The challenges with the validation of research antibodies [version 1; referees: 2 approved]

This article further discusses the reproducibility crisis in biomedical science and how poor conduct of commercial antibodies contribute to this. In addition, the way quality data are presented on product sheets by antibody vendors is scrutinized. The article proposes that there is a distinction bet...

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Bibliographic Details
Main Author: Jan L.A. Voskuil
Format: Article
Language:English
Published: F1000 Research Ltd 2017-02-01
Series:F1000Research
Subjects:
Online Access:https://f1000research.com/articles/6-161/v1
Description
Summary:This article further discusses the reproducibility crisis in biomedical science and how poor conduct of commercial antibodies contribute to this. In addition, the way quality data are presented on product sheets by antibody vendors is scrutinized. The article proposes that there is a distinction between testing data and validation data, and special attention is asked for consistency between batches and aliquots. Moreover, the article separates the specifics, such as formulation, antigen and price, from the specifics on performance. Finally, a two-tier approach is discussed, enabling scientists to anticipate how an antibody is likely to perform when repeated purchases are required.
ISSN:2046-1402