Detection and Molecular Characterization of Cryptosporidium Species in Recreational Waters of Chaharmahal va Bakhtiyari Province of Iran Using Nested-PCR-RFLP

• Main Authors: K Manouchehri Naeini, M Asadi, M Hashemzade Chaleshtori
• Format: Article
• Language: English
• Published: Tehran University of Medical Sciences 2011-03-01
• Series: Iranian Journal of Parasitology
• Subjects: Recreational Waters; Cryptosporidium; Genotyping; Nested-PCR-RFLP; Iran
• Online Access: http://journals.tums.ac.ir/upload_files/pdf/17732.pdf

Background: The aim of this study was to detect and characterize Cryptosporidium spp. in water sam­ples collected from recreational ponds of Chaharmahal va Bakhtiyari Province of Iran .Meth­ods: Thirty water samples were collected from November 2009 to May 2010. Each sample con­tained 10 liters of water. We used the SSU rRNA-based PCR-RFLP technique.Results: Out of thirty samples examined, 6 (20%) were positive for different Cryptosporidium spp. Restriction pattern analysis showed that C. parvum has been the most prevalent genotype, fol­lowed by C. hominis and C. canis , respectively. In this area, the higher prevalence of C. par­vum compared with other genotypes is consistent with the distribution of cattle.Conclusion: Farm animals, particularly cattle are the main source of cryptosporidial contamina­tion for recreational waters in this area.