Polymorphic analysis of leptin promoter in obese/diabetic subjects in Kashmiri population
Background: The role of common variants in leptin promoter has already been established to play a major role in obesity and diabetes in humans. The study was accordingly focused on leptin promoter variants and their potential association with diabetes and obesity in ethnic population from Kashmir, I...
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2019-01-01
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doaj-75a3893d25064abdb36492f0e4e890d12020-11-25T01:49:53ZengWolters Kluwer Medknow PublicationsIndian Journal of Endocrinology and Metabolism2230-82102019-01-0123111111610.4103/ijem.IJEM_164_18Polymorphic analysis of leptin promoter in obese/diabetic subjects in Kashmiri populationRubiya DarShabhat RasoolAjaz Ahmad WazaGazalla AyoubMeenu QureshiAbdul Hamid ZargarIftikhar BashirTariq JanKhurshid Iqbal AndrabiBackground: The role of common variants in leptin promoter has already been established to play a major role in obesity and diabetes in humans. The study was accordingly focused on leptin promoter variants and their potential association with diabetes and obesity in ethnic population from Kashmir, India. Methods: Allele frequencies of 620 Kashmiri subjects with diabetes (200), obese subjects (200), and ethnically matched healthy controls (200) were tested for the Hardy–Weinberg disequilibrium. Among 200 obese subjects, a total of 50 persons were with diabetes. The genotype and allele frequencies were evaluated using the Chi-square or Fisher's exact tests. Results: Sequence analysis revealed two reported variations i.e., rs72563764C>T and rs7799039G>A in promoter region. Both variants show homozygous as well as heterozygous genotypes. These variations indicated significant difference with respect to allelic and genotypic frequencies in all groups i.e., persons with diabetes, obese, and obese persons with diabetes (P < 0.05). We also analyzed the association of these variations with biochemical characteristics and found significant association of rs72563764C>T with triglycerides (TG) in obese patients and fasting plasma glucose (FPG) and random blood sugar (RBS) in obese/persons with diabetes. Also rs7799039G>A showed association with postprandial plasma sugar (PPPS) in obese patients and FPG and resting plasma glucose (RPG) in obese persons with diabetes. Conclusions: Our results are suggestive of the association of leptin promoter gene variations i.e., rs72563764C>T and rs7799039G>A with both diabetes and obesity.http://www.ijem.in/article.asp?issn=2230-8210;year=2019;volume=23;issue=1;spage=111;epage=116;aulast=Dardiabetesleptinleptin receptorobesitysingle-nucleotide polymorphisms |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rubiya Dar Shabhat Rasool Ajaz Ahmad Waza Gazalla Ayoub Meenu Qureshi Abdul Hamid Zargar Iftikhar Bashir Tariq Jan Khurshid Iqbal Andrabi |
spellingShingle |
Rubiya Dar Shabhat Rasool Ajaz Ahmad Waza Gazalla Ayoub Meenu Qureshi Abdul Hamid Zargar Iftikhar Bashir Tariq Jan Khurshid Iqbal Andrabi Polymorphic analysis of leptin promoter in obese/diabetic subjects in Kashmiri population Indian Journal of Endocrinology and Metabolism diabetes leptin leptin receptor obesity single-nucleotide polymorphisms |
author_facet |
Rubiya Dar Shabhat Rasool Ajaz Ahmad Waza Gazalla Ayoub Meenu Qureshi Abdul Hamid Zargar Iftikhar Bashir Tariq Jan Khurshid Iqbal Andrabi |
author_sort |
Rubiya Dar |
title |
Polymorphic analysis of leptin promoter in obese/diabetic subjects in Kashmiri population |
title_short |
Polymorphic analysis of leptin promoter in obese/diabetic subjects in Kashmiri population |
title_full |
Polymorphic analysis of leptin promoter in obese/diabetic subjects in Kashmiri population |
title_fullStr |
Polymorphic analysis of leptin promoter in obese/diabetic subjects in Kashmiri population |
title_full_unstemmed |
Polymorphic analysis of leptin promoter in obese/diabetic subjects in Kashmiri population |
title_sort |
polymorphic analysis of leptin promoter in obese/diabetic subjects in kashmiri population |
publisher |
Wolters Kluwer Medknow Publications |
series |
Indian Journal of Endocrinology and Metabolism |
issn |
2230-8210 |
publishDate |
2019-01-01 |
description |
Background: The role of common variants in leptin promoter has already been established to play a major role in obesity and diabetes in humans. The study was accordingly focused on leptin promoter variants and their potential association with diabetes and obesity in ethnic population from Kashmir, India. Methods: Allele frequencies of 620 Kashmiri subjects with diabetes (200), obese subjects (200), and ethnically matched healthy controls (200) were tested for the Hardy–Weinberg disequilibrium. Among 200 obese subjects, a total of 50 persons were with diabetes. The genotype and allele frequencies were evaluated using the Chi-square or Fisher's exact tests. Results: Sequence analysis revealed two reported variations i.e., rs72563764C>T and rs7799039G>A in promoter region. Both variants show homozygous as well as heterozygous genotypes. These variations indicated significant difference with respect to allelic and genotypic frequencies in all groups i.e., persons with diabetes, obese, and obese persons with diabetes (P < 0.05). We also analyzed the association of these variations with biochemical characteristics and found significant association of rs72563764C>T with triglycerides (TG) in obese patients and fasting plasma glucose (FPG) and random blood sugar (RBS) in obese/persons with diabetes. Also rs7799039G>A showed association with postprandial plasma sugar (PPPS) in obese patients and FPG and resting plasma glucose (RPG) in obese persons with diabetes. Conclusions: Our results are suggestive of the association of leptin promoter gene variations i.e., rs72563764C>T and rs7799039G>A with both diabetes and obesity. |
topic |
diabetes leptin leptin receptor obesity single-nucleotide polymorphisms |
url |
http://www.ijem.in/article.asp?issn=2230-8210;year=2019;volume=23;issue=1;spage=111;epage=116;aulast=Dar |
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