Results of Dynamic Contrast-Enhanced Ultrasound Correlate With Treatment Outcome in Canine Neoplasia Treated With Electrochemotherapy and Interleukin-12 Plasmid Electrotransfer
Electrochemotherapy (ECT) and/or gene electrotransfer of plasmid DNA encoding interleukin-12 (GET pIL-12) are effective treatments for canine cutaneous, subcutaneous, and maxillofacial tumors. Despite the clinical efficacy of the combined treatments of ECT and GET, data on parameters that might pred...
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2021-05-01
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doaj-763ca69360e14c0fbe3ca5efe67077e32021-05-20T06:03:16ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-05-01810.3389/fvets.2021.679073679073Results of Dynamic Contrast-Enhanced Ultrasound Correlate With Treatment Outcome in Canine Neoplasia Treated With Electrochemotherapy and Interleukin-12 Plasmid ElectrotransferMaja Brloznik0Simona Kranjc Brezar1Simona Kranjc Brezar2Nina Boc3Tanja Knific4Maja Cemazar5Maja Cemazar6Nina Milevoj7Gregor Sersa8Gregor Sersa9Natasa Tozon10Darja Pavlin11Veterinary Faculty, Small Animal Clinic, University of Ljubljana, Ljubljana, SloveniaDepartment of Experimental Oncology, Institute of Oncology Ljubljana, Ljubljana, SloveniaFaculty of Medicine, University of Ljubljana, Ljubljana, SloveniaDepartment of Radiology, Institute of Oncology Ljubljana, Ljubljana, SloveniaInstitute of Food Safety, Feed and Environment, Veterinary Faculty, University of Ljubljana, Ljubljana, SloveniaDepartment of Experimental Oncology, Institute of Oncology Ljubljana, Ljubljana, SloveniaFaculty of Health Sciences, University of Primorska, Izola, SloveniaVeterinary Faculty, Small Animal Clinic, University of Ljubljana, Ljubljana, SloveniaDepartment of Experimental Oncology, Institute of Oncology Ljubljana, Ljubljana, SloveniaFaculty of Health Sciences, University of Ljubljana, Ljubljana, SloveniaVeterinary Faculty, Small Animal Clinic, University of Ljubljana, Ljubljana, SloveniaVeterinary Faculty, Small Animal Clinic, University of Ljubljana, Ljubljana, SloveniaElectrochemotherapy (ECT) and/or gene electrotransfer of plasmid DNA encoding interleukin-12 (GET pIL-12) are effective treatments for canine cutaneous, subcutaneous, and maxillofacial tumors. Despite the clinical efficacy of the combined treatments of ECT and GET, data on parameters that might predict the outcome of the treatments are still lacking. This study aimed to investigate whether dynamic contrast-enhanced ultrasound (DCE-US) results of subcutaneous tumors differ between tumors with complete response (CR) and tumors without complete response (non-CR) in dogs treated with ECT and GET pIL-12. Eight dogs with a total of 12 tumor nodules treated with ECT and GET pIL-12 were included. DCE-US examinations were performed in all animals before and immediately after therapy as well as 8 h and 1, 3, and 7 days later. Clinical follow-up examinations were performed 7 and 14 days, 1 and 6 months, and 1 year after treatment. Numerous significant differences in DCE-US parameters were noted between tumors with CR and non-CR tumors; perfusion and perfusion heterogeneity were lower in CR tumors than in non-CR tumors. Therefore, studies with larger numbers of patients are needed to investigate whether DCE-US results can be used to predict treatment outcomes and to make effective decisions about the need for repeated therapy or different treatment combinations in individual patients.https://www.frontiersin.org/articles/10.3389/fvets.2021.679073/fulldogcontrast-enhanced ultrasonographyelectrochemotherapyimmunotherapygene electrotransferbleomycin |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maja Brloznik Simona Kranjc Brezar Simona Kranjc Brezar Nina Boc Tanja Knific Maja Cemazar Maja Cemazar Nina Milevoj Gregor Sersa Gregor Sersa Natasa Tozon Darja Pavlin |
