Intracellular ROS Scavenging and Anti-Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN-γ-Activated RAW264.7 Macrophages
Oroxylum indicum (L.) Kurz has been used as plant-based food and herbal medicine in many Asian countries. The aim of the present study was to examine the antioxidant and anti-inflammatory activities of O. indicum extract (O. indicum) in RAW264.7 cells activated by LPS plus IFN-γ. The phytochemical c...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2020-01-01
|
Series: | Evidence-Based Complementary and Alternative Medicine |
Online Access: | http://dx.doi.org/10.1155/2020/7436920 |
id |
doaj-76452d0648f747e7abb306a5b6c2ddf2 |
---|---|
record_format |
Article |
spelling |
doaj-76452d0648f747e7abb306a5b6c2ddf22020-11-25T02:36:39ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882020-01-01202010.1155/2020/74369207436920Intracellular ROS Scavenging and Anti-Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN-γ-Activated RAW264.7 MacrophagesBenjawan Dunkhunthod0Chutima Talabnin1Mark Murphy2Kanjana Thumanu3Patcharawan Sittisart4Tanaporn Hengpratom5Griangsak Eumkeb6School of Preclinic, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandSchool of Chemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandSchool of Biomolecular Science, Liverpool John Moores University, Liverpool L3 3AF, UKSynchrotron Light Research Institute (Public Organization), Nakhon Ratchasima 30000, ThailandDivision of Environmental Science, Faculty of Liberal Arts and Science, Sisaket Rajabhat University, Sisaket 33000, ThailandSchool of Preclinic, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandSchool of Preclinic, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandOroxylum indicum (L.) Kurz has been used as plant-based food and herbal medicine in many Asian countries. The aim of the present study was to examine the antioxidant and anti-inflammatory activities of O. indicum extract (O. indicum) in RAW264.7 cells activated by LPS plus IFN-γ. The phytochemical compounds in O. indicum were identified by GC-MS and LC-MS/MS. Five flavonoids (luteolin, apigenin, baicalein, oroxylin A, and quercetin) and 27 volatile compounds were found in O. indicum. O. indicum presented antioxidant activities, including reducing ability by FRAP assay and free radical scavenging activity by DPPH assay. Moreover, O. indicum also suppressed LPS plus IFN-γ-activated reactive oxygen species generation in RAW264.7 macrophages. It possessed the potent anti-inflammatory action through suppressing nitric oxide (NO) and IL-6 secretion, possibly due to its ability to scavenge intracellular ROS. The synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectroscopy results showed the alteration of signal intensity and integrated areas relating to lipid and protein of the activated RAW264.7 macrophages compared to unactivated cells. This is the first report of an application of the SR-FTIR technique to evaluate biomolecular changes in activated RAW264.7 cells. Our results indicate that O. indicum may be used as a potential source of nutraceutical for the development of health food supplement or a novel anti-inflammatory herbal medicine.http://dx.doi.org/10.1155/2020/7436920 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Benjawan Dunkhunthod Chutima Talabnin Mark Murphy Kanjana Thumanu Patcharawan Sittisart Tanaporn Hengpratom Griangsak Eumkeb |
spellingShingle |
Benjawan Dunkhunthod Chutima Talabnin Mark Murphy Kanjana Thumanu Patcharawan Sittisart Tanaporn Hengpratom Griangsak Eumkeb Intracellular ROS Scavenging and Anti-Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN-γ-Activated RAW264.7 Macrophages Evidence-Based Complementary and Alternative Medicine |
author_facet |
Benjawan Dunkhunthod Chutima Talabnin Mark Murphy Kanjana Thumanu Patcharawan Sittisart Tanaporn Hengpratom Griangsak Eumkeb |
author_sort |
Benjawan Dunkhunthod |
title |
Intracellular ROS Scavenging and Anti-Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN-γ-Activated RAW264.7 Macrophages |
title_short |
Intracellular ROS Scavenging and Anti-Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN-γ-Activated RAW264.7 Macrophages |
title_full |
Intracellular ROS Scavenging and Anti-Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN-γ-Activated RAW264.7 Macrophages |
title_fullStr |
Intracellular ROS Scavenging and Anti-Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN-γ-Activated RAW264.7 Macrophages |
title_full_unstemmed |
Intracellular ROS Scavenging and Anti-Inflammatory Activities of Oroxylum indicum Kurz (L.) Extract in LPS plus IFN-γ-Activated RAW264.7 Macrophages |
title_sort |
intracellular ros scavenging and anti-inflammatory activities of oroxylum indicum kurz (l.) extract in lps plus ifn-γ-activated raw264.7 macrophages |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2020-01-01 |
description |
Oroxylum indicum (L.) Kurz has been used as plant-based food and herbal medicine in many Asian countries. The aim of the present study was to examine the antioxidant and anti-inflammatory activities of O. indicum extract (O. indicum) in RAW264.7 cells activated by LPS plus IFN-γ. The phytochemical compounds in O. indicum were identified by GC-MS and LC-MS/MS. Five flavonoids (luteolin, apigenin, baicalein, oroxylin A, and quercetin) and 27 volatile compounds were found in O. indicum. O. indicum presented antioxidant activities, including reducing ability by FRAP assay and free radical scavenging activity by DPPH assay. Moreover, O. indicum also suppressed LPS plus IFN-γ-activated reactive oxygen species generation in RAW264.7 macrophages. It possessed the potent anti-inflammatory action through suppressing nitric oxide (NO) and IL-6 secretion, possibly due to its ability to scavenge intracellular ROS. The synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectroscopy results showed the alteration of signal intensity and integrated areas relating to lipid and protein of the activated RAW264.7 macrophages compared to unactivated cells. This is the first report of an application of the SR-FTIR technique to evaluate biomolecular changes in activated RAW264.7 cells. Our results indicate that O. indicum may be used as a potential source of nutraceutical for the development of health food supplement or a novel anti-inflammatory herbal medicine. |
url |
http://dx.doi.org/10.1155/2020/7436920 |
work_keys_str_mv |
AT benjawandunkhunthod intracellularrosscavengingandantiinflammatoryactivitiesoforoxylumindicumkurzlextractinlpsplusifngactivatedraw2647macrophages AT chutimatalabnin intracellularrosscavengingandantiinflammatoryactivitiesoforoxylumindicumkurzlextractinlpsplusifngactivatedraw2647macrophages AT markmurphy intracellularrosscavengingandantiinflammatoryactivitiesoforoxylumindicumkurzlextractinlpsplusifngactivatedraw2647macrophages AT kanjanathumanu intracellularrosscavengingandantiinflammatoryactivitiesoforoxylumindicumkurzlextractinlpsplusifngactivatedraw2647macrophages AT patcharawansittisart intracellularrosscavengingandantiinflammatoryactivitiesoforoxylumindicumkurzlextractinlpsplusifngactivatedraw2647macrophages AT tanapornhengpratom intracellularrosscavengingandantiinflammatoryactivitiesoforoxylumindicumkurzlextractinlpsplusifngactivatedraw2647macrophages AT griangsakeumkeb intracellularrosscavengingandantiinflammatoryactivitiesoforoxylumindicumkurzlextractinlpsplusifngactivatedraw2647macrophages |
_version_ |
1715436690712035328 |