E2/ER change and its relation with FasL expression in breast cancer patients before and after menopause

• Main Authors: Yu Liu, Bing-Yu Gao, Li-Ping Xia, Yu-You Wu, Yu Wang, Chao-Qun Wang
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2016-10-01
• Series: Journal of Hainan Medical University
• Subjects: Breast cancer; Before menopause; After menopause; E2; ER; FasL
• Online Access: http://www.hnykdxxb.com/PDF/201620/25.pdf

Objective: To explore the E2/ER changes both in the peripheral blood and tumors, as well as its effect on factor-related apoptosis ligand (FasL) gene expression of breast cancer patients before and after menopause. Methods: We chose premenopausal (n=21) and postmenopausal (n=19) breast cancer patients in this study to do a comparative analyze. Peripheral blood was extracted to detect the E2 and ER concentration by ELISA kit. At the same time, pathological slices of tumor and healthy tissue besides the tumor were both prepared to detect the E2, ER and FasL expression with immunofluorescence. Results: Postmenopausal patients had significantly lower peripheral levels of E2 and ER than premenopausal patients, while tumor levels of E2 and ER had no significant difference between groups. Further more, ER positive expression rate of patients before and after menopause was respectively 52.4% and 57.9% with out statistical differences between groups; and FasL gene expression had linear positive correlation with tumor E2 and ER expression both in patients with ER positive expression before and after menopause. Conclusion: Our research results show that E2 and ER level tumor tissues could keep high level independent of environment which contributes the progress of breast caner; and high dose E2 and ER level of the tumor tissues which up regulated the expression of FasL maybe an important factor for immune escape.