Light-dependent N-end rule-mediated disruption of protein function in Saccharomyces cerevisiae and Drosophila melanogaster.
Here we describe the development and characterization of the photo-N-degron, a peptide tag that can be used in optogenetic studies of protein function in vivo. The photo-N-degron can be expressed as a genetic fusion to the amino termini of other proteins, where it undergoes a blue light-dependent co...
Main Authors: | Leslie M Stevens, Goheun Kim, Theodora Koromila, John W Steele, James McGehee, Angelike Stathopoulos, David S Stein |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2021-05-01
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Series: | PLoS Genetics |
Online Access: | https://doi.org/10.1371/journal.pgen.1009544 |
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