Interaction of Natural Compounds in Licorice and Turmeric with HIV-NCp7 Zinc Finger Domain: Potential Relevance to the Mechanism of Antiviral Activity
Nucleocapsid proteins (NCp) are zinc finger (ZF) proteins, and they play a central role in HIV virus replication, mainly by interacting with nucleic acids. Therefore, they are potential targets for anti-HIV therapy. Natural products have been shown to be able to inhibit HIV, such as turmeric and lic...
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doaj-766149150b7a4c1fbf116f0fdc83887b2021-06-30T23:54:02ZengMDPI AGMolecules1420-30492021-06-01263563356310.3390/molecules26123563Interaction of Natural Compounds in Licorice and Turmeric with HIV-NCp7 Zinc Finger Domain: Potential Relevance to the Mechanism of Antiviral ActivityRunjing Wang0Yinyu Wei1Meiqin Wang2Pan Yan3Hongliang Jiang4Zhifeng Du5Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, ChinaTongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, ChinaTongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, ChinaTongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, ChinaTongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, ChinaTongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, ChinaNucleocapsid proteins (NCp) are zinc finger (ZF) proteins, and they play a central role in HIV virus replication, mainly by interacting with nucleic acids. Therefore, they are potential targets for anti-HIV therapy. Natural products have been shown to be able to inhibit HIV, such as turmeric and licorice, which is widely used in traditional Chinese medicine. Liquiritin (LQ), isoliquiritin (ILQ), glycyrrhizic acid (GL), glycyrrhetinic acid (GA) and curcumin (CUR), which were the major active components, were herein chosen to study their interactions with HIV-NCp7 C-terminal zinc finger, aiming to find the potential active compounds and reveal the mechanism involved. The stacking interaction between NCp7 tryptophan and natural compounds was evaluated by fluorescence. To elucidate the binding mode, mass spectrometry was used to characterize the reaction mixture between zinc finger proteins and active compounds. Subsequently, circular dichroism (CD) spectroscopy and molecular docking were used to validate and reveal the binding mode from a structural perspective. The results showed that ILQ has the strongest binding ability among the tested compounds, followed by curcumin, and the interaction between ILQ and the NCp7 zinc finger peptide was mediated by a noncovalent interaction. This study provided a scientific basis for the antiviral activity of turmeric and licorice.https://www.mdpi.com/1420-3049/26/12/3563zinc fingersfluorescencemass spectrometryanti-HIVnatural product |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Runjing Wang Yinyu Wei Meiqin Wang Pan Yan Hongliang Jiang Zhifeng Du |
spellingShingle |
Runjing Wang Yinyu Wei Meiqin Wang Pan Yan Hongliang Jiang Zhifeng Du Interaction of Natural Compounds in Licorice and Turmeric with HIV-NCp7 Zinc Finger Domain: Potential Relevance to the Mechanism of Antiviral Activity Molecules zinc fingers fluorescence mass spectrometry anti-HIV natural product |
author_facet |
Runjing Wang Yinyu Wei Meiqin Wang Pan Yan Hongliang Jiang Zhifeng Du |
author_sort |
Runjing Wang |
title |
Interaction of Natural Compounds in Licorice and Turmeric with HIV-NCp7 Zinc Finger Domain: Potential Relevance to the Mechanism of Antiviral Activity |
title_short |
Interaction of Natural Compounds in Licorice and Turmeric with HIV-NCp7 Zinc Finger Domain: Potential Relevance to the Mechanism of Antiviral Activity |
title_full |
Interaction of Natural Compounds in Licorice and Turmeric with HIV-NCp7 Zinc Finger Domain: Potential Relevance to the Mechanism of Antiviral Activity |
title_fullStr |
Interaction of Natural Compounds in Licorice and Turmeric with HIV-NCp7 Zinc Finger Domain: Potential Relevance to the Mechanism of Antiviral Activity |
title_full_unstemmed |
Interaction of Natural Compounds in Licorice and Turmeric with HIV-NCp7 Zinc Finger Domain: Potential Relevance to the Mechanism of Antiviral Activity |
title_sort |
interaction of natural compounds in licorice and turmeric with hiv-ncp7 zinc finger domain: potential relevance to the mechanism of antiviral activity |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2021-06-01 |
description |
Nucleocapsid proteins (NCp) are zinc finger (ZF) proteins, and they play a central role in HIV virus replication, mainly by interacting with nucleic acids. Therefore, they are potential targets for anti-HIV therapy. Natural products have been shown to be able to inhibit HIV, such as turmeric and licorice, which is widely used in traditional Chinese medicine. Liquiritin (LQ), isoliquiritin (ILQ), glycyrrhizic acid (GL), glycyrrhetinic acid (GA) and curcumin (CUR), which were the major active components, were herein chosen to study their interactions with HIV-NCp7 C-terminal zinc finger, aiming to find the potential active compounds and reveal the mechanism involved. The stacking interaction between NCp7 tryptophan and natural compounds was evaluated by fluorescence. To elucidate the binding mode, mass spectrometry was used to characterize the reaction mixture between zinc finger proteins and active compounds. Subsequently, circular dichroism (CD) spectroscopy and molecular docking were used to validate and reveal the binding mode from a structural perspective. The results showed that ILQ has the strongest binding ability among the tested compounds, followed by curcumin, and the interaction between ILQ and the NCp7 zinc finger peptide was mediated by a noncovalent interaction. This study provided a scientific basis for the antiviral activity of turmeric and licorice. |
topic |
zinc fingers fluorescence mass spectrometry anti-HIV natural product |
url |
https://www.mdpi.com/1420-3049/26/12/3563 |
work_keys_str_mv |
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