Experimental Investigation of Ki67, POU5F1, and ZBTB16 Expression in the Pig and Mouse Testicular Cells using Immunocytochemistry and RT-PCR
Introduction: Spermatogonial Stem Cells (SSC) are the originators and beginning points of the spermatogenesis process. Moreover, they are considered the only stem cells in the body that could transfer genetic information to the next generation through gametogenesis. This study aimed to investigate t...
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Ilam University of Medical Sciences
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doaj-76b4a486dde64d8fb7c4e3247358bbae2021-02-23T09:21:29Zfas Ilam University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām1563-47282588-31352020-12-01285110Experimental Investigation of Ki67, POU5F1, and ZBTB16 Expression in the Pig and Mouse Testicular Cells using Immunocytochemistry and RT-PCRHossein Azizi0Amirreza Niazi Tabar1Atiyeh Mohammadi2 Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran Introduction: Spermatogonial Stem Cells (SSC) are the originators and beginning points of the spermatogenesis process. Moreover, they are considered the only stem cells in the body that could transfer genetic information to the next generation through gametogenesis. This study aimed to investigate the potency and power of SSC under in vitro and in vivo conditions. Materials & Methods: Enzymatic digestion technique was utilized to extract the spermatogonial cells of the pig and mousechr('39')s testis. They were then cultured in an environment containing FGF, EGF, GDNF, and a feeder layer of STO. For immunocytochemistry and RT-PCR analysis, Ki67, POU5F1, and ZBTB16 markers were used to evaluate the resulted colonies. Ethics code: Ir.ausmt.rec.1398.03.07 Findings: The nature of the SSC resulted after separation and culture was proved through measures, such as cluster growth of the colonies in the culture medium, Ki67 marker expression in the immunocytochemistry review which showed the duplication ability, and the morphological criteria observed by an electron microscope. Moreover, the comparative expression of POU5F1 and ZBTB16 markers in the embryonic stem cells, SSC, and Sertoli cells within the seminiferous tubules of the mouse was analyzed by RT-PCR. Discussions & Conclusions: This experimental study investigated the expression of Ki67, POU5F1, and ZBTB16 in the seminiferous tubules and special cytological features of SCC. The findings are beneficial for future advanced studies in reproductive biology fields.http://sjimu.medilam.ac.ir/article-1-6238-en.htmlcytologymouse embryonic stem cellspluripotent stem cellspou5f1 proteinzbtb16 |
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DOAJ |
language |
fas |
format |
Article |
sources |
DOAJ |
author |
Hossein Azizi Amirreza Niazi Tabar Atiyeh Mohammadi |
spellingShingle |
Hossein Azizi Amirreza Niazi Tabar Atiyeh Mohammadi Experimental Investigation of Ki67, POU5F1, and ZBTB16 Expression in the Pig and Mouse Testicular Cells using Immunocytochemistry and RT-PCR Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām cytology mouse embryonic stem cells pluripotent stem cells pou5f1 protein zbtb16 |
author_facet |
Hossein Azizi Amirreza Niazi Tabar Atiyeh Mohammadi |
author_sort |
Hossein Azizi |
title |
Experimental Investigation of Ki67, POU5F1, and ZBTB16
Expression in the Pig and Mouse Testicular Cells using Immunocytochemistry and RT-PCR |
title_short |
Experimental Investigation of Ki67, POU5F1, and ZBTB16
Expression in the Pig and Mouse Testicular Cells using Immunocytochemistry and RT-PCR |
title_full |
Experimental Investigation of Ki67, POU5F1, and ZBTB16
Expression in the Pig and Mouse Testicular Cells using Immunocytochemistry and RT-PCR |
title_fullStr |
Experimental Investigation of Ki67, POU5F1, and ZBTB16
Expression in the Pig and Mouse Testicular Cells using Immunocytochemistry and RT-PCR |
title_full_unstemmed |
Experimental Investigation of Ki67, POU5F1, and ZBTB16
Expression in the Pig and Mouse Testicular Cells using Immunocytochemistry and RT-PCR |
title_sort |
experimental investigation of ki67, pou5f1, and zbtb16
expression in the pig and mouse testicular cells using immunocytochemistry and rt-pcr |
publisher |
Ilam University of Medical Sciences |
series |
Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
issn |
1563-4728 2588-3135 |
publishDate |
2020-12-01 |
description |
Introduction: Spermatogonial Stem Cells (SSC) are the originators and beginning points of the spermatogenesis process. Moreover, they are considered the only stem cells in the body that could transfer genetic information to the next generation through gametogenesis. This study aimed to investigate the potency and power of SSC under in vitro and in vivo conditions.
Materials & Methods: Enzymatic digestion technique was utilized to extract the spermatogonial cells of the pig and mousechr('39')s testis. They were then cultured in an environment containing FGF, EGF, GDNF, and a feeder layer of STO. For immunocytochemistry and RT-PCR analysis, Ki67, POU5F1, and ZBTB16 markers were used to evaluate the resulted colonies. Ethics code: Ir.ausmt.rec.1398.03.07
Findings: The nature of the SSC resulted after separation and culture was proved through measures, such as cluster growth of the colonies in the culture medium, Ki67 marker expression in the immunocytochemistry review which showed the duplication ability, and the morphological criteria observed by an electron microscope. Moreover, the comparative expression of POU5F1 and ZBTB16 markers in the embryonic stem cells, SSC, and Sertoli cells within the seminiferous tubules of the mouse was analyzed by RT-PCR.
Discussions & Conclusions: This experimental study investigated the expression of Ki67, POU5F1, and ZBTB16 in the seminiferous tubules and special cytological features of SCC. The findings are beneficial for future advanced studies in reproductive biology fields. |
topic |
cytology mouse embryonic stem cells pluripotent stem cells pou5f1 protein zbtb16 |
url |
http://sjimu.medilam.ac.ir/article-1-6238-en.html |
work_keys_str_mv |
AT hosseinazizi experimentalinvestigationofki67pou5f1andzbtb16expressioninthepigandmousetesticularcellsusingimmunocytochemistryandrtpcr AT amirrezaniazitabar experimentalinvestigationofki67pou5f1andzbtb16expressioninthepigandmousetesticularcellsusingimmunocytochemistryandrtpcr AT atiyehmohammadi experimentalinvestigationofki67pou5f1andzbtb16expressioninthepigandmousetesticularcellsusingimmunocytochemistryandrtpcr |
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