Evaluation of the Genotoxic and Oxidative Damage Potential of Silver Nanoparticles in Human NCM460 and HCT116 Cells
Nano Ag has excellent antibacterial properties and is widely used in various antibacterial materials, such as antibacterial medicine and medical devices, food packaging materials and antibacterial textiles. Despite the many benefits of nano-Ag, more and more research indicates that it may have poten...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-02-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/21/5/1618 |
| id |
doaj-76d43df1605c4df7aeb06b64d975abce |
|---|---|
| record_format |
Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Mingxi Jia Wenjing Zhang Taojin He Meng Shu Jing Deng Jianhui Wang Wen Li Jie Bai Qinlu Lin Feijun Luo Wenhua Zhou Xiaoxi Zeng |
| spellingShingle |
Mingxi Jia Wenjing Zhang Taojin He Meng Shu Jing Deng Jianhui Wang Wen Li Jie Bai Qinlu Lin Feijun Luo Wenhua Zhou Xiaoxi Zeng Evaluation of the Genotoxic and Oxidative Damage Potential of Silver Nanoparticles in Human NCM460 and HCT116 Cells International Journal of Molecular Sciences nano-ag colon cells biological toxicity oxidative damage |
| author_facet |
Mingxi Jia Wenjing Zhang Taojin He Meng Shu Jing Deng Jianhui Wang Wen Li Jie Bai Qinlu Lin Feijun Luo Wenhua Zhou Xiaoxi Zeng |
| author_sort |
Mingxi Jia |
| title |
Evaluation of the Genotoxic and Oxidative Damage Potential of Silver Nanoparticles in Human NCM460 and HCT116 Cells |
| title_short |
Evaluation of the Genotoxic and Oxidative Damage Potential of Silver Nanoparticles in Human NCM460 and HCT116 Cells |
| title_full |
Evaluation of the Genotoxic and Oxidative Damage Potential of Silver Nanoparticles in Human NCM460 and HCT116 Cells |
| title_fullStr |
Evaluation of the Genotoxic and Oxidative Damage Potential of Silver Nanoparticles in Human NCM460 and HCT116 Cells |
| title_full_unstemmed |
Evaluation of the Genotoxic and Oxidative Damage Potential of Silver Nanoparticles in Human NCM460 and HCT116 Cells |
| title_sort |
evaluation of the genotoxic and oxidative damage potential of silver nanoparticles in human ncm460 and hct116 cells |
| publisher |
MDPI AG |
| series |
International Journal of Molecular Sciences |
| issn |
1422-0067 |
| publishDate |
2020-02-01 |
| description |
Nano Ag has excellent antibacterial properties and is widely used in various antibacterial materials, such as antibacterial medicine and medical devices, food packaging materials and antibacterial textiles. Despite the many benefits of nano-Ag, more and more research indicates that it may have potential biotoxic effects. Studies have shown that people who ingest nanoparticles by mouth have the highest uptake in the intestinal tract, and that the colon area is the most vulnerable to damage and causes the disease. In this study, we examined the toxic effects of different concentrations of Ag-NPs on normal human colon cells (NCM460) and human colon cancer cells (HCT116). As the concentration of nanoparticles increased, the activity of the two colon cells decreased and intracellular reactive oxygen species (ROS) increased. RT-qPCR and Western-blot analyses showed that Ag NPs can promote the increase in P38 protein phosphorylation levels in two colon cells and promote the expression of P53 and Bax. The analysis also showed that Ag NPs can promote the down-regulation of Bcl-2, leading to an increased Bax/Bcl-2 ratio and activation of P21, further accelerating cell death. This study showed that a low concentration of nano Ag has no obvious toxic effect on colon cells, while nano Ag with concentrations higher than 15 μg/mL will cause oxidative damage to colon cells. |
| topic |
nano-ag colon cells biological toxicity oxidative damage |
| url |
https://www.mdpi.com/1422-0067/21/5/1618 |
| work_keys_str_mv |
AT mingxijia evaluationofthegenotoxicandoxidativedamagepotentialofsilvernanoparticlesinhumanncm460andhct116cells AT wenjingzhang evaluationofthegenotoxicandoxidativedamagepotentialofsilvernanoparticlesinhumanncm460andhct116cells AT taojinhe evaluationofthegenotoxicandoxidativedamagepotentialofsilvernanoparticlesinhumanncm460andhct116cells AT mengshu evaluationofthegenotoxicandoxidativedamagepotentialofsilvernanoparticlesinhumanncm460andhct116cells AT jingdeng evaluationofthegenotoxicandoxidativedamagepotentialofsilvernanoparticlesinhumanncm460andhct116cells AT jianhuiwang evaluationofthegenotoxicandoxidativedamagepotentialofsilvernanoparticlesinhumanncm460andhct116cells AT wenli evaluationofthegenotoxicandoxidativedamagepotentialofsilvernanoparticlesinhumanncm460andhct116cells AT jiebai evaluationofthegenotoxicandoxidativedamagepotentialofsilvernanoparticlesinhumanncm460andhct116cells AT qinlulin evaluationofthegenotoxicandoxidativedamagepotentialofsilvernanoparticlesinhumanncm460andhct116cells AT feijunluo evaluationofthegenotoxicandoxidativedamagepotentialofsilvernanoparticlesinhumanncm460andhct116cells AT wenhuazhou evaluationofthegenotoxicandoxidativedamagepotentialofsilvernanoparticlesinhumanncm460andhct116cells AT xiaoxizeng evaluationofthegenotoxicandoxidativedamagepotentialofsilvernanoparticlesinhumanncm460andhct116cells |
| _version_ |
1725867952770646016 |
| spelling |
