Microbial transformation of ginsenoside Rb1, Re and Rg1 and its contribution to the improved anti-inflammatory activity of ginseng
Abstract Microbial transformation of ginsenosides to increase its pharmaceutical effect is gaining increasing attention in recent years. In this study, Cellulosimicrobium sp. TH-20, which was isolated from soil samples on which ginseng grown, exhibited effective ginsenoside-transforming activity. Af...
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doaj-768a3625ac5648ea82b43a6952266b172020-12-08T01:31:27ZengNature Publishing GroupScientific Reports2045-23222017-03-017111010.1038/s41598-017-00262-0Microbial transformation of ginsenoside Rb1, Re and Rg1 and its contribution to the improved anti-inflammatory activity of ginsengShanshan Yu0Xiaoli Zhou1Fan Li2Chunchun Xu3Fei Zheng4Jing Li5Huanxi Zhao6Yulin Dai7Shuying Liu8Yan Feng9State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong UniversityCollege of Basic Medical Sciences, Jilin UniversitySchool of Life Sciences, Northeast Normal UniversityJilin Ginseng Academy, Changchun University of Chinese MedicineJilin Ginseng Academy, Changchun University of Chinese MedicineJilin Ginseng Academy, Changchun University of Chinese MedicineJilin Ginseng Academy, Changchun University of Chinese MedicineJilin Ginseng Academy, Changchun University of Chinese MedicineJilin Ginseng Academy, Changchun University of Chinese MedicineState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong UniversityAbstract Microbial transformation of ginsenosides to increase its pharmaceutical effect is gaining increasing attention in recent years. In this study, Cellulosimicrobium sp. TH-20, which was isolated from soil samples on which ginseng grown, exhibited effective ginsenoside-transforming activity. After protopanaxadiol (PPD)-type ginsenoside (Rb1) and protopanaxatriol (PPT)-type ginsenosides (Re and Rg1) were fed to C. sp. TH20, a total of 12 metabolites, including 6 new intermediate metabolites, were identified. Stepwise deglycosylation and dehydrogenation on the feeding precursors have been observed. The final products were confirmed to be rare ginsenosides Rd, GypXVII, Rg2 and PPT after 96 h transformation with 38–96% yields. The four products showed improved anti-inflammatory activities by using lipopolysaccharide (LPS)-induced murine RAW 264.7 macrophages and the xylene-induced acute inflammatory model of mouse ear edema. The results indicated that they could dramatically attenuate the production of TNF-α more effectively than the precursors. Our study would provide an example of a unique and powerful microbial cell factory for efficiently converting both PPD-type and PPT-type ginsenosides to rare natural products, which extends the drug candidates as novel anti-inflammatory remedies.https://doi.org/10.1038/s41598-017-00262-0 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shanshan Yu Xiaoli Zhou Fan Li Chunchun Xu Fei Zheng Jing Li Huanxi Zhao Yulin Dai Shuying Liu Yan Feng |
spellingShingle |
Shanshan Yu Xiaoli Zhou Fan Li Chunchun Xu Fei Zheng Jing Li Huanxi Zhao Yulin Dai Shuying Liu Yan Feng Microbial transformation of ginsenoside Rb1, Re and Rg1 and its contribution to the improved anti-inflammatory activity of ginseng Scientific Reports |
author_facet |
Shanshan Yu Xiaoli Zhou Fan Li Chunchun Xu Fei Zheng Jing Li Huanxi Zhao Yulin Dai Shuying Liu Yan Feng |
author_sort |
Shanshan Yu |
title |
Microbial transformation of ginsenoside Rb1, Re and Rg1 and its contribution to the improved anti-inflammatory activity of ginseng |
title_short |
Microbial transformation of ginsenoside Rb1, Re and Rg1 and its contribution to the improved anti-inflammatory activity of ginseng |
title_full |
Microbial transformation of ginsenoside Rb1, Re and Rg1 and its contribution to the improved anti-inflammatory activity of ginseng |
title_fullStr |
Microbial transformation of ginsenoside Rb1, Re and Rg1 and its contribution to the improved anti-inflammatory activity of ginseng |
title_full_unstemmed |
Microbial transformation of ginsenoside Rb1, Re and Rg1 and its contribution to the improved anti-inflammatory activity of ginseng |
title_sort |
microbial transformation of ginsenoside rb1, re and rg1 and its contribution to the improved anti-inflammatory activity of ginseng |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-03-01 |
description |
Abstract Microbial transformation of ginsenosides to increase its pharmaceutical effect is gaining increasing attention in recent years. In this study, Cellulosimicrobium sp. TH-20, which was isolated from soil samples on which ginseng grown, exhibited effective ginsenoside-transforming activity. After protopanaxadiol (PPD)-type ginsenoside (Rb1) and protopanaxatriol (PPT)-type ginsenosides (Re and Rg1) were fed to C. sp. TH20, a total of 12 metabolites, including 6 new intermediate metabolites, were identified. Stepwise deglycosylation and dehydrogenation on the feeding precursors have been observed. The final products were confirmed to be rare ginsenosides Rd, GypXVII, Rg2 and PPT after 96 h transformation with 38–96% yields. The four products showed improved anti-inflammatory activities by using lipopolysaccharide (LPS)-induced murine RAW 264.7 macrophages and the xylene-induced acute inflammatory model of mouse ear edema. The results indicated that they could dramatically attenuate the production of TNF-α more effectively than the precursors. Our study would provide an example of a unique and powerful microbial cell factory for efficiently converting both PPD-type and PPT-type ginsenosides to rare natural products, which extends the drug candidates as novel anti-inflammatory remedies. |
url |
https://doi.org/10.1038/s41598-017-00262-0 |
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