Development of method quantitative content of dihydroquercetin. Report 1

• Main Authors: Олександр Юрійович Владимиров, Вікторія Акоповна Георгіянц, Ганна Олександрівна Бур’ян, Шарк Амжад Ібрагім Абу, Лідія Олександрівна Петрушова, Тетяна Вікторівна Алексєєва, Олександр Анатолійович Здорик, Василь Васильович Гриненко, Ірина Анатоліївна Данилова
• Format: Article
• Language: English
• Published: PC Technology Center 2016-01-01
• Series: ScienceRise
• Subjects: photocolorimetric method; quantitative determination; validation characteristics; the State Pharmacopoeia of Ukraine; dihydroquercetin
• Online Access: http://journals.uran.ua/sciencerise/article/view/59243
• ISSN: 2313-6286; 2313-8416

<p>Today is markedly increasing scientific interest in the study of flavonoids in plant objects due to their high biological activity. In this regard, the urgent task of analytical chemistry is in developing available analytical techniques of determination for flavonoids in plant objects.</p><p><strong>Aim.</strong> The aim was to develop specific techniques of quantitative determination for dihydroquercetin and determination of its validation characteristics.</p><p><strong>Methods.</strong> The technique for photocolorimetric quantification of DQW, which was based on the specific reaction of cyanidine chloride formation when added zinc powder to dihydroquercetin solution in an acidic medium has been elaborated.</p><p><strong>Results.</strong> Photocolorimetric technique of determining flavonoids recalculating on DQW has been developed, its basic validation characteristics have been determined. The obtained metrological characteristics of photocolorimetric technique for determining DQW did not exceed admissibility criteria in accordance with the requirements of the State Pharmacopoeia of Ukraine.</p><p><strong>Conclusions.</strong> By the results of statistical analysis of experimental data, it has been stated that the developed technique can be used for quantification of DQW. Metrological data obtained indicate that the method reproduced in conditions of two different laboratories with confidence probability 95 % unit value deviation was 101,85±2,54 %</p>