Evaluation of Zymodemes of Pathogen and Nonpathogen Entamoeba histolytica

• Main Authors: Nurettin ARDIÇ, Mehmet TANYÜKSEL, Levent DOĞANCI, Hüseyin GÜN
• Format: Article
• Language: English
• Published: Bilimsel Tip Yayinevi 1998-06-01
• Series: Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi
• Subjects: Entamoeba histolytica; E. dispar; Zymodeme; Isoenzyme
• Online Access: http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=1998-3-2-125-133.pdf

Entamoeba histolytica is the protozoan responsible for human amoebosis. It infects 10% of the world population, causing 50 million cases of dysentery or liver abscesses and to death of 100.000 humans per year. As with other parasitic infections, amoebosis is a consequence of poverty and bad sanitary conditions. According to epidemiological studies, only 10% of the effected people are infected with pathogenic strains. By enzyme electrophoresis, 23 zymodemes of E. histolytica have been identified. Of these, nine have been associated with pathology in the host. In this study, seven E. histolytica isolates were analized for their zymodeme patterns and the enzymes used to classify E. histolytica include glucosphosphate isomerase (GPI), hexokinase (HK), L-Malate (ME), and phosphoglucomutase (PGM) by observing the migration pattern of isoenzyme bands in polyacrylamide gel electrophoresis (PAGE). Our isolates were taken from different geographical parts; Diyarbakır (four isolates), Izmir (two isolates) and Ankara (one isolate) regions. HK enzyme is characterized by two fast-migrating bands in four isolates of symptomatic patients (three isolates from Diyarbakır region, and one isolate from Izmir region) and one isolate from on asymptomatic patient. PGM band were observed in three isolates (Diyarbakır region). GPI band was observedas in two isolates (Diyarbak›r region), whereas as a beta band in one isolate (Diyarbakır region). ME band is not found in any of the isolates.