Associations between cytokine gene polymorphisms and rheumatoid arthritis in Turkish population
OBJECTIVE: Various cytokine polymorphisms have been associated with genetic risk factors predisposing to Rheumatoid Arthritis (RA) in different populations. To predict the clinical outcome as well as response to therapy in RA, studies aimed to describe genetic markers. The present study aims to sear...
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doaj-7592698b28204817b6b667034a6c9add2021-01-24T18:41:08ZengKARE Publishingİstanbul Kuzey Klinikleri2148-49022020-12-017656357110.14744/nci.2020.70845NCI-70845Associations between cytokine gene polymorphisms and rheumatoid arthritis in Turkish populationBurcu Yucel0Ceren Sumer1Ilhami Gok2Murat Karkucak3Emel Alemdaroglu4Fahri Ucar5Department of Medical Biology, Istanbul Medeniyet University Faculty of Medicine, Istanbul, TurkeyDepartment of Medical Biology, Karadeniz Technical University Faculty of Medicine, Trabzon, TurkeyDepartment of Bioengineering, Kafkas University Faculty of Engineering and Architecture, Kars, TurkeyDivision of Rheumatology, Department of Physical Medicine and Rehabilitation, Karadeniz Technical University, Farabi Hospital, Trabzon, TurkeyDepartment of Medical Biology, Karadeniz Technical University Faculty of Medicine, Trabzon, TurkeyDepartment of Medical Biology and Genetics, Akdeniz University Faculty of Medicine, Antalya, TurkeyOBJECTIVE: Various cytokine polymorphisms have been associated with genetic risk factors predisposing to Rheumatoid Arthritis (RA) in different populations. To predict the clinical outcome as well as response to therapy in RA, studies aimed to describe genetic markers. The present study aims to search for polymorphisms of 13 cytokine coding genes in the Eastern Black Sea Region of Turkey. METHODS: DNAs of 49 patients and 96 healthy bone marrow and kidney donors were isolated from peripheral blood samples. Genotyping was performed using the Heidelberg Cytokine Typing Tray kit. PCR products were visualized on an agarose gel, and results were analyzed using the interpretation scheme provided with the kit. Arlequin 3.5 software was used for statistical analysis. RESULTS: No positive association was found between allele frequencies and the disease. However, a negative association was found for the IL-A -889 C allele (p=0.02, OR=0.533, Wald's 95% CI=0.318–0.893). IL-12 -1188 CC (p=0.01, OR=3.667, Wald's 95% CI=1.246–10.786), IL-4 -1098 GT (p=0.02, OR=2.405, Wald's 95% CI=1.129–5.125) genotypes were found positively associated with the RA, while IL-4 -590 CT (p=0.02, OR=0.422, Wald's 95% CI=0.201–0.886) was found negatively associated with the disease. In addition, IL-6 GG haplotype was found positively associated with the RA (p=0.02, OR=1.880, Wald's 95% CI=1.086–3.254). CONCLUSION: Our findings suggest that some polymorphisms of the IL-1A, IL-2, IL-4, IL-6 and IL-12 could be responsible for the susceptibility or protective to RA in our study population. Multi-centered and large numbers of subjects containing studies that search for cytokine polymorphisms will gather more information regarding the susceptibility to RA of Turkish patients.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=nci&un=NCI-70845cytokine polymorphismpcr-ssp; rheumatoid arthritis. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Burcu Yucel Ceren Sumer Ilhami Gok Murat Karkucak Emel Alemdaroglu Fahri Ucar |
spellingShingle |
Burcu Yucel Ceren Sumer Ilhami Gok Murat Karkucak Emel Alemdaroglu Fahri Ucar Associations between cytokine gene polymorphisms and rheumatoid arthritis in Turkish population İstanbul Kuzey Klinikleri cytokine polymorphism pcr-ssp; rheumatoid arthritis. |
author_facet |
Burcu Yucel Ceren Sumer Ilhami Gok Murat Karkucak Emel Alemdaroglu Fahri Ucar |
author_sort |
Burcu Yucel |
title |
Associations between cytokine gene polymorphisms and rheumatoid arthritis in Turkish population |
title_short |
Associations between cytokine gene polymorphisms and rheumatoid arthritis in Turkish population |
title_full |
Associations between cytokine gene polymorphisms and rheumatoid arthritis in Turkish population |
title_fullStr |
Associations between cytokine gene polymorphisms and rheumatoid arthritis in Turkish population |
title_full_unstemmed |
Associations between cytokine gene polymorphisms and rheumatoid arthritis in Turkish population |
title_sort |
associations between cytokine gene polymorphisms and rheumatoid arthritis in turkish population |
publisher |
KARE Publishing |
series |
İstanbul Kuzey Klinikleri |
issn |
2148-4902 |
publishDate |
2020-12-01 |
description |
OBJECTIVE: Various cytokine polymorphisms have been associated with genetic risk factors predisposing to Rheumatoid Arthritis (RA) in different populations. To predict the clinical outcome as well as response to therapy in RA, studies aimed to describe genetic markers. The present study aims to search for polymorphisms of 13 cytokine coding genes in the Eastern Black Sea Region of Turkey.
METHODS: DNAs of 49 patients and 96 healthy bone marrow and kidney donors were isolated from peripheral blood samples. Genotyping was performed using the Heidelberg Cytokine Typing Tray kit. PCR products were visualized on an agarose gel, and results were analyzed using the interpretation scheme provided with the kit. Arlequin 3.5 software was used for statistical analysis.
RESULTS: No positive association was found between allele frequencies and the disease. However, a negative association was found for the IL-A -889 C allele (p=0.02, OR=0.533, Wald's 95% CI=0.318–0.893). IL-12 -1188 CC (p=0.01, OR=3.667, Wald's 95% CI=1.246–10.786), IL-4 -1098 GT (p=0.02, OR=2.405, Wald's 95% CI=1.129–5.125) genotypes were found positively associated with the RA, while IL-4 -590 CT (p=0.02, OR=0.422, Wald's 95% CI=0.201–0.886) was found negatively associated with the disease. In addition, IL-6 GG haplotype was found positively associated with the RA (p=0.02, OR=1.880, Wald's 95% CI=1.086–3.254).
CONCLUSION: Our findings suggest that some polymorphisms of the IL-1A, IL-2, IL-4, IL-6 and IL-12 could be responsible for the susceptibility or protective to RA in our study population. Multi-centered and large numbers of subjects containing studies that search for cytokine polymorphisms will gather more information regarding the susceptibility to RA of Turkish patients. |
topic |
cytokine polymorphism pcr-ssp; rheumatoid arthritis. |
url |
https://jag.journalagent.com/z4/download_fulltext.asp?pdir=nci&un=NCI-70845 |
work_keys_str_mv |
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