DETERMINATION OF ANTIBIOTICS (CHLORAMPHENICOL AND TETRACYCLINE) IN FOODS WITH DIFFERENT MATRICES
A food product is a multicomponent system containing proteins, fats, carbohydrates. Determination of antibiotics in this case is complicated by the influence of interfering components of different nature. Methods for determining antibiotics (chloramphenicol and tetracycline) in foods with multicompo...
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Kemerovo State University
2015-09-01
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doaj-763ed9858b1e4becb57907415c7458982020-11-25T02:18:54ZrusKemerovo State UniversityТехника и технология пищевых производств2074-94142313-17482015-09-01383146152DETERMINATION OF ANTIBIOTICS (CHLORAMPHENICOL AND TETRACYCLINE) IN FOODS WITH DIFFERENT MATRICESSokolova L.I.0Belyustova K.O.1Privar Y.O.2Shapkin N.P.3Razov V.I.4Far Eastern Federal UniversityFar Eastern Federal UniversityFar Eastern Federal UniversityFar Eastern Federal UniversityFar Eastern Federal UniversityA food product is a multicomponent system containing proteins, fats, carbohydrates. Determination of antibiotics in this case is complicated by the influence of interfering components of different nature. Methods for determining antibiotics (chloramphenicol and tetracycline) in foods with multicomponent matrices (lipid-protein, lipid-carbohydrate) have been proposed. Techniques include antibiotics extraction using various solvents, purification of extracts and concentration of antibiotics using natural sorbents (activated carbon and silica-alumina), followed by eluting of certain components with ethanol and analyzing by high performance liquid chromatography (HPLC) with UV detection. The content of antibiotics was determined in products purchased in retail stores in Vladivostok: lime honey produced in the farms of the Primorsky Territory and the Republic of Bashkortostan, hawthorn honey produced in the farms of the Republic of Bashkortostan, and chicken liver produced in the poultry farms of the Primorsky Territory. The extraction degrees of chloramphenicol and tetracycline from honey and chloramphenicol from chicken liver have been determined by the «added-found» method. The extraction degree of chloramphenicol from honey was 90%, from chicken liver - 78%. The extraction degree of tetracycline from honey samples was 82%. The content of chloramphenicol and tetracycline in some honey samples and chloramphenicol in chicken liver samples purchased in the retail network was determined. Tetracycline and chloramphenicol were found in 2 samples of lime honey with antibiotics content of 0.052 and 0.020 mg/kg, respectively. Chicken liver samples contained from 0.007 to 0.01 mg/kg of chloramphenicol.http://fptt.ru/stories/archive/38/23.pdfchloramphenicoltetracyclineextractionadsorptionsorbentconcentrationhigh-efficient liquid chromatography |
collection |
DOAJ |
language |
Russian |
format |
Article |
sources |
DOAJ |
author |
Sokolova L.I. Belyustova K.O. Privar Y.O. Shapkin N.P. Razov V.I. |
spellingShingle |
Sokolova L.I. Belyustova K.O. Privar Y.O. Shapkin N.P. Razov V.I. DETERMINATION OF ANTIBIOTICS (CHLORAMPHENICOL AND TETRACYCLINE) IN FOODS WITH DIFFERENT MATRICES Техника и технология пищевых производств chloramphenicol tetracycline extraction adsorption sorbent concentration high-efficient liquid chromatography |
author_facet |
Sokolova L.I. Belyustova K.O. Privar Y.O. Shapkin N.P. Razov V.I. |
author_sort |
Sokolova L.I. |
title |
DETERMINATION OF ANTIBIOTICS (CHLORAMPHENICOL AND TETRACYCLINE) IN FOODS WITH DIFFERENT MATRICES |
title_short |
DETERMINATION OF ANTIBIOTICS (CHLORAMPHENICOL AND TETRACYCLINE) IN FOODS WITH DIFFERENT MATRICES |
title_full |
DETERMINATION OF ANTIBIOTICS (CHLORAMPHENICOL AND TETRACYCLINE) IN FOODS WITH DIFFERENT MATRICES |
title_fullStr |
DETERMINATION OF ANTIBIOTICS (CHLORAMPHENICOL AND TETRACYCLINE) IN FOODS WITH DIFFERENT MATRICES |
title_full_unstemmed |
DETERMINATION OF ANTIBIOTICS (CHLORAMPHENICOL AND TETRACYCLINE) IN FOODS WITH DIFFERENT MATRICES |
title_sort |
determination of antibiotics (chloramphenicol and tetracycline) in foods with different matrices |
publisher |
Kemerovo State University |
series |
Техника и технология пищевых производств |
issn |
2074-9414 2313-1748 |
publishDate |
2015-09-01 |
description |
A food product is a multicomponent system containing proteins, fats, carbohydrates. Determination of antibiotics in this case is complicated by the influence of interfering components of different nature. Methods for determining antibiotics (chloramphenicol and tetracycline) in foods with multicomponent matrices (lipid-protein, lipid-carbohydrate) have been proposed. Techniques include antibiotics extraction using various solvents, purification of extracts and concentration of antibiotics using natural sorbents (activated carbon and silica-alumina), followed by eluting of certain components with ethanol and analyzing by high performance liquid chromatography (HPLC) with UV detection. The content of antibiotics was determined in products purchased in retail stores in Vladivostok: lime honey produced in the farms of the Primorsky Territory and the Republic of Bashkortostan, hawthorn honey produced in the farms of the Republic of Bashkortostan, and chicken liver produced in the poultry farms of the Primorsky Territory. The extraction degrees of chloramphenicol and tetracycline from honey and chloramphenicol from chicken liver have been determined by the «added-found» method. The extraction degree of chloramphenicol from honey was 90%, from chicken liver - 78%. The extraction degree of tetracycline from honey samples was 82%. The content of chloramphenicol and tetracycline in some honey samples and chloramphenicol in chicken liver samples purchased in the retail network was determined. Tetracycline and chloramphenicol were found in 2 samples of lime honey with antibiotics content of 0.052 and 0.020 mg/kg, respectively. Chicken liver samples contained from 0.007 to 0.01 mg/kg of chloramphenicol. |
topic |
chloramphenicol tetracycline extraction adsorption sorbent concentration high-efficient liquid chromatography |
url |
http://fptt.ru/stories/archive/38/23.pdf |
work_keys_str_mv |
AT sokolovali determinationofantibioticschloramphenicolandtetracyclineinfoodswithdifferentmatrices AT belyustovako determinationofantibioticschloramphenicolandtetracyclineinfoodswithdifferentmatrices AT privaryo determinationofantibioticschloramphenicolandtetracyclineinfoodswithdifferentmatrices AT shapkinnp determinationofantibioticschloramphenicolandtetracyclineinfoodswithdifferentmatrices AT razovvi determinationofantibioticschloramphenicolandtetracyclineinfoodswithdifferentmatrices |
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1724880013158776832 |