A New Method for DNA Isolation from Bone Marrow & Sperms of White Mice
Background: A quick and easy method was developed for extraction of DNA of eukaryotes from different samples, which are bone marrow and sperms in white mice Mus musculus strain (Balb/c). Patients and Methods: this method using high salt buffer, Ethylene diemine tetracetec acid (EDTA), Trypsine,Sodi...
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| Format: | Article |
| Language: | English |
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Faculty of Medicine University of Baghdad
2009-07-01
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| Series: | مجلة كلية الطب |
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| Online Access: | http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/1217 |
| Summary: | Background: A quick and easy method was developed for extraction of DNA of eukaryotes from different samples, which are bone marrow and sperms in white mice Mus musculus strain (Balb/c).
Patients and Methods: this method using high salt buffer, Ethylene diemine tetracetec acid (EDTA), Trypsine,Sodium Dodecyl Sulfate(SDS), and urea without using Proteinase-K digestion or ultracentrifugation.
Results: This method was successful in extracting DNA from different samples in eukaryotic and this DNA is suitable for Hind III digestion.
Conclusion: Without further clean-up, the extracted DNA can be used for restriction endonuclease digestion or for numerous applications.
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| ISSN: | 0041-9419 2410-8057 |