De novo Transcriptome Assembly and Comparative Analysis Highlight the Primary Mechanism Regulating the Response to Selenium Stimuli in Oats (Avena sativa L.)
Selenium is an essential microelement for humans and animals. The specific processing technique of oats can maximize the preservation of its nutrients. In this study, to understand the genetic response of oats in a high-selenium environment, oats were treated with sodium selenate for 24 h, and trans...
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2021-06-01
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doaj-761dce77b0134d878d9cc1dbca9c50072021-06-18T06:22:33ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-06-011210.3389/fpls.2021.625520625520De novo Transcriptome Assembly and Comparative Analysis Highlight the Primary Mechanism Regulating the Response to Selenium Stimuli in Oats (Avena sativa L.)Tao Liu0Tao Liu1Tao Liu2Xiaoting Liu3Rangrang Zhou4Rangrang Zhou5Rangrang Zhou6Hong Chen7Hong Chen8Hong Chen9Huaigang Zhang10Huaigang Zhang11Huaigang Zhang12Bo Zhang13Bo Zhang14Bo Zhang15Key Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaQinghai Province Key Laboratory of Crop Molecular Breeding, Xining, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaXining No. 8 Junior High School, Xining, ChinaKey Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaQinghai Province Key Laboratory of Crop Molecular Breeding, Xining, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaQinghai Province Key Laboratory of Crop Molecular Breeding, Xining, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaQinghai Province Key Laboratory of Crop Molecular Breeding, Xining, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Adaptation and Evolution of Plateau Biota (AEPB), Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, ChinaQinghai Province Key Laboratory of Crop Molecular Breeding, Xining, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaSelenium is an essential microelement for humans and animals. The specific processing technique of oats can maximize the preservation of its nutrients. In this study, to understand the genetic response of oats in a high-selenium environment, oats were treated with sodium selenate for 24 h, and transcriptome analysis was performed. A total of 211,485,930 clean reads composing 31.30 Gb of clean data were retained for four samples. After assembly, 186,035 unigenes with an average length of 727 bp were generated, and the N50 length was 1,149 bp. Compared with that in the control group, the expression of 7,226 unigenes in the treatment group was upregulated, and 2,618 unigenes were downregulated. Based on the sulfur assimilation pathway and selenocompound metabolic pathway, a total of 27 unigenes related to selenate metabolism were identified. Among them, the expression of both key genes APS (ATP sulfurylase) and APR (adenosine 5′-phosphosulfate reductase) was upregulated more than 1,000-fold under selenate treatment, while that of CBL (cystathionine-β-synthase) was upregulated 3.12-fold. Based on the transcriptome analysis, we suspect that the high-affinity sulfur transporter Sultr1;2 plays a key role in selenate uptake in oats. A preliminary regulatory mechanism explains the oat response to selenate treatment was ultimately proposed based on the transcriptome analysis and previous research.https://www.frontiersin.org/articles/10.3389/fpls.2021.625520/fullseleniumoatstranscriptome analysisselenoproteinsselenium metabolism |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tao Liu Tao Liu Tao Liu Xiaoting Liu Rangrang Zhou Rangrang Zhou Rangrang Zhou Hong Chen Hong Chen Hong Chen Huaigang Zhang Huaigang Zhang Huaigang Zhang Bo Zhang Bo Zhang Bo Zhang |
spellingShingle |
Tao Liu Tao Liu Tao Liu Xiaoting Liu Rangrang Zhou Rangrang Zhou Rangrang Zhou Hong Chen Hong Chen Hong Chen Huaigang Zhang Huaigang Zhang Huaigang Zhang Bo Zhang Bo Zhang Bo Zhang De novo Transcriptome Assembly and Comparative Analysis Highlight the Primary Mechanism Regulating the Response to Selenium Stimuli in Oats (Avena sativa L.) Frontiers in Plant Science selenium oats transcriptome analysis selenoproteins selenium metabolism |
author_facet |
Tao Liu Tao Liu Tao Liu Xiaoting Liu Rangrang Zhou Rangrang Zhou Rangrang Zhou Hong Chen Hong Chen Hong Chen Huaigang Zhang Huaigang Zhang Huaigang Zhang Bo Zhang Bo Zhang Bo Zhang |
author_sort |
Tao Liu |
title |
De novo Transcriptome Assembly and Comparative Analysis Highlight the Primary Mechanism Regulating the Response to Selenium Stimuli in Oats (Avena sativa L.) |
title_short |
De novo Transcriptome Assembly and Comparative Analysis Highlight the Primary Mechanism Regulating the Response to Selenium Stimuli in Oats (Avena sativa L.) |
title_full |
De novo Transcriptome Assembly and Comparative Analysis Highlight the Primary Mechanism Regulating the Response to Selenium Stimuli in Oats (Avena sativa L.) |
title_fullStr |
De novo Transcriptome Assembly and Comparative Analysis Highlight the Primary Mechanism Regulating the Response to Selenium Stimuli in Oats (Avena sativa L.) |
title_full_unstemmed |
De novo Transcriptome Assembly and Comparative Analysis Highlight the Primary Mechanism Regulating the Response to Selenium Stimuli in Oats (Avena sativa L.) |
title_sort |
de novo transcriptome assembly and comparative analysis highlight the primary mechanism regulating the response to selenium stimuli in oats (avena sativa l.) |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2021-06-01 |
description |
Selenium is an essential microelement for humans and animals. The specific processing technique of oats can maximize the preservation of its nutrients. In this study, to understand the genetic response of oats in a high-selenium environment, oats were treated with sodium selenate for 24 h, and transcriptome analysis was performed. A total of 211,485,930 clean reads composing 31.30 Gb of clean data were retained for four samples. After assembly, 186,035 unigenes with an average length of 727 bp were generated, and the N50 length was 1,149 bp. Compared with that in the control group, the expression of 7,226 unigenes in the treatment group was upregulated, and 2,618 unigenes were downregulated. Based on the sulfur assimilation pathway and selenocompound metabolic pathway, a total of 27 unigenes related to selenate metabolism were identified. Among them, the expression of both key genes APS (ATP sulfurylase) and APR (adenosine 5′-phosphosulfate reductase) was upregulated more than 1,000-fold under selenate treatment, while that of CBL (cystathionine-β-synthase) was upregulated 3.12-fold. Based on the transcriptome analysis, we suspect that the high-affinity sulfur transporter Sultr1;2 plays a key role in selenate uptake in oats. A preliminary regulatory mechanism explains the oat response to selenate treatment was ultimately proposed based on the transcriptome analysis and previous research. |
topic |
selenium oats transcriptome analysis selenoproteins selenium metabolism |
url |
https://www.frontiersin.org/articles/10.3389/fpls.2021.625520/full |
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