Detection of SHV and TEM-type Extended spectrum β-lactamase in bacterial isolates recovered from clinical samples of patients attending military hospitals
Background Multi-drug resistant bacterial strains have been increasingly implicated in clinical infections worldwide and beta-lactamase production is one of the commonest mechanisms of resistance in these strains. This study investigated the prevalence of extended spectrum â-lactamase (ESBL)-produci...
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doaj-76753ee39ae243d2bb794e6a8226e77a2021-08-15T06:22:11ZengFaculty of Medicine Trisakti UniversityUniversa Medicina1907-30622407-22302019-12-0138310.18051/UnivMed.2019.v38.186-193389Detection of SHV and TEM-type Extended spectrum β-lactamase in bacterial isolates recovered from clinical samples of patients attending military hospitalsHelen Oroboghae Ogefere0Samuel E. Iriah1Ephraim Ehidiamen Ibadin2Dept of Medical Laboratory Science, Faculty of Basic Medical Sciences, University of Benin, Benin City, NigeriaPathology Department, Medical Services and Hospital, 81 Division, Dodan Barracks, Ikoyi, Lagos StateMedical Microbiology Unit, Medical Laboratory Services, University of Benin Teaching Hospital, Benin City, NigeriaBackground Multi-drug resistant bacterial strains have been increasingly implicated in clinical infections worldwide and beta-lactamase production is one of the commonest mechanisms of resistance in these strains. This study investigated the prevalence of extended spectrum â-lactamase (ESBL)-producing isolates and determined the temoneira (TEM) and sulfhydryl variable (SHV) types implicated in two military hospitals in South-South Nigeria. Methods Three-hundred and eighty (380) consecutive non-duplicate bacterial isolates (Gram negative bacilli) recovered from clinical samples were identified following standard techniques. Antimicrobial susceptibility tests were performed for each isolate following the Clinical Laboratory Standards Institute guidelines. Bacterial isolates recovered which comprised Escherichia coli, Klebsiella spp, Proteus spp and Pseudomonas aeruginosa were screened for ESBL using a phenotypic method (double disc synergy test). All positive isolates were screened for TEM and SHV genes by PCR method. Results Sixty-five isolates (17.1%) were ESBL producing using phenotypic method, E. coli showed the highest ESBL prevalence (24.3%). One isolate was SHV positive (1.5%), 8 (12.3%) were TEM positive while 3 (4.6%) isolates harbored both SHV and TEM genes. Fluoroquinolone - ofloxacin showed marked activity against ESBL-producing isolates (90.8%) while the least active were ceftriaxone (9.2%), ceftazidime (3.1%) and ampicillin (1.5%). Conclusion This study demonstrated that 17.1% of Gram-negative bacilli were ESBL producers. Screening of clinical isolates for ESBL should be implemented. The findings of this study suggest the need for caution in the use of antimicrobial agents in order to curb the incidence of antimicrobial resistance.https://univmed.org/ejurnal/index.php/medicina/article/view/889Extended spectrum β-lactamaseresistanceGram negative bacilliantimicrobial |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Helen Oroboghae Ogefere Samuel E. Iriah Ephraim Ehidiamen Ibadin |
spellingShingle |
Helen Oroboghae Ogefere Samuel E. Iriah Ephraim Ehidiamen Ibadin Detection of SHV and TEM-type Extended spectrum β-lactamase in bacterial isolates recovered from clinical samples of patients attending military hospitals Universa Medicina Extended spectrum β-lactamase resistance Gram negative bacilli antimicrobial |
author_facet |
Helen Oroboghae Ogefere Samuel E. Iriah Ephraim Ehidiamen Ibadin |
author_sort |
Helen Oroboghae Ogefere |
title |
Detection of SHV and TEM-type Extended spectrum β-lactamase in bacterial isolates recovered from clinical samples of patients attending military hospitals |
title_short |
Detection of SHV and TEM-type Extended spectrum β-lactamase in bacterial isolates recovered from clinical samples of patients attending military hospitals |
title_full |
Detection of SHV and TEM-type Extended spectrum β-lactamase in bacterial isolates recovered from clinical samples of patients attending military hospitals |
title_fullStr |
Detection of SHV and TEM-type Extended spectrum β-lactamase in bacterial isolates recovered from clinical samples of patients attending military hospitals |
title_full_unstemmed |
Detection of SHV and TEM-type Extended spectrum β-lactamase in bacterial isolates recovered from clinical samples of patients attending military hospitals |
title_sort |
detection of shv and tem-type extended spectrum β-lactamase in bacterial isolates recovered from clinical samples of patients attending military hospitals |
publisher |
Faculty of Medicine Trisakti University |
series |
Universa Medicina |
issn |
1907-3062 2407-2230 |
publishDate |
2019-12-01 |
description |
Background
Multi-drug resistant bacterial strains have been increasingly implicated in clinical infections worldwide and beta-lactamase production is one of the commonest mechanisms of resistance in these strains. This study investigated the prevalence of extended spectrum â-lactamase (ESBL)-producing isolates and determined the temoneira (TEM) and sulfhydryl variable (SHV) types implicated in two military hospitals in South-South Nigeria.
Methods
Three-hundred and eighty (380) consecutive non-duplicate bacterial isolates (Gram negative bacilli) recovered from clinical samples were identified following standard techniques. Antimicrobial susceptibility tests were performed for each isolate following the Clinical Laboratory Standards Institute guidelines. Bacterial isolates recovered which comprised Escherichia coli, Klebsiella spp, Proteus spp and Pseudomonas aeruginosa were screened for ESBL using a phenotypic method (double disc synergy test). All positive isolates were screened for TEM and SHV genes by PCR method.
Results
Sixty-five isolates (17.1%) were ESBL producing using phenotypic method, E. coli showed the highest ESBL prevalence (24.3%). One isolate was SHV positive (1.5%), 8 (12.3%) were TEM positive while 3 (4.6%) isolates harbored both SHV and TEM genes. Fluoroquinolone - ofloxacin showed marked activity against ESBL-producing isolates (90.8%) while the least active were ceftriaxone (9.2%), ceftazidime (3.1%) and ampicillin (1.5%).
Conclusion
This study demonstrated that 17.1% of Gram-negative bacilli were ESBL producers. Screening of clinical isolates for ESBL should be implemented. The findings of this study suggest the need for caution in the use of antimicrobial agents in order to curb the incidence of antimicrobial resistance. |
topic |
Extended spectrum β-lactamase resistance Gram negative bacilli antimicrobial |
url |
https://univmed.org/ejurnal/index.php/medicina/article/view/889 |
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