spellingShingle |
Maja Brloznik Simona Kranjc Brezar Simona Kranjc Brezar Nina Boc Tanja Knific Maja Cemazar Maja Cemazar Nina Milevoj Gregor Sersa Gregor Sersa Natasa Tozon Darja Pavlin Results of Dynamic Contrast-Enhanced Ultrasound Correlate With Treatment Outcome in Canine Neoplasia Treated With Electrochemotherapy and Interleukin-12 Plasmid Electrotransfer Frontiers in Veterinary Science dog contrast-enhanced ultrasonography electrochemotherapy immunotherapy gene electrotransfer bleomycin |
author_facet |
Maja Brloznik Simona Kranjc Brezar Simona Kranjc Brezar Nina Boc Tanja Knific Maja Cemazar Maja Cemazar Nina Milevoj Gregor Sersa Gregor Sersa Natasa Tozon Darja Pavlin |
author_sort |
Maja Brloznik |
title |
Results of Dynamic Contrast-Enhanced Ultrasound Correlate With Treatment Outcome in Canine Neoplasia Treated With Electrochemotherapy and Interleukin-12 Plasmid Electrotransfer |
title_short |
Results of Dynamic Contrast-Enhanced Ultrasound Correlate With Treatment Outcome in Canine Neoplasia Treated With Electrochemotherapy and Interleukin-12 Plasmid Electrotransfer |
title_full |
Results of Dynamic Contrast-Enhanced Ultrasound Correlate With Treatment Outcome in Canine Neoplasia Treated With Electrochemotherapy and Interleukin-12 Plasmid Electrotransfer |
title_fullStr |
Results of Dynamic Contrast-Enhanced Ultrasound Correlate With Treatment Outcome in Canine Neoplasia Treated With Electrochemotherapy and Interleukin-12 Plasmid Electrotransfer |
title_full_unstemmed |
Results of Dynamic Contrast-Enhanced Ultrasound Correlate With Treatment Outcome in Canine Neoplasia Treated With Electrochemotherapy and Interleukin-12 Plasmid Electrotransfer |
title_sort |
results of dynamic contrast-enhanced ultrasound correlate with treatment outcome in canine neoplasia treated with electrochemotherapy and interleukin-12 plasmid electrotransfer |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Veterinary Science |
issn |
2297-1769 |
publishDate |
2021-05-01 |
description |
Electrochemotherapy (ECT) and/or gene electrotransfer of plasmid DNA encoding interleukin-12 (GET pIL-12) are effective treatments for canine cutaneous, subcutaneous, and maxillofacial tumors. Despite the clinical efficacy of the combined treatments of ECT and GET, data on parameters that might predict the outcome of the treatments are still lacking. This study aimed to investigate whether dynamic contrast-enhanced ultrasound (DCE-US) results of subcutaneous tumors differ between tumors with complete response (CR) and tumors without complete response (non-CR) in dogs treated with ECT and GET pIL-12. Eight dogs with a total of 12 tumor nodules treated with ECT and GET pIL-12 were included. DCE-US examinations were performed in all animals before and immediately after therapy as well as 8 h and 1, 3, and 7 days later. Clinical follow-up examinations were performed 7 and 14 days, 1 and 6 months, and 1 year after treatment. Numerous significant differences in DCE-US parameters were noted between tumors with CR and non-CR tumors; perfusion and perfusion heterogeneity were lower in CR tumors than in non-CR tumors. Therefore, studies with larger numbers of patients are needed to investigate whether DCE-US results can be used to predict treatment outcomes and to make effective decisions about the need for repeated therapy or different treatment combinations in individual patients. |
topic |
dog contrast-enhanced ultrasonography electrochemotherapy immunotherapy gene electrotransfer bleomycin |
url |
https://www.frontiersin.org/articles/10.3389/fvets.2021.679073/full |
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