Study on the Prevalence of Occult Hepatitis B Virus Infection in Patients Undergoing Hemodialysis
Occult hepatitis B (OBI) is a major challenging clinical entity characterized by the absence of hepatitis B surface antigen (HBsAg). The persistence of OBI may progress to fibrosis, cirrhosis, and hepatocellular carcinoma. This study was aimed to investigate the prevalence of OBI among HD patients....
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Tehran University of Medical Sciences
2020-07-01
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doaj-7640cfc4157c4514a917852f2dfc2a8b2020-11-25T02:50:32ZengTehran University of Medical SciencesActa Medica Iranica0044-60251735-96942020-07-0158410.18502/acta.v58i4.3923Study on the Prevalence of Occult Hepatitis B Virus Infection in Patients Undergoing HemodialysisShahab Mahmoudvand0Somayeh Shokri1Habibollah Mirzaei2Manoochehr Makvandi3Ali Teimoori4Niloofar Neisi5Roya Pirmoradi6Ali Ramazani7Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. AND Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. AND Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. AND Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. AND Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. AND Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. AND Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. AND Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. AND Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Occult hepatitis B (OBI) is a major challenging clinical entity characterized by the absence of hepatitis B surface antigen (HBsAg). The persistence of OBI may progress to fibrosis, cirrhosis, and hepatocellular carcinoma. This study was aimed to investigate the prevalence of OBI among HD patients. In the present cross-sectional study, 89 sera samples of hemodialysis individuals were tested for HBsAg and HBc-IgG by Enzyme-linked Immunosorbent Assay (ELISA). In addition, the HBV DNA has tested in sera and peripheral blood mononuclear cell (PBMC) samples by nested-PCR. Out of 89 patients, 51(57.3%) were males, and 38 (42.7%) females. The ages ranged from 24 to 90 years (with a mean of 57.5±1.37 years). All the sera samples had normal levels of Aspartate Aminotransferase (AST) and Alanine Transaminase (ALT) but had high levels of Creatinine (Cr) (6.9±2.17) and Blood Urea Nitrogen (BUN) (61.83±2.03). 2/89 (2.2%) sera samples were positive for both HBsAg and HBc-IgG test; in addition, HBV DNA was detected in both sera and their PBMC samples. The sera of 15/89 (16.85%) were only positive for the HBc-IgG test, including 10/51 (19.6%) males and 5/38 (13.2%) females (P=0.5). The high 16.85% prevalence OBI has been found among HD patients. To manage OBI infection, screening of HBV DNA should be implemented for HD patients by sensitive molecular means such as nested-PCR and real-time PCR. https://acta.tums.ac.ir/index.php/acta/article/view/8206Occult hepatitis BHemodialysisEnzyme-linked immunosorbent assay (ELISA)Nested polymerase chain reaction (Nested-PCR) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shahab Mahmoudvand Somayeh Shokri Habibollah Mirzaei Manoochehr Makvandi Ali Teimoori Niloofar Neisi Roya Pirmoradi Ali Ramazani |
spellingShingle |
Shahab Mahmoudvand Somayeh Shokri Habibollah Mirzaei Manoochehr Makvandi Ali Teimoori Niloofar Neisi Roya Pirmoradi Ali Ramazani Study on the Prevalence of Occult Hepatitis B Virus Infection in Patients Undergoing Hemodialysis Acta Medica Iranica Occult hepatitis B Hemodialysis Enzyme-linked immunosorbent assay (ELISA) Nested polymerase chain reaction (Nested-PCR) |
author_facet |
Shahab Mahmoudvand Somayeh Shokri Habibollah Mirzaei Manoochehr Makvandi Ali Teimoori Niloofar Neisi Roya Pirmoradi Ali Ramazani |
author_sort |
Shahab Mahmoudvand |
title |
Study on the Prevalence of Occult Hepatitis B Virus Infection in Patients Undergoing Hemodialysis |
title_short |
Study on the Prevalence of Occult Hepatitis B Virus Infection in Patients Undergoing Hemodialysis |
title_full |
Study on the Prevalence of Occult Hepatitis B Virus Infection in Patients Undergoing Hemodialysis |
title_fullStr |
Study on the Prevalence of Occult Hepatitis B Virus Infection in Patients Undergoing Hemodialysis |
title_full_unstemmed |
Study on the Prevalence of Occult Hepatitis B Virus Infection in Patients Undergoing Hemodialysis |
title_sort |
study on the prevalence of occult hepatitis b virus infection in patients undergoing hemodialysis |
publisher |
Tehran University of Medical Sciences |
series |
Acta Medica Iranica |
issn |
0044-6025 1735-9694 |
publishDate |
2020-07-01 |
description |
Occult hepatitis B (OBI) is a major challenging clinical entity characterized by the absence of hepatitis B surface antigen (HBsAg). The persistence of OBI may progress to fibrosis, cirrhosis, and hepatocellular carcinoma. This study was aimed to investigate the prevalence of OBI among HD patients. In the present cross-sectional study, 89 sera samples of hemodialysis individuals were tested for HBsAg and HBc-IgG by Enzyme-linked Immunosorbent Assay (ELISA). In addition, the HBV DNA has tested in sera and peripheral blood mononuclear cell (PBMC) samples by nested-PCR. Out of 89 patients, 51(57.3%) were males, and 38 (42.7%) females. The ages ranged from 24 to 90 years (with a mean of 57.5±1.37 years). All the sera samples had normal levels of Aspartate Aminotransferase (AST) and Alanine Transaminase (ALT) but had high levels of Creatinine (Cr) (6.9±2.17) and Blood Urea Nitrogen (BUN) (61.83±2.03). 2/89 (2.2%) sera samples were positive for both HBsAg and HBc-IgG test; in addition, HBV DNA was detected in both sera and their PBMC samples. The sera of 15/89 (16.85%) were only positive for the HBc-IgG test, including 10/51 (19.6%) males and 5/38 (13.2%) females (P=0.5). The high 16.85% prevalence OBI has been found among HD patients. To manage OBI infection, screening of HBV DNA should be implemented for HD patients by sensitive molecular means such as nested-PCR and real-time PCR.
|
topic |
Occult hepatitis B Hemodialysis Enzyme-linked immunosorbent assay (ELISA) Nested polymerase chain reaction (Nested-PCR) |
url |
https://acta.tums.ac.ir/index.php/acta/article/view/8206 |
work_keys_str_mv |
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