Meta-analysis: Association between promoter hypermethylation of Glutathione S-Transferase P1 (GSTP1) and breastcancer

• Main Authors: Trương Kim Phương, Phạm Hoàng Năng, Lao Đức Thuận, Lê Huyền Ái Thuý
• Format: Article
• Language: English
• Published: HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE 2018-10-01
• Series: Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ
• Subjects: gstp1; methyl hóa; phân tích tổng hợp; ung thư vú; breast cancer; meta analysis; methylation
• Online Access: https://journalofscience.ou.edu.vn/index.php/tech-vi/article/view/799

The association between promoter hypermethylation of Glutathione S-transferase P1 (GSTP1) and breast cancer has been investigated by much previous research worldwide. However, the results of the correlation between the epigenetic features of human genes with breast cancer varied from study to study. The purpose of this study, therefore, is to use a meta-analysis to identify how the GSTP1gene’s promoter hypermethylation and breast cancer correlate to each other. Accordingly, 19 case-control studies were conducted to evaluate the association between GSTP gene’ promoter methylation status and breast cancer, including 1910 cancer cases and 671 control cases. The findings showed that the status of gene promoter methylation increased the candidate’s breast cancer risk by calculating the OR value (OR = 10,497; 95%CI = 4.42 -24.94; P < 0.0001; Random-effect meta-analysis). In addition, the re-collection of 13 case-control studies, including 1247 cancer cases and 369 control case, also showed an increase in the OR value (OR = 13,642; 95%CI = 8.23 -22.60; P < 0,001, Fixed-effect meta-analysis model). The association value also increased. Then, the hypermethylation characteristic of the GSTP1 gene’s promoter was observed in both tissue and blood samples, and at this level, it should be detected by many methods such as Methylation Specific PCR, MS-MLPA, Methylight and QMSP compared to Bisulfite methylation-specific PCR. The hypermethylated GSTP1 promoter was higher in Europeans and Africans than in Asians. Moreover, the hypermethylated GSTP1gene’s promoter is also found in breast cancer patients with high histopathology, large tumor size, lymph node and HER2 and at the terminal stage of the disease. The hypermethylated GSTP1gene’s promoter also results in the reduced expression and activity of GSTP1 protein.