Prognostic Impact of Melatonin Receptors MT1 and MT2 in Non-Small Cell Lung Cancer (NSCLC)
<i>Background</i>: Several studies have investigated the inhibitory effect of melatonin on lung cancer cells. There are no data available on the prognostic impact of melatonin receptors MT1 and MT2 in non-small cell lung cancer (NSCLC). <i>Materials and Methods</i>: Immunohis...
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doaj-75a48ec3203a48ea8d02791a5e58b8822020-11-24T20:44:10ZengMDPI AGCancers2072-66942019-07-01117100110.3390/cancers11071001cancers11071001Prognostic Impact of Melatonin Receptors MT1 and MT2 in Non-Small Cell Lung Cancer (NSCLC)Karolina Jablonska0Katarzyna Nowinska1Aleksandra Piotrowska2Aleksandra Partynska3Ewa Katnik4Konrad Pawelczyk5Alicja Kmiecik6Natalia Glatzel-Plucinska7Marzenna Podhorska-Okolow8Piotr Dziegiel9Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandDivision of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandDivision of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandDivision of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandDivision of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandDepartment of Thoracic Surgery, Wroclaw Medical University, 53-439 Wroclaw, PolandDivision of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandDivision of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandDivision of Ultrastructure Research, Wroclaw Medical University, 50-368 Wroclaw, PolandDivision of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland<i>Background</i>: Several studies have investigated the inhibitory effect of melatonin on lung cancer cells. There are no data available on the prognostic impact of melatonin receptors MT1 and MT2 in non-small cell lung cancer (NSCLC). <i>Materials and Methods</i>: Immunohistochemical studies of MT1 and MT2 were conducted on NSCLC (N = 786) and non-malignant lung tissue (NMLT) (N = 120) using tissue microarrays. Molecular studies were performed on frozen fragments of NSCLC (N = 62; real time PCR), NMLT (N = 24) and lung cancer cell lines NCI-H1703, A549 and IMR-90 (real time PCR, western blot). <i>Results</i>: The expression of both receptors was higher in NSCLC than in NMLT. Higher MT1 and MT2 expression levels (at protein and mRNA) were noted in squamous cell carcinomas (SCC) compared to adenocarcinomas (AC). MT1 immunoexpression decreased as both the tumour size and the cancer stage increased in the whole cohort, while MT2 decreased as the cancer stage increased, with lymph node involvement (in the whole study group) and increasing malignancy grade (in SCC). Higher expression of MT2 was associated with a favorable prognosis. MT2 was an independent prognostic factor for overall survival (OS) in all analyzed NSCLC and in smoking patients. <i>Conclusions</i>: Our observations may point to the potential prognostic significance of MT2 in NSCLC.https://www.mdpi.com/2072-6694/11/7/1001melatoninmelatonin receptorMT1MT2non-small cell lung carcinomaimmunohistochemistrycancer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Karolina Jablonska Katarzyna Nowinska Aleksandra Piotrowska Aleksandra Partynska Ewa Katnik Konrad Pawelczyk Alicja Kmiecik Natalia Glatzel-Plucinska Marzenna Podhorska-Okolow Piotr Dziegiel |
spellingShingle |
Karolina Jablonska Katarzyna Nowinska Aleksandra Piotrowska Aleksandra Partynska Ewa Katnik Konrad Pawelczyk Alicja Kmiecik Natalia Glatzel-Plucinska Marzenna Podhorska-Okolow Piotr Dziegiel Prognostic Impact of Melatonin Receptors MT1 and MT2 in Non-Small Cell Lung Cancer (NSCLC) Cancers melatonin melatonin receptor MT1 MT2 non-small cell lung carcinoma immunohistochemistry cancer |
author_facet |
Karolina Jablonska Katarzyna Nowinska Aleksandra Piotrowska Aleksandra Partynska Ewa Katnik Konrad Pawelczyk Alicja Kmiecik Natalia Glatzel-Plucinska Marzenna Podhorska-Okolow Piotr Dziegiel |
author_sort |
Karolina Jablonska |
title |
Prognostic Impact of Melatonin Receptors MT1 and MT2 in Non-Small Cell Lung Cancer (NSCLC) |
title_short |
Prognostic Impact of Melatonin Receptors MT1 and MT2 in Non-Small Cell Lung Cancer (NSCLC) |
title_full |
Prognostic Impact of Melatonin Receptors MT1 and MT2 in Non-Small Cell Lung Cancer (NSCLC) |
title_fullStr |
Prognostic Impact of Melatonin Receptors MT1 and MT2 in Non-Small Cell Lung Cancer (NSCLC) |
title_full_unstemmed |
Prognostic Impact of Melatonin Receptors MT1 and MT2 in Non-Small Cell Lung Cancer (NSCLC) |
title_sort |
prognostic impact of melatonin receptors mt1 and mt2 in non-small cell lung cancer (nsclc) |
publisher |
MDPI AG |
series |
Cancers |
issn |
2072-6694 |
publishDate |
2019-07-01 |
description |
<i>Background</i>: Several studies have investigated the inhibitory effect of melatonin on lung cancer cells. There are no data available on the prognostic impact of melatonin receptors MT1 and MT2 in non-small cell lung cancer (NSCLC). <i>Materials and Methods</i>: Immunohistochemical studies of MT1 and MT2 were conducted on NSCLC (N = 786) and non-malignant lung tissue (NMLT) (N = 120) using tissue microarrays. Molecular studies were performed on frozen fragments of NSCLC (N = 62; real time PCR), NMLT (N = 24) and lung cancer cell lines NCI-H1703, A549 and IMR-90 (real time PCR, western blot). <i>Results</i>: The expression of both receptors was higher in NSCLC than in NMLT. Higher MT1 and MT2 expression levels (at protein and mRNA) were noted in squamous cell carcinomas (SCC) compared to adenocarcinomas (AC). MT1 immunoexpression decreased as both the tumour size and the cancer stage increased in the whole cohort, while MT2 decreased as the cancer stage increased, with lymph node involvement (in the whole study group) and increasing malignancy grade (in SCC). Higher expression of MT2 was associated with a favorable prognosis. MT2 was an independent prognostic factor for overall survival (OS) in all analyzed NSCLC and in smoking patients. <i>Conclusions</i>: Our observations may point to the potential prognostic significance of MT2 in NSCLC. |
topic |
melatonin melatonin receptor MT1 MT2 non-small cell lung carcinoma immunohistochemistry cancer |
url |
https://www.mdpi.com/2072-6694/11/7/1001 |
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