doaj-76d43df1605c4df7aeb06b64d975abce2020-11-24T21:54:16ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-02-01215161810.3390/ijms21051618ijms21051618Evaluation of the Genotoxic and Oxidative Damage Potential of Silver Nanoparticles in Human NCM460 and HCT116 CellsMingxi Jia0Wenjing Zhang1Taojin He2Meng Shu3Jing Deng4Jianhui Wang5Wen Li6Jie Bai7Qinlu Lin8Feijun Luo9Wenhua Zhou10Xiaoxi Zeng11Hunan Key Laboratory of Processed Food for Special Medical Purpose, Hunan Key Laboratory of Grain-Oil Deep Process and Quality Control, College of Food Science and Engineering, National Engineering Laboratory for Deep Process of Rice and Byproducts, Central South University of Forestry and Technology, Changsha 410004, ChinaHunan Key Laboratory of Processed Food for Special Medical Purpose, Hunan Key Laboratory of Grain-Oil Deep Process and Quality Control, College of Food Science and Engineering, National Engineering Laboratory for Deep Process of Rice and Byproducts, Central South University of Forestry and Technology, Changsha 410004, ChinaHunan Key Laboratory of Processed Food for Special Medical Purpose, Hunan Key Laboratory of Grain-Oil Deep Process and Quality Control, College of Food Science and Engineering, National Engineering Laboratory for Deep Process of Rice and Byproducts, Central South University of Forestry and Technology, Changsha 410004, ChinaHunan Key Laboratory of Processed Food for Special Medical Purpose, Hunan Key Laboratory of Grain-Oil Deep Process and Quality Control, College of Food Science and Engineering, National Engineering Laboratory for Deep Process of Rice and Byproducts, Central South University of Forestry and Technology, Changsha 410004, ChinaHunan Key Laboratory of Processed Food for Special Medical Purpose, Hunan Key Laboratory of Grain-Oil Deep Process and Quality Control, College of Food Science and Engineering, National Engineering Laboratory for Deep Process of Rice and Byproducts, Central South University of Forestry and Technology, Changsha 410004, ChinaSchool of Chemistry and Food Engineering, Changsha University of Science and Technology, Changsha 410114, ChinaHunan Key Laboratory of Processed Food for Special Medical Purpose, Hunan Key Laboratory of Grain-Oil Deep Process and Quality Control, College of Food Science and Engineering, National Engineering Laboratory for Deep Process of Rice and Byproducts, Central South University of Forestry and Technology, Changsha 410004, ChinaHunan Key Laboratory of Processed Food for Special Medical Purpose, Hunan Key Laboratory of Grain-Oil Deep Process and Quality Control, College of Food Science and Engineering, National Engineering Laboratory for Deep Process of Rice and Byproducts, Central South University of Forestry and Technology, Changsha 410004, ChinaHunan Key Laboratory of Processed Food for Special Medical Purpose, Hunan Key Laboratory of Grain-Oil Deep Process and Quality Control, College of Food Science and Engineering, National Engineering Laboratory for Deep Process of Rice and Byproducts, Central South University of Forestry and Technology, Changsha 410004, ChinaHunan Key Laboratory of Processed Food for Special Medical Purpose, Hunan Key Laboratory of Grain-Oil Deep Process and Quality Control, College of Food Science and Engineering, National Engineering Laboratory for Deep Process of Rice and Byproducts, Central South University of Forestry and Technology, Changsha 410004, ChinaHunan Key Laboratory of Processed Food for Special Medical Purpose, Hunan Key Laboratory of Grain-Oil Deep Process and Quality Control, College of Food Science and Engineering, National Engineering Laboratory for Deep Process of Rice and Byproducts, Central South University of Forestry and Technology, Changsha 410004, ChinaKey Laboratory of Biological Nanomaterials and Devices, College of life sciences and chemistry, Hunan University of Technology, Zhuzhou 412007, ChinaNano Ag has excellent antibacterial properties and is widely used in various antibacterial materials, such as antibacterial medicine and medical devices, food packaging materials and antibacterial textiles. Despite the many benefits of nano-Ag, more and more research indicates that it may have potential biotoxic effects. Studies have shown that people who ingest nanoparticles by mouth have the highest uptake in the intestinal tract, and that the colon area is the most vulnerable to damage and causes the disease. In this study, we examined the toxic effects of different concentrations of Ag-NPs on normal human colon cells (NCM460) and human colon cancer cells (HCT116). As the concentration of nanoparticles increased, the activity of the two colon cells decreased and intracellular reactive oxygen species (ROS) increased. RT-qPCR and Western-blot analyses showed that Ag NPs can promote the increase in P38 protein phosphorylation levels in two colon cells and promote the expression of P53 and Bax. The analysis also showed that Ag NPs can promote the down-regulation of Bcl-2, leading to an increased Bax/Bcl-2 ratio and activation of P21, further accelerating cell death. This study showed that a low concentration of nano Ag has no obvious toxic effect on colon cells, while nano Ag with concentrations higher than 15 μg/mL will cause oxidative damage to colon cells.https://www.mdpi.com/1422-0067/21/5/1618nano-agcolon cellsbiological toxicityoxidative damage |