A New Method for DNA Isolation from Bone Marrow & Sperms of White Mice

A New Method for DNA Isolation from Bone Marrow & Sperms of White Mice

Background: A quick and easy method was developed for extraction of DNA of eukaryotes from different samples, which are bone marrow and sperms in white mice Mus musculus strain (Balb/c). Patients and Methods: this method using high salt buffer, Ethylene diemine tetracetec acid (EDTA), Trypsine,Sodi...

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Main Author: Shayma J. Ahmed
Format: Article
Language:English
Published: Faculty of Medicine University of Baghdad 2009-07-01
Series:مجلة كلية الطب
Subjects:
DNA, Eukaryotes, extraction, Mice, bone marrow, Sperm, Hind III digestion.
Online Access:http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/1217
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spelling doaj-7705f14f51984d138a99319b4d3a9f6f2020-11-25T01:59:19ZengFaculty of Medicine University of Baghdadمجلة كلية الطب0041-94192410-80572009-07-0151210.32007/1217%g220-222A New Method for DNA Isolation from Bone Marrow & Sperms of White MiceShayma J. Ahmed0Department of Anatomy, college of Medicine, university of Baghdad Background: A quick and easy method was developed for extraction of DNA of eukaryotes from different samples, which are bone marrow and sperms in white mice Mus musculus strain (Balb/c). Patients and Methods: this method using high salt buffer, Ethylene diemine tetracetec acid (EDTA), Trypsine,Sodium Dodecyl Sulfate(SDS), and urea without using Proteinase-K digestion or ultracentrifugation. Results: This method was successful in extracting DNA from different samples in eukaryotic and this DNA is suitable for Hind III digestion. Conclusion: Without further clean-up, the extracted DNA can be used for restriction endonuclease digestion or for numerous applications. http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/1217DNA, Eukaryotes, extraction, Mice, bone marrow, Sperm, Hind III digestion.
collection DOAJ
language English
format Article
sources DOAJ
author Shayma J. Ahmed
spellingShingle Shayma J. Ahmed
A New Method for DNA Isolation from Bone Marrow & Sperms of White Mice
مجلة كلية الطب
DNA, Eukaryotes, extraction, Mice, bone marrow, Sperm, Hind III digestion.
author_facet Shayma J. Ahmed
author_sort Shayma J. Ahmed
title A New Method for DNA Isolation from Bone Marrow & Sperms of White Mice
title_short A New Method for DNA Isolation from Bone Marrow & Sperms of White Mice
title_full A New Method for DNA Isolation from Bone Marrow & Sperms of White Mice
title_fullStr A New Method for DNA Isolation from Bone Marrow & Sperms of White Mice
title_full_unstemmed A New Method for DNA Isolation from Bone Marrow & Sperms of White Mice
title_sort new method for dna isolation from bone marrow & sperms of white mice
publisher Faculty of Medicine University of Baghdad
series مجلة كلية الطب
issn 0041-9419
2410-8057
publishDate 2009-07-01
description Background: A quick and easy method was developed for extraction of DNA of eukaryotes from different samples, which are bone marrow and sperms in white mice Mus musculus strain (Balb/c). Patients and Methods: this method using high salt buffer, Ethylene diemine tetracetec acid (EDTA), Trypsine,Sodium Dodecyl Sulfate(SDS), and urea without using Proteinase-K digestion or ultracentrifugation. Results: This method was successful in extracting DNA from different samples in eukaryotic and this DNA is suitable for Hind III digestion. Conclusion: Without further clean-up, the extracted DNA can be used for restriction endonuclease digestion or for numerous applications.
topic DNA, Eukaryotes, extraction, Mice, bone marrow, Sperm, Hind III digestion.
url http://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/1217
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