from lactic acid bacteria are carbohydrate polymers with reported biological activities. In this study, we extracted and characterized the composition as well as antioxidant and biofilm-inhibitory properties of EPS from Lactobacillus coryniformis NA-3 isolated from northeast Chinese sauerkraut (Suan Cai). Methods: Lactobacillus coryniformis NA-3 was identified with 16S rDNA amplification and Neighbor Joining (NJ) phylogenetic analysis. EPS derived from Lactobacillus coryniformis NA-3 (EPS-NA3) was analyzed, including compositions by high-performance liquid chromatography (HPLC), functional groups by Fourier-transform infrared spectroscopy (FT-IR) and glycosidic bond configuration by Hydrogen-1 Nuclear Magnetic Resonance (1H NMR). Antioxidant activity of EPS was evaluated with hydroxyl and superoxide radical-scavenging. Anti-biofilm activities of EPS-NA3 were checked through inhibition and dispersion. Results: The monosaccharide composition of EPS included α-rhamnose, α-mannose, α-galactose, and α-glucose in a ratio of 2.6:1.0:5.0:3.3. The free radical-scavenging abilities of EPS-NA3 were 37.77% ± 1.56% and 78.87% ± 3.07% on hydroxyl and superoxide reactive oxygen species respectively. Moreover, EPS-NA3 attenuated the formation of Bacillus cereus and Salmonella typhimurium biofilms by inhibition ratios of approximately 80% and 40% respectively. Additionally, treatment with EPS-NA3 dispersed established biofilms of B. cereus and S. typhimurium by approximately 90% and 20% respectively. Conclusion: These results suggest that EPS-NA3 may be developed as antioxidant and anti-biofilm agents for industrial and clinical applications due to its capacity of scavenging free radicals, inhibition of bacterial biofilm formation, and dispersion of established biofilms.https://foodandnutritionresearch.net/index.php/fnr/article/view/3744/10279lactobacillus coryniformis na-3exopolysaccharideantioxidantanti-biofilminhibitiondispersion |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaoqing Xu Qing Peng Yuwei Zhang Dandan Tian Pengbo Zhang Ying Huang Lan Ma Yu Qiao Bo Shi |
spellingShingle |
Xiaoqing Xu Qing Peng Yuwei Zhang Dandan Tian Pengbo Zhang Ying Huang Lan Ma Yu Qiao Bo Shi A novel exopolysaccharide produced by Lactobacillus coryniformis NA-3 exhibits antioxidant and biofilm-inhibiting properties in vitro Food & Nutrition Research lactobacillus coryniformis na-3 exopolysaccharide antioxidant anti-biofilm inhibition dispersion |
author_facet |
Xiaoqing Xu Qing Peng Yuwei Zhang Dandan Tian Pengbo Zhang Ying Huang Lan Ma Yu Qiao Bo Shi |
author_sort |
Xiaoqing Xu |
title |
A novel exopolysaccharide produced by Lactobacillus coryniformis NA-3 exhibits antioxidant and biofilm-inhibiting properties in vitro |
title_short |
A novel exopolysaccharide produced by Lactobacillus coryniformis NA-3 exhibits antioxidant and biofilm-inhibiting properties in vitro |
title_full |
A novel exopolysaccharide produced by Lactobacillus coryniformis NA-3 exhibits antioxidant and biofilm-inhibiting properties in vitro |
title_fullStr |
A novel exopolysaccharide produced by Lactobacillus coryniformis NA-3 exhibits antioxidant and biofilm-inhibiting properties in vitro |
title_full_unstemmed |
A novel exopolysaccharide produced by Lactobacillus coryniformis NA-3 exhibits antioxidant and biofilm-inhibiting properties in vitro |
title_sort |
novel exopolysaccharide produced by lactobacillus coryniformis na-3 exhibits antioxidant and biofilm-inhibiting properties in vitro |
publisher |
Swedish Nutrition Foundation |
series |
Food & Nutrition Research |
issn |
1654-661X |
publishDate |
2020-04-01 |
description |
Background: Exopolysaccharides (EPSs) secreted from lactic acid bacteria are carbohydrate polymers with reported biological activities. In this study, we extracted and characterized the composition as well as antioxidant and biofilm-inhibitory properties of EPS from Lactobacillus coryniformis NA-3 isolated from northeast Chinese sauerkraut (Suan Cai). Methods: Lactobacillus coryniformis NA-3 was identified with 16S rDNA amplification and Neighbor Joining (NJ) phylogenetic analysis. EPS derived from Lactobacillus coryniformis NA-3 (EPS-NA3) was analyzed, including compositions by high-performance liquid chromatography (HPLC), functional groups by Fourier-transform infrared spectroscopy (FT-IR) and glycosidic bond configuration by Hydrogen-1 Nuclear Magnetic Resonance (1H NMR). Antioxidant activity of EPS was evaluated with hydroxyl and superoxide radical-scavenging. Anti-biofilm activities of EPS-NA3 were checked through inhibition and dispersion. Results: The monosaccharide composition of EPS included α-rhamnose, α-mannose, α-galactose, and α-glucose in a ratio of 2.6:1.0:5.0:3.3. The free radical-scavenging abilities of EPS-NA3 were 37.77% ± 1.56% and 78.87% ± 3.07% on hydroxyl and superoxide reactive oxygen species respectively. Moreover, EPS-NA3 attenuated the formation of Bacillus cereus and Salmonella typhimurium biofilms by inhibition ratios of approximately 80% and 40% respectively. Additionally, treatment with EPS-NA3 dispersed established biofilms of B. cereus and S. typhimurium by approximately 90% and 20% respectively. Conclusion: These results suggest that EPS-NA3 may be developed as antioxidant and anti-biofilm agents for industrial and clinical applications due to its capacity of scavenging free radicals, inhibition of bacterial biofilm formation, and dispersion of established biofilms. |
topic |
lactobacillus coryniformis na-3 exopolysaccharide antioxidant anti-biofilm inhibition dispersion |
url |
https://foodandnutritionresearch.net/index.php/fnr/article/view/3744/10279 |
work_keys_str_mv |
AT xiaoqingxu anovelexopolysaccharideproducedbylactobacilluscoryniformisna3exhibitsantioxidantandbiofilminhibitingpropertiesinvitro AT qingpeng anovelexopolysaccharideproducedbylactobacilluscoryniformisna3exhibitsantioxidantandbiofilminhibitingpropertiesinvitro AT yuweizhang anovelexopolysaccharideproducedbylactobacilluscoryniformisna3exhibitsantioxidantandbiofilminhibitingpropertiesinvitro AT dandantian anovelexopolysaccharideproducedbylactobacilluscoryniformisna3exhibitsantioxidantandbiofilminhibitingpropertiesinvitro AT pengbozhang anovelexopolysaccharideproducedbylactobacilluscoryniformisna3exhibitsantioxidantandbiofilminhibitingpropertiesinvitro AT yinghuang anovelexopolysaccharideproducedbylactobacilluscoryniformisna3exhibitsantioxidantandbiofilminhibitingpropertiesinvitro AT lanma anovelexopolysaccharideproducedbylactobacilluscoryniformisna3exhibitsantioxidantandbiofilminhibitingpropertiesinvitro AT yuqiao anovelexopolysaccharideproducedbylactobacilluscoryniformisna3exhibitsantioxidantandbiofilminhibitingpropertiesinvitro AT boshi anovelexopolysaccharideproducedbylactobacilluscoryniformisna3exhibitsantioxidantandbiofilminhibitingpropertiesinvitro AT xiaoqingxu novelexopolysaccharideproducedbylactobacilluscoryniformisna3exhibitsantioxidantandbiofilminhibitingpropertiesinvitro AT qingpeng novelexopolysaccharideproducedbylactobacilluscoryniformisna3exhibitsantioxidantandbiofilminhibitingpropertiesinvitro AT yuweizhang novelexopolysaccharideproducedbylactobacilluscoryniformisna3exhibitsantioxidantandbiofilminhibitingpropertiesinvitro AT dandantian novelexopolysaccharideproducedbylactobacilluscoryniformisna3exhibitsantioxidantandbiofilminhibitingpropertiesinvitro AT pengbozhang novelexopolysaccharideproducedbylactobacilluscoryniformisna3exhibitsantioxidantandbiofilminhibitingpropertiesinvitro AT yinghuang novelexopolysaccharideproducedbylactobacilluscoryniformisna3exhibitsantioxidantandbiofilminhibitingpropertiesinvitro AT lanma novelexopolysaccharideproducedbylactobacilluscoryniformisna3exhibitsantioxidantandbiofilminhibitingpropertiesinvitro AT yuqiao novelexopolysaccharideproducedbylactobacilluscoryniformisna3exhibitsantioxidantandbiofilminhibitingpropertiesinvitro AT boshi novelexopolysaccharideproducedbylactobacilluscoryniformisna3exhibitsantioxidantandbiofilminhibitingpropertiesinvitro |
_version_ |
1724748823363846144 |