Improved efficiency of in situ protein analysis by proximity ligation using UnFold probes
Abstract We have redesigned probes for in situ proximity ligation assay (PLA), resulting in more efficient localized detection of target proteins. In situ PLA depends on recognition of target proteins by pairs of antibody-oligonucleotide conjugates (PLA probes), which jointly give rise to DNA circle...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2018-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-018-23582-1 |