AZF Microdeletions in Human Semen Infected with Bacteria

• Main Authors: Hayfa H Hassani, Zainab SH Khalaf, Ehaim J Samarie, Mohammed A Ibrahim
• Format: Article
• Language: English
• Published: Light House Polyclinic Mangalore 2011-11-01
• Series: Online Journal of Health & Allied Sciences
• Subjects: Male infertility; Yq microdeletion; Chlamydia trachomatis; Neisseria gonorrheae
• Online Access: http://www.ojhas.org/issue39/2011-3-8.htm

Bacterial infections are associated with infertility in men. This study was aimed to investigate microdeletions on Yq chromosome in semen infected with bacteria by using bacteriological, biochemical, and serological assays. The investigation showed that 107 of 300 (84.80%) semen samples collected from infertile men with primary or secondary infertility were infected with different species of bacteria. Chlamydia trachomatis and Neisseria gonorrheae were the most frequently diagnosed bacteria in the infected semen samples. The percentages of infections of semen samples with C. trachomatis and N. gonorrhea were 42.31% and 35.28% respectively. Genomic DNA from each semen sample infected with predominant bacteria was analyzed for AZF deletions by using multiplex PCR. Different patterns of AZF microdeletions were obtained. It can be concluded that sexually transmitted bacteria may contribute in microdeletions of Yq chromosome by indirectly producing reactive oxygen species and causing gene defect in AZF regions.