CD161 Expression Defines a Th1/Th17 Polyfunctional Subset of Resident Memory T Lymphocytes in Bronchoalveolar Cells.
Alveolar resident memory T cells (T(RM)) comprise a currently uncharacterized mixture of cell subpopulations. The CD3(+)CD161(+) T cell subpopulation resides in the liver, intestine and skin, but it has the capacity for tissue migration; however, the presence of resident CD3(+)CD161(+) T cells in th...
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doaj-762ab7da2f8b4de2bfb247137acfbdef2020-11-25T02:23:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01104e012359110.1371/journal.pone.0123591CD161 Expression Defines a Th1/Th17 Polyfunctional Subset of Resident Memory T Lymphocytes in Bronchoalveolar Cells.Yolanda GonzalezMaría Teresa HerreraEsmeralda JuárezMiguel Angel Salazar-LezamaKaren BobadillaMartha TorresAlveolar resident memory T cells (T(RM)) comprise a currently uncharacterized mixture of cell subpopulations. The CD3(+)CD161(+) T cell subpopulation resides in the liver, intestine and skin, but it has the capacity for tissue migration; however, the presence of resident CD3(+)CD161(+) T cells in the bronchoalveolar space under normal conditions has not been reported. Bronchoalveolar cells (BACs) from healthy volunteers were evaluated and found that 8.6% (range 2.5%-21%) of these cells were CD3(+) T lymphocytes. Within the CD3(+) population, 4.6% of the cells (2.1-11.3) expressed CD161 on the cell surface, and 74.2% of the CD161(+)CD3(+) T cells expressed CD45RO. The number of CD3(+)CD161(+) T cells was significantly lower in the bronchoalveolar space than in the blood (4.6% of BACs vs 8.4% of peripheral blood mononuclear cells (PBMCs); P<0.05). We also found that 2.17% of CD4(+) T lymphocytes and 1.52% of CD8(+) T lymphocytes expressed CD161. Twenty-two percent of the alveolar CD3(+)CD161(+) T lymphocytes produced cytokines upon stimulation by PMA plus ionomycin, and significantly more interferon gamma (IFN-γ) was produced compared with other cytokines (P = 0.05). Most alveolar CD3(+)CD161(+) T cells produced interleukin-17 (IL-17) and IFN-γ simultaneously, and the percentage of these cells was significantly higher than the percentage of CD3(+)CD161- T cells. Moreover, the percentage of alveolar CD3(+)CD161(+) T lymphocytes that produced IFN-γ/IL-17 was significantly higher than those in the peripheral blood (p<0.05). In conclusion, Th1/Th17-CD3(+)CD161(+) TRM could contribute to compartment-specific immune responses in the lung.http://europepmc.org/articles/PMC4408072?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yolanda Gonzalez María Teresa Herrera Esmeralda Juárez Miguel Angel Salazar-Lezama Karen Bobadilla Martha Torres |
spellingShingle |
Yolanda Gonzalez María Teresa Herrera Esmeralda Juárez Miguel Angel Salazar-Lezama Karen Bobadilla Martha Torres CD161 Expression Defines a Th1/Th17 Polyfunctional Subset of Resident Memory T Lymphocytes in Bronchoalveolar Cells. PLoS ONE |
author_facet |
Yolanda Gonzalez María Teresa Herrera Esmeralda Juárez Miguel Angel Salazar-Lezama Karen Bobadilla Martha Torres |
author_sort |
Yolanda Gonzalez |
title |
CD161 Expression Defines a Th1/Th17 Polyfunctional Subset of Resident Memory T Lymphocytes in Bronchoalveolar Cells. |
title_short |
CD161 Expression Defines a Th1/Th17 Polyfunctional Subset of Resident Memory T Lymphocytes in Bronchoalveolar Cells. |
title_full |
CD161 Expression Defines a Th1/Th17 Polyfunctional Subset of Resident Memory T Lymphocytes in Bronchoalveolar Cells. |
title_fullStr |
CD161 Expression Defines a Th1/Th17 Polyfunctional Subset of Resident Memory T Lymphocytes in Bronchoalveolar Cells. |
title_full_unstemmed |
CD161 Expression Defines a Th1/Th17 Polyfunctional Subset of Resident Memory T Lymphocytes in Bronchoalveolar Cells. |
title_sort |
cd161 expression defines a th1/th17 polyfunctional subset of resident memory t lymphocytes in bronchoalveolar cells. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2015-01-01 |
description |
Alveolar resident memory T cells (T(RM)) comprise a currently uncharacterized mixture of cell subpopulations. The CD3(+)CD161(+) T cell subpopulation resides in the liver, intestine and skin, but it has the capacity for tissue migration; however, the presence of resident CD3(+)CD161(+) T cells in the bronchoalveolar space under normal conditions has not been reported. Bronchoalveolar cells (BACs) from healthy volunteers were evaluated and found that 8.6% (range 2.5%-21%) of these cells were CD3(+) T lymphocytes. Within the CD3(+) population, 4.6% of the cells (2.1-11.3) expressed CD161 on the cell surface, and 74.2% of the CD161(+)CD3(+) T cells expressed CD45RO. The number of CD3(+)CD161(+) T cells was significantly lower in the bronchoalveolar space than in the blood (4.6% of BACs vs 8.4% of peripheral blood mononuclear cells (PBMCs); P<0.05). We also found that 2.17% of CD4(+) T lymphocytes and 1.52% of CD8(+) T lymphocytes expressed CD161. Twenty-two percent of the alveolar CD3(+)CD161(+) T lymphocytes produced cytokines upon stimulation by PMA plus ionomycin, and significantly more interferon gamma (IFN-γ) was produced compared with other cytokines (P = 0.05). Most alveolar CD3(+)CD161(+) T cells produced interleukin-17 (IL-17) and IFN-γ simultaneously, and the percentage of these cells was significantly higher than the percentage of CD3(+)CD161- T cells. Moreover, the percentage of alveolar CD3(+)CD161(+) T lymphocytes that produced IFN-γ/IL-17 was significantly higher than those in the peripheral blood (p<0.05). In conclusion, Th1/Th17-CD3(+)CD161(+) TRM could contribute to compartment-specific immune responses in the lung. |
url |
http://europepmc.org/articles/PMC4408072?pdf=render |
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