HIV Viral Load Estimation Using Hematocrit Corrected Dried Blood Spot Results on a BioMerieux NucliSENS<sup>®</sup> Platform
While reporting human immunodeficiency virus (HIV) viral load (VL) using dried blood spot (DBS) in the BioMerieux NucliSENS platform, application of the hematocrit correction factor has been suggested. In this cross-sectional study from the National Microbiology Reference Laboratory of Zimbabwe, we...
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doaj-75bcc4f343b14acb93df73389e83eaf62020-11-24T21:30:42ZengMDPI AGDiagnostics2075-44182019-07-01938610.3390/diagnostics9030086diagnostics9030086HIV Viral Load Estimation Using Hematocrit Corrected Dried Blood Spot Results on a BioMerieux NucliSENS<sup>®</sup> PlatformCharles Nyagupe0Hemant Deepak Shewade1Serge Ade2Collins Timire3Hannock Tweya4Norah Vere5Sandra Chipuka6Lucia Sisya7Hlanai Gumbo8Ezekiel Ditima9Sekesai Zinyowera10National TB and HIV/AIDS Programme, Ministry of Health and Child Care, Harare P O Box CY1122 Causeway, 00263, ZimbabweInternational Union against Tuberculosis and Lung Disease (The Union), 75006 Paris, FranceInternational Union against Tuberculosis and Lung Disease (The Union), 75006 Paris, FranceNational TB and HIV/AIDS Programme, Ministry of Health and Child Care, Harare P O Box CY1122 Causeway, 00263, ZimbabweInternational Union against Tuberculosis and Lung Disease (The Union), 75006 Paris, FranceNational TB and HIV/AIDS Programme, Ministry of Health and Child Care, Harare P O Box CY1122 Causeway, 00263, ZimbabweNational TB and HIV/AIDS Programme, Ministry of Health and Child Care, Harare P O Box CY1122 Causeway, 00263, ZimbabweNational TB and HIV/AIDS Programme, Ministry of Health and Child Care, Harare P O Box CY1122 Causeway, 00263, ZimbabweNational TB and HIV/AIDS Programme, Ministry of Health and Child Care, Harare P O Box CY1122 Causeway, 00263, ZimbabweNational TB and HIV/AIDS Programme, Ministry of Health and Child Care, Harare P O Box CY1122 Causeway, 00263, ZimbabweNational TB and HIV/AIDS Programme, Ministry of Health and Child Care, Harare P O Box CY1122 Causeway, 00263, ZimbabweWhile reporting human immunodeficiency virus (HIV) viral load (VL) using dried blood spot (DBS) in the BioMerieux NucliSENS platform, application of the hematocrit correction factor has been suggested. In this cross-sectional study from the National Microbiology Reference Laboratory of Zimbabwe, we assessed whether hematocrit correction (individual and/or mean) in DBS results improved the correlation with plasma VL and prediction of VL non-suppression (≥1000 copies per ml in plasma). Of 517 specimens during August−December 2018, 65(12.6%) had non-suppressed plasma VL results. The hematocrit correction factor ranged from 1.3 to 2.0 with a mean of 1.6, standard deviation (SD: 1.5, 1.7). The intraclass correlation (ICC) for mean (0.859, 95% CI: 0.834, 0.880) and individual (0.809, 95% CI: 0.777, 0.837) hematocrit corrected DBS results were not significantly different. The uncorrected DBS results had a significantly lower ICC (0.640, 95% CI: 0.586, 0.688) when compared to corrected DBS results. There were no significant differences in validity, predictive values, and areas under the receiver operating characteristics curves for all three DBS results when predicting VL non-suppression. To conclude, hematocrit correction of DBS VL results improved agreement with the plasma results but did not improve prediction of VL non-suppression. The results were not significantly different for individual and mean corrected results.https://www.mdpi.com/2075-4418/9/3/86viral loaddried blood spotAntiretroviral therapy (ART) monitoringhuman immunodeficiency virushematocrit |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Charles Nyagupe Hemant Deepak Shewade Serge Ade Collins Timire Hannock Tweya Norah Vere Sandra Chipuka Lucia Sisya Hlanai Gumbo Ezekiel Ditima Sekesai Zinyowera |
spellingShingle |
Charles Nyagupe Hemant Deepak Shewade Serge Ade Collins Timire Hannock Tweya Norah Vere Sandra Chipuka Lucia Sisya Hlanai Gumbo Ezekiel Ditima Sekesai Zinyowera HIV Viral Load Estimation Using Hematocrit Corrected Dried Blood Spot Results on a BioMerieux NucliSENS<sup>®</sup> Platform Diagnostics viral load dried blood spot Antiretroviral therapy (ART) monitoring human immunodeficiency virus hematocrit |
author_facet |
Charles Nyagupe Hemant Deepak Shewade Serge Ade Collins Timire Hannock Tweya Norah Vere Sandra Chipuka Lucia Sisya Hlanai Gumbo Ezekiel Ditima Sekesai Zinyowera |
author_sort |
Charles Nyagupe |
title |
HIV Viral Load Estimation Using Hematocrit Corrected Dried Blood Spot Results on a BioMerieux NucliSENS<sup>®</sup> Platform |
title_short |
HIV Viral Load Estimation Using Hematocrit Corrected Dried Blood Spot Results on a BioMerieux NucliSENS<sup>®</sup> Platform |
title_full |
HIV Viral Load Estimation Using Hematocrit Corrected Dried Blood Spot Results on a BioMerieux NucliSENS<sup>®</sup> Platform |
title_fullStr |
HIV Viral Load Estimation Using Hematocrit Corrected Dried Blood Spot Results on a BioMerieux NucliSENS<sup>®</sup> Platform |
title_full_unstemmed |
HIV Viral Load Estimation Using Hematocrit Corrected Dried Blood Spot Results on a BioMerieux NucliSENS<sup>®</sup> Platform |
title_sort |
hiv viral load estimation using hematocrit corrected dried blood spot results on a biomerieux nuclisens<sup>®</sup> platform |
publisher |
MDPI AG |
series |
Diagnostics |
issn |
2075-4418 |
publishDate |
2019-07-01 |
description |
While reporting human immunodeficiency virus (HIV) viral load (VL) using dried blood spot (DBS) in the BioMerieux NucliSENS platform, application of the hematocrit correction factor has been suggested. In this cross-sectional study from the National Microbiology Reference Laboratory of Zimbabwe, we assessed whether hematocrit correction (individual and/or mean) in DBS results improved the correlation with plasma VL and prediction of VL non-suppression (≥1000 copies per ml in plasma). Of 517 specimens during August−December 2018, 65(12.6%) had non-suppressed plasma VL results. The hematocrit correction factor ranged from 1.3 to 2.0 with a mean of 1.6, standard deviation (SD: 1.5, 1.7). The intraclass correlation (ICC) for mean (0.859, 95% CI: 0.834, 0.880) and individual (0.809, 95% CI: 0.777, 0.837) hematocrit corrected DBS results were not significantly different. The uncorrected DBS results had a significantly lower ICC (0.640, 95% CI: 0.586, 0.688) when compared to corrected DBS results. There were no significant differences in validity, predictive values, and areas under the receiver operating characteristics curves for all three DBS results when predicting VL non-suppression. To conclude, hematocrit correction of DBS VL results improved agreement with the plasma results but did not improve prediction of VL non-suppression. The results were not significantly different for individual and mean corrected results. |
topic |
viral load dried blood spot Antiretroviral therapy (ART) monitoring human immunodeficiency virus hematocrit |
url |
https://www.mdpi.com/2075-4418/9/3/86 |
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