Biased genome editing using the local accumulation of DSB repair molecules system
Genome editing using CRISPR can be enhanced by manipulating DNA double-strand break repair pathways. Here the authors demonstrate LoAD, local accumulation of repair molecules, which shifts repair to microhomology-mediated end-joining.
Main Authors: | Shota Nakade, Keiji Mochida, Atsushi Kunii, Kazuki Nakamae, Tomomi Aida, Kohichi Tanaka, Naoaki Sakamoto, Tetsushi Sakuma, Takashi Yamamoto |
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Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2018-08-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-018-05773-6 |